Gene description for VTI1A
Gene name vesicle transport through interaction with t-SNAREs 1A
Gene symbol VTI1A
Other names/aliases MMDS3
MVti1
VTI1RP2
Vti1-rp2
Species Homo sapiens
 Database cross references - VTI1A
ExoCarta ExoCarta_143187
Entrez Gene 143187
HGNC 17792
MIM 614316
UniProt Q96AJ9  
 VTI1A identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for VTI1A
Molecular Function
    SNAP receptor activity GO:0005484 IDA
Biological Process
    retrograde transport, endosome to Golgi GO:0042147 IMP
    Golgi ribbon formation GO:0090161 IMP
    ER to Golgi vesicle-mediated transport GO:0006888 ISS
    voluntary musculoskeletal movement GO:0050882 IMP
    intracellular protein transport GO:0006886 IEA
    vesicle fusion with Golgi apparatus GO:0048280 ISS
Subcellular Localization
    SNARE complex GO:0031201 TAS
    Golgi apparatus GO:0005794 IDA
    integral component of membrane GO:0016021 IEA
    Golgi membrane GO:0000139 IEA
    synaptic vesicle GO:0008021 ISS
    perinuclear region of cytoplasm GO:0048471 ISS
    neuron projection terminus GO:0044306 ISS
    clathrin-coated vesicle GO:0030136 ISS
    endosome GO:0005768 ISS
    neuronal cell body GO:0043025 ISS
 Experiment description of studies that identified VTI1A in exosomes
1
Experiment ID 126
ISEV standards
EV Biophysical techniques
GAPDH
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for VTI1A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VAMP8 8673
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
2 STX8 9482
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 VAMP4  
Affinity Capture-Western Homo sapiens
4 STX7 8417
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 VAMP3 9341
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which VTI1A is involved
No pathways found





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