Gene description for BNIP3
Gene name BCL2/adenovirus E1B 19kDa interacting protein 3
Gene symbol BNIP3
Other names/aliases NIP3
Species Homo sapiens
 Database cross references - BNIP3
ExoCarta ExoCarta_664
Vesiclepedia VP_664
Entrez Gene 664
HGNC 1084
MIM 603293
UniProt Q12983  
 BNIP3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for BNIP3
Molecular Function
    GTPase binding GO:0051020 IPI
    protein homodimerization activity GO:0042803 NAS
    protein heterodimerization activity GO:0046982 IDA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    response to hypoxia GO:0001666 ISS
    cell death GO:0008219 ISS
    cellular response to cobalt ion GO:0071279 IMP
    cellular response to hydrogen peroxide GO:0070301 IEA
    autophagic cell death GO:0048102 IEA
    positive regulation of protein complex disassembly GO:0043243 IDA
    mitochondrial outer membrane permeabilization GO:0097345 IDA
    toxin transport GO:1901998 IEA
    positive regulation of apoptotic process GO:0043065 IDA
    positive regulation of mitochondrial fission GO:0090141 IDA
    response to hyperoxia GO:0055093 IEA
    positive regulation of programmed cell death GO:0043068 IDA
    mitochondrion degradation GO:0000422 NAS
    negative regulation of apoptotic process GO:0043066 TAS
    brown fat cell differentiation GO:0050873 IEA
    neuron apoptotic process GO:0051402 ISS
    mitochondrial fragmentation involved in apoptotic process GO:0043653 IDA
    apoptotic process GO:0006915 IPI
    positive regulation of release of cytochrome c from mitochondria GO:0090200 IDA
    negative regulation of membrane potential GO:0045837 IDA
    granzyme-mediated apoptotic signaling pathway GO:0008626 IDA
    viral process GO:0016032 IEA
    cellular response to mechanical stimulus GO:0071260 IEP
    mitochondrial protein catabolic process GO:0035694 IMP
    defense response to virus GO:0051607 IDA
    positive regulation of autophagy GO:0010508 TAS
    cellular response to hypoxia GO:0071456 IMP
    intrinsic apoptotic signaling pathway in response to hypoxia GO:1990144 IMP
    negative regulation of mitochondrial fusion GO:0010637 IDA
    regulation of mitochondrial membrane permeability GO:0046902 IDA
    reactive oxygen species metabolic process GO:0072593 IDA
Subcellular Localization
    nuclear envelope GO:0005635 IDA
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 ISS
    endoplasmic reticulum GO:0005783 NAS
    dendrite GO:0030425 ISS
    integral component of mitochondrial outer membrane GO:0031307 IDA
    mitochondrion GO:0005739 TAS
    cytoplasm GO:0005737 ISS
    mitochondrial outer membrane GO:0005741 IDA
    mitochondrial membrane GO:0031966 IMP
 Experiment description of studies that identified BNIP3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for BNIP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BCL2  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
2 BNIP3 664
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
3 CD47 961
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
4 NCBP1 4686
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
5 BCL2L1 598
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
6 BNIP3L  
Two-hybrid Homo sapiens
7 TMEM11 8834
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which BNIP3 is involved
No pathways found





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