Experiment description of studies that identified CLN8 in exosomes

1 Experiment ID 226 MISEV standards ✔ EM Biophysical techniques ✔ GAPDH|CD9|FLOT1 Enriched markers ✔ LMNA|H2AFX|ATP5A1|TOMM20 Negative markers ✘ Particle analysis Identified molecule mRNA Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa) PubMed ID 26027894     Organism Homo sapiens Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting" Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar" Journal name Journal of Extracellular Vesicles Publication year 2015 Sample Endothelial cells Sample name HMEC-1 Isolation/purification methods Differential ultracentrifugation Sucrose density gradient Flotation density 1.10 g/mL Molecules identified in the study miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting

2 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

Protein-protein interactions for CLN8

Protein Interactor ExoCarta ID Identification method PubMed Species 1 TMEM128   Two-hybrid Homo sapiens 2 TMEM107 84314 Two-hybrid Homo sapiens 3 FZD7 8324 Two-hybrid Homo sapiens 4 VAPA 9218 Affinity Capture-Western Homo sapiens Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens 5 PLP1 5354 Two-hybrid Homo sapiens 6 C14orf1 11161 Two-hybrid Homo sapiens 7 YIPF6 286451 Two-hybrid Homo sapiens 8 MTX1 4580 Two-hybrid Homo sapiens 9 ARL6IP5 10550 Two-hybrid Homo sapiens 10 TMEM134   Two-hybrid Homo sapiens 11 TMEM147 10430 Two-hybrid Homo sapiens 12 GABARAPL2 11345 Two-hybrid Homo sapiens 13 CD9 928 Two-hybrid Homo sapiens 14 UBC 7316 Two-hybrid Homo sapiens 15 BNIP3L   Two-hybrid Homo sapiens 16 TMEM11 8834 Two-hybrid Homo sapiens 17 RTN4 57142 Two-hybrid Homo sapiens 18 TUBB4A 10382 Two-hybrid Homo sapiens 19 RRP15   Two-hybrid Homo sapiens 20 NDRG2 57447 Two-hybrid Homo sapiens 21 CYB5B 80777 Two-hybrid Homo sapiens 22 PTPRN   Two-hybrid Homo sapiens 23 ZUFSP 221302 Two-hybrid Homo sapiens 24 STX8 9482 Two-hybrid Homo sapiens 25 PLP2 5355 Two-hybrid Homo sapiens 26 SELK   Two-hybrid Homo sapiens 27 BNIP3 664 Two-hybrid Homo sapiens 28 TMEM14A   Two-hybrid Homo sapiens 29 SPCS2 9789 Two-hybrid Homo sapiens 30 GPM6B 2824 Two-hybrid Homo sapiens 31 PDIA6 10130 Two-hybrid Homo sapiens View the network

Pathways in which CLN8 is involved

No pathways found