Gene description for PLCB1
Gene name phospholipase C, beta 1 (phosphoinositide-specific)
Gene symbol PLCB1
Other names/aliases EIEE12
PI-PLC
PLC-154
PLC-I
PLC154
PLCB1A
PLCB1B
Species Homo sapiens
 Database cross references - PLCB1
ExoCarta ExoCarta_23236
Vesiclepedia VP_23236
Entrez Gene 23236
HGNC 15917
MIM 607120
UniProt Q9NQ66  
 PLCB1 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Saliva 19199708    
 Gene ontology annotations for PLCB1
Molecular Function
    protein binding GO:0005515 IPI
    signal transducer activity GO:0004871 IEA
    protein homodimerization activity GO:0042803 ISS
    enzyme binding GO:0019899 IPI
    GTPase activator activity GO:0005096 IDA
    phosphatidylinositol phospholipase C activity GO:0004435 NAS
    lamin binding GO:0005521 IEA
    calmodulin binding GO:0005516 IPI
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IDA
    calcium ion binding GO:0005509 IEA
Biological Process
    interleukin-15-mediated signaling pathway GO:0035723 IDA
    positive regulation of myoblast differentiation GO:0045663 ISS
    interleukin-1-mediated signaling pathway GO:0070498 IDA
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 ISS
    insulin-like growth factor receptor signaling pathway GO:0048009 ISS
    regulation of fertilization GO:0080154 ISS
    inositol phosphate metabolic process GO:0043647 TAS
    glutamate receptor signaling pathway GO:0007215 ISS
    positive regulation of CD24 biosynthetic process GO:2000560 ISS
    positive regulation of GTPase activity GO:0043547 IDA
    signal transduction GO:0007165 NAS
    positive regulation of interleukin-12 production GO:0032735 ISS
    positive regulation of transcription, DNA-templated GO:0045893 ISS
    memory GO:0007613 ISS
    fat cell differentiation GO:0045444 IEA
    G-protein coupled acetylcholine receptor signaling pathway GO:0007213 ISS
    negative regulation of monocyte extravasation GO:2000438 ISS
    positive regulation of acrosome reaction GO:2000344 ISS
    positive regulation of JNK cascade GO:0046330 IDA
    positive regulation of developmental growth GO:0048639 ISS
    intracellular signal transduction GO:0035556 IEA
    phosphatidylinositol metabolic process GO:0046488 ISS
    cerebral cortex development GO:0021987 ISS
    interleukin-12-mediated signaling pathway GO:0035722 IDA
    lipid catabolic process GO:0016042 IEA
    activation of meiosis involved in egg activation GO:0060466 ISS
    small molecule metabolic process GO:0044281 TAS
    metabolic process GO:0008152 ISS
    G2/M transition of mitotic cell cycle GO:0000086 ISS
    negative regulation of transcription, DNA-templated GO:0045892 ISS
    positive regulation of embryonic development GO:0040019 ISS
    synaptic transmission GO:0007268 TAS
    regulation of G-protein coupled receptor protein signaling pathway GO:0008277 ISS
Subcellular Localization
    nuclear chromatin GO:0000790 ISS
    cytosol GO:0005829 TAS
    nucleus GO:0005634 NAS
    nuclear membrane GO:0031965 IEA
    extracellular exosome GO:0070062 IDA
    nuclear speck GO:0016607 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified PLCB1 in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 66
MISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|Alix|CD63|CD81
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for PLCB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNAQ 2776
Affinity Capture-Western Homo sapiens
2 TRPM7 54822
Invitro Homo sapiens
Two-hybrid Homo sapiens
3 SLC9A3R1 9368
Affinity Capture-Western Homo sapiens
4 RGS4  
Invitro Homo sapiens
View the network image/svg+xml



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