Gene description for DAPK1
Gene name death-associated protein kinase 1
Gene symbol DAPK1
Other names/aliases DAPK
Species Homo sapiens
 Database cross references - DAPK1
ExoCarta ExoCarta_1612
Vesiclepedia VP_1612
Entrez Gene 1612
HGNC 2674
MIM 600831
UniProt P53355  
 DAPK1 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for DAPK1
Molecular Function
    calmodulin binding GO:0005516 IDA
    syntaxin-1 binding GO:0017075 IPI
    GTP binding GO:0005525 IEA
    identical protein binding GO:0042802 IPI
    ATP binding GO:0005524 IDA
    calmodulin-dependent protein kinase activity GO:0004683 IDA
    protein binding GO:0005515 IPI
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 TAS
Biological Process
    intracellular signal transduction GO:0035556 IDA
    negative regulation of translation GO:0017148 IDA
    negative regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902042 IEA
    protein autophosphorylation GO:0046777 TAS
    cellular response to interferon-gamma GO:0071346 IDA
    apoptotic signaling pathway GO:0097190 IMP
    extrinsic apoptotic signaling pathway in absence of ligand GO:0097192 TAS
    regulation of N-methyl-D-aspartate selective glutamate receptor activity GO:2000310 ISS
    positive regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043280 IDA
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 IEA
    regulation of apoptotic process GO:0042981 TAS
    extrinsic apoptotic signaling pathway GO:0097191 TAS
    regulation of autophagy GO:0010506 TAS
    protein phosphorylation GO:0006468 TAS
    apoptotic process GO:0006915 TAS
    programmed cell death GO:0012501 TAS
Subcellular Localization
    actin cytoskeleton GO:0015629 IDA
    plasma membrane GO:0005886 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified DAPK1 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DAPK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAQ 10971
Affinity Capture-Western Homo sapiens
2 KRIT1 889
Affinity Capture-Western Homo sapiens
3 FADD 8772
Affinity Capture-Western Homo sapiens
4 TNFRSF1A 7132
Affinity Capture-Western Homo sapiens
5 MIB1 57534
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which DAPK1 is involved
PathwayEvidenceSource
Ligand-independent caspase activation via DCC TAS Reactome





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