Gene description for PLCB1
Gene name phospholipase C, beta 1 (phosphoinositide-specific)
Gene symbol PLCB1
Other names/aliases EIEE12
PI-PLC
PLC-154
PLC-I
PLC154
PLCB1A
PLCB1B
Species Homo sapiens
 Database cross references - PLCB1
ExoCarta ExoCarta_23236
Vesiclepedia VP_23236
Entrez Gene 23236
HGNC 15917
MIM 607120
UniProt Q9NQ66  
 PLCB1 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Saliva 19199708    
 Gene ontology annotations for PLCB1
Molecular Function
    phosphatidylinositol phospholipase C activity GO:0004435 IBA
    phosphatidylinositol phospholipase C activity GO:0004435 IDA
    phosphatidylinositol phospholipase C activity GO:0004435 ISS
    phosphatidylinositol phospholipase C activity GO:0004435 NAS
    phospholipase C activity GO:0004629 TAS
    GTPase activator activity GO:0005096 IDA
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IBA
    calmodulin binding GO:0005516 IDA
    lamin binding GO:0005521 IEA
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IDA
    enzyme binding GO:0019899 IPI
    identical protein binding GO:0042802 ISS
Biological Process
    G2/M transition of mitotic cell cycle GO:0000086 ISS
    signal transduction GO:0007165 NAS
    G protein-coupled receptor signaling pathway GO:0007186 IBA
    G protein-coupled receptor signaling pathway GO:0007186 TAS
    phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway GO:0007207 IEA
    G protein-coupled acetylcholine receptor signaling pathway GO:0007213 ISS
    glutamate receptor signaling pathway GO:0007215 ISS
    learning GO:0007612 IEA
    memory GO:0007613 IBA
    memory GO:0007613 ISS
    regulation of G protein-coupled receptor signaling pathway GO:0008277 ISS
    cerebral cortex development GO:0021987 ISS
    phosphatidylinositol catabolic process GO:0031161 IEA
    positive regulation of insulin secretion GO:0032024 IEA
    positive regulation of interleukin-12 production GO:0032735 ISS
    inositol trisphosphate metabolic process GO:0032957 IEA
    interleukin-12-mediated signaling pathway GO:0035722 IDA
    interleukin-15-mediated signaling pathway GO:0035723 IDA
    positive regulation of embryonic development GO:0040019 ISS
    fat cell differentiation GO:0045444 IEA
    positive regulation of myoblast differentiation GO:0045663 ISS
    negative regulation of DNA-templated transcription GO:0045892 ISS
    positive regulation of DNA-templated transcription GO:0045893 ISS
    positive regulation of JNK cascade GO:0046330 IDA
    phosphatidylinositol metabolic process GO:0046488 IBA
    phosphatidylinositol metabolic process GO:0046488 IDA
    phosphatidylinositol metabolic process GO:0046488 ISS
    insulin-like growth factor receptor signaling pathway GO:0048009 ISS
    phosphatidylinositol-mediated signaling GO:0048015 IBA
    positive regulation of developmental growth GO:0048639 ISS
    release of sequestered calcium ion into cytosol GO:0051209 IBA
    activation of meiosis involved in egg activation GO:0060466 ISS
    interleukin-1-mediated signaling pathway GO:0070498 IDA
    regulation of fertilization GO:0080154 ISS
    ion channel modulating, G protein-coupled receptor signaling pathway GO:0099105 IEA
    postsynaptic modulation of chemical synaptic transmission GO:0099170 IEA
    regulation of retrograde trans-synaptic signaling by endocanabinoid GO:0099178 IEA
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 ISS
    cellular response to fluoride GO:1902618 IEA
    regulation of establishment of endothelial barrier GO:1903140 IEA
    cellular response to vasopressin GO:1904117 IEA
    cellular response to ionomycin GO:1904637 IEA
    cellular response to glyceraldehyde GO:1905631 IEA
    ligand-gated ion channel signaling pathway GO:1990806 IEA
    positive regulation of acrosome reaction GO:2000344 ISS
    negative regulation of monocyte extravasation GO:2000438 ISS
    positive regulation of CD24 production GO:2000560 ISS
Subcellular Localization
    chromatin GO:0000785 ISS
    nucleus GO:0005634 NAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    nuclear speck GO:0016607 IBA
    nuclear speck GO:0016607 IDA
    nuclear membrane GO:0031965 IEA
    protein-containing complex GO:0032991 IEA
    extracellular exosome GO:0070062 HDA
    glutamatergic synapse GO:0098978 IEA
    GABA-ergic synapse GO:0098982 IEA
    postsynaptic cytosol GO:0099524 IEA
 Experiment description of studies that identified PLCB1 in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for PLCB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SPTBN1 6711
Two-hybrid Homo sapiens
2 GAN 8139
Affinity Capture-MS Homo sapiens
3 TFCP2 7024
Two-hybrid Homo sapiens
4 PCF11 51585
Co-fractionation Homo sapiens
5 PRNP 5621
Affinity Capture-MS Homo sapiens
6 GNAQ 2776
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
7 PLCB1 23236
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
8 CTBP2 1488
Two-hybrid Homo sapiens
9 LAMP1 3916
Proximity Label-MS Homo sapiens
10 RAB35 11021
Proximity Label-MS Homo sapiens
11 NR3C1 2908
Proximity Label-MS Homo sapiens
12 LAMP2 3920
Proximity Label-MS Homo sapiens
13 ARF6 382
Proximity Label-MS Homo sapiens
14 CTBP1 1487
Two-hybrid Homo sapiens
15 C11orf52 91894
Proximity Label-MS Homo sapiens
16 PTGER3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 LYN 4067
Proximity Label-MS Homo sapiens
18 MCAM 4162
Proximity Label-MS Homo sapiens
19 APEX1 328
Affinity Capture-RNA Homo sapiens
20 KCNE3  
Affinity Capture-MS Homo sapiens
21 GNA11 2767
Affinity Capture-Western Homo sapiens
22 RAB2A 5862
Proximity Label-MS Homo sapiens
23 BTN2A1 11120
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 KRIT1 889
Affinity Capture-Western Homo sapiens
25 TRPM7 54822
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
26 SLC9A3R1 9368
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 EPHA2 1969
Proximity Label-MS Homo sapiens
28 KRAS 3845
Proximity Label-MS Homo sapiens
29 RHOB 388
Proximity Label-MS Homo sapiens
30 CEP76  
Two-hybrid Homo sapiens
31 DEF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PLCB1 is involved
PathwayEvidenceSource
Acetylcholine regulates insulin secretion TAS Reactome
Acetylcholine regulates insulin secretion IEA Reactome
Activation of kainate receptors upon glutamate binding TAS Reactome
Beta-catenin independent WNT signaling TAS Reactome
Ca2+ pathway TAS Reactome
Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion TAS Reactome
Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion IEA Reactome
Free fatty acids regulate insulin secretion TAS Reactome
Free fatty acids regulate insulin secretion IEA Reactome
G alpha (i) signalling events TAS Reactome
G alpha (i) signalling events IEA Reactome
G alpha (q) signalling events TAS Reactome
G alpha (q) signalling events IEA Reactome
G beta:gamma signalling through PLC beta TAS Reactome
G-protein beta:gamma signalling TAS Reactome
G-protein mediated events TAS Reactome
G-protein mediated events IEA Reactome
GPCR downstream signalling TAS Reactome
GPCR downstream signalling IEA Reactome
Inositol phosphate metabolism TAS Reactome
Integration of energy metabolism TAS Reactome
Integration of energy metabolism IEA Reactome
Metabolism TAS Reactome
Metabolism IEA Reactome
Neuronal System TAS Reactome
Neurotransmitter receptors and postsynaptic signal transmission TAS Reactome
Opioid Signalling TAS Reactome
Opioid Signalling IEA Reactome
PLC beta mediated events TAS Reactome
PLC beta mediated events IEA Reactome
Presynaptic function of Kainate receptors TAS Reactome
Regulation of insulin secretion TAS Reactome
Regulation of insulin secretion IEA Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by GPCR TAS Reactome
Signaling by GPCR IEA Reactome
Signaling by WNT TAS Reactome
Synthesis of IP3 and IP4 in the cytosol TAS Reactome
Transmission across Chemical Synapses TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here