Gene description for BNIP3
Gene name BCL2/adenovirus E1B 19kDa interacting protein 3
Gene symbol BNIP3
Other names/aliases NIP3
Species Homo sapiens
 Database cross references - BNIP3
ExoCarta ExoCarta_664
Vesiclepedia VP_664
Entrez Gene 664
HGNC 1084
MIM 603293
UniProt Q12983  
 BNIP3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for BNIP3
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    GTPase binding GO:0051020 IPI
    endoplasmic reticulum-autophagosome adaptor activity GO:0140506 IDA
    mitochondrion autophagosome adaptor activity GO:0140580 IDA
Biological Process
    autophagy of mitochondrion GO:0000422 NAS
    mitophagy GO:0000423 IMP
    response to hypoxia GO:0001666 ISS
    response to bacterium GO:0009617 IEA
    positive regulation of autophagy GO:0010508 TAS
    negative regulation of mitochondrial fusion GO:0010637 IDA
    cardiac muscle cell apoptotic process GO:0010659 IEA
    positive regulation of cardiac muscle cell apoptotic process GO:0010666 IEA
    negative regulation of mitochondrial membrane potential GO:0010917 IEA
    positive regulation of macroautophagy GO:0016239 IGI
    cerebral cortex development GO:0021987 IEA
    mitochondrial protein catabolic process GO:0035694 IMP
    positive regulation of apoptotic process GO:0043065 IDA
    negative regulation of apoptotic process GO:0043066 TAS
    positive regulation of programmed cell death GO:0043068 IBA
    positive regulation of programmed cell death GO:0043068 IDA
    negative regulation of programmed cell death GO:0043069 IGI
    positive regulation of protein-containing complex disassembly GO:0043243 IDA
    mitochondrial fragmentation involved in apoptotic process GO:0043653 IDA
    negative regulation of membrane potential GO:0045837 IDA
    regulation of mitochondrial membrane permeability GO:0046902 IDA
    autophagic cell death GO:0048102 IEA
    response to axon injury GO:0048678 IEA
    oligodendrocyte differentiation GO:0048709 IEA
    brown fat cell differentiation GO:0050873 IEA
    neuron apoptotic process GO:0051402 ISS
    positive regulation of mitochondrial calcium ion concentration GO:0051561 IEA
    defense response to virus GO:0051607 IDA
    response to hyperoxia GO:0055093 IEA
    reticulophagy GO:0061709 IMP
    cellular response to hydrogen peroxide GO:0070301 IEA
    cellular response to mechanical stimulus GO:0071260 IEP
    cellular response to cobalt ion GO:0071279 IMP
    cellular response to hypoxia GO:0071456 IGI
    cellular response to hypoxia GO:0071456 IMP
    reactive oxygen species metabolic process GO:0072593 IDA
    positive regulation of mitochondrial fission GO:0090141 IDA
    positive regulation of release of cytochrome c from mitochondria GO:0090200 IDA
    response to oxygen-glucose deprivation GO:0090649 IEA
    mitochondrial outer membrane permeabilization GO:0097345 IBA
    mitochondrial outer membrane permeabilization GO:0097345 IDA
    granzyme-mediated programmed cell death signaling pathway GO:0140507 IDA
    negative regulation of mitochondrial membrane permeability involved in apoptotic process GO:1902109 IEA
    positive regulation of autophagy of mitochondrion GO:1903599 IEA
    regulation of aerobic respiration GO:1903715 IEA
    intrinsic apoptotic signaling pathway in response to hypoxia GO:1990144 IMP
    negative regulation of reactive oxygen species metabolic process GO:2000378 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISS
    nuclear envelope GO:0005635 IBA
    nuclear envelope GO:0005635 IDA
    nucleoplasm GO:0005654 ISS
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 TAS
    mitochondrial outer membrane GO:0005741 IBA
    mitochondrial outer membrane GO:0005741 IDA
    mitochondrial outer membrane GO:0005741 IDA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 NAS
    endoplasmic reticulum membrane GO:0005789 IDA
    postsynaptic density GO:0014069 IEA
    dendrite GO:0030425 ISS
    mitochondrial membrane GO:0031966 IDA
    mitochondrial membrane GO:0031966 IMP
 Experiment description of studies that identified BNIP3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for BNIP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Affinity Capture-MS Homo sapiens
2 SLC31A2  
Two-hybrid Homo sapiens
3 NCBP1 4686
Two-hybrid Homo sapiens
4 MTCH1 23787
Proximity Label-MS Homo sapiens
5 HPN  
Two-hybrid Homo sapiens
6 TMEM205 374882
Two-hybrid Homo sapiens
7 BNIP3L  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
8 MS4A3  
Two-hybrid Homo sapiens
9 AMIGO1  
Two-hybrid Homo sapiens
10 MAP1LC3B 81631
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
11 PPTC7 160760
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
12 RHEB 6009
Two-hybrid Homo sapiens
FRET Homo sapiens
13 TMPRSS2 7113
Two-hybrid Homo sapiens
14 RPRM  
Two-hybrid Homo sapiens
15 CD3E 916
Two-hybrid Homo sapiens
16 C10orf35  
Two-hybrid Homo sapiens
17 AGTR1  
Two-hybrid Homo sapiens
18 HIATL1 84641
Two-hybrid Homo sapiens
19 CLDN9  
Two-hybrid Homo sapiens
20 SEC23A 10484
Two-hybrid Homo sapiens
21 CLN8 2055
Two-hybrid Homo sapiens
22 EBAG9 9166
Two-hybrid Homo sapiens
23 SLC35B1 10237
Two-hybrid Homo sapiens
24 TMEM106C 79022
Two-hybrid Homo sapiens
25 MTCH2 23788
Proximity Label-MS Homo sapiens
26 CLEC7A  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
27 TGM2 7052
Two-hybrid Homo sapiens
28 SLC6A17 388662
Two-hybrid Homo sapiens
29 JAGN1 84522
Two-hybrid Homo sapiens
30 HIVEP1 3096
Two-hybrid Homo sapiens
31 USMG5 84833
Affinity Capture-MS Homo sapiens
32 BCL2L2 599
Two-hybrid Homo sapiens
33 OPA1 4976
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
34 CREB3  
Two-hybrid Homo sapiens
35 BCL2L1 598
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
36 TMEM56  
Two-hybrid Homo sapiens
37 ARL13B 200894
Two-hybrid Homo sapiens
38 SCN3B  
Two-hybrid Homo sapiens
39 BAG3 9531
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
40 FBXL4 26235
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
41 RNF24  
Two-hybrid Homo sapiens
42 TMEM30A 55754
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 RNASEK  
Two-hybrid Homo sapiens
44 HTRA2 27429
Affinity Capture-MS Homo sapiens
45 FFAR2  
Two-hybrid Homo sapiens
46 SMEK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 TM4SF18  
Two-hybrid Homo sapiens
48 MTX2 10651
Proximity Label-MS Homo sapiens
49 ERGIC3 51614
Two-hybrid Homo sapiens
50 NR3C1 2908
Proximity Label-MS Homo sapiens
51 MYO19  
Affinity Capture-MS Homo sapiens
52 FXYD6-FXYD2  
Two-hybrid Homo sapiens
53 TMEM11 8834
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
54 CREB3L1  
Two-hybrid Homo sapiens
55 SPACA1 81833
Two-hybrid Homo sapiens
56 GPR152  
Two-hybrid Homo sapiens
57 FAM209A 200232
Two-hybrid Homo sapiens
58 GPR42  
Two-hybrid Homo sapiens
59 CMTM5  
Two-hybrid Homo sapiens
60 HTR2B  
Two-hybrid Homo sapiens
61 PLP2 5355
Two-hybrid Homo sapiens
62 KTN1 3895
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
63 BNIP3 664
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
64 GPR37  
Two-hybrid Homo sapiens
65 FATE1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
66 RAC2 5880
Affinity Capture-MS Homo sapiens
67 AKAP1 8165
Proximity Label-MS Homo sapiens
68 ZDHHC15 158866
Two-hybrid Homo sapiens
69 MALL  
Two-hybrid Homo sapiens
70 TMX2 51075
Two-hybrid Homo sapiens
71 IFNGR2  
Two-hybrid Homo sapiens
72 SMIM3  
Two-hybrid Homo sapiens
73 KIF14 9928
Affinity Capture-MS Homo sapiens
74 LDLRAD1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
75 CD47 961
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
76 SQSTM1 8878
Affinity Capture-Western Homo sapiens
77 HDAC1 3065
Affinity Capture-Western Homo sapiens
78 EBP  
Two-hybrid Homo sapiens
79 REEP2  
Two-hybrid Homo sapiens
80 MAP1LC3A 84557
Affinity Capture-Western Homo sapiens
81 ABTB2 25841
Affinity Capture-MS Homo sapiens
82 BCL2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
83 SEC22A  
Two-hybrid Homo sapiens
84 BNIP2  
Two-hybrid Homo sapiens
85 ENPEP 2028
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 ELOVL4  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which BNIP3 is involved
No pathways found





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