Gene description for STX8
Gene name syntaxin 8
Gene symbol STX8
Other names/aliases CARB
Species Homo sapiens
 Database cross references - STX8
ExoCarta ExoCarta_9482
Entrez Gene 9482
HGNC 11443
MIM 604203
UniProt Q9UNK0  
 STX8 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for STX8
Molecular Function
    protein binding GO:0005515 IPI
    SNAP receptor activity GO:0005484 IBA
    ubiquitin protein ligase binding GO:0031625 IEA
    chloride channel inhibitor activity GO:0019869 IDA
    syntaxin binding GO:0019905 IPI
Biological Process
    transport GO:0006810 TAS
    regulation of protein localization to plasma membrane GO:1903076 IDA
    intracellular protein transport GO:0006886 IBA
    early endosome to late endosome transport GO:0045022 IDA
    endosome to lysosome transport GO:0008333 IEA
    vesicle fusion GO:0006906 IBA
Subcellular Localization
    vesicle GO:0031982 IDA
    SNARE complex GO:0031201 IBA
    trans-Golgi network GO:0005802 IDA
    integral component of plasma membrane GO:0005887 TAS
    endosome GO:0005768 IDA
    early endosome GO:0005769 IDA
    late endosome membrane GO:0031902 IEA
    lysosomal membrane GO:0005765 IEA
    late endosome GO:0005770 IDA
    recycling endosome GO:0055037 IDA
    endoplasmic reticulum GO:0005783 TAS
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified STX8 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for STX8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CREB3  
Two-hybrid Homo sapiens
2 STX7 8417
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 NAPG 8774
Affinity Capture-MS Homo sapiens
4 VTI1B 10490
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
5 VAMP8 8673
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
6 VTI1A 143187
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 COPA 1314
Invivo Homo sapiens
8 NAPA 8775
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which STX8 is involved
No pathways found





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