Gene description for VTI1A
Gene name vesicle transport through interaction with t-SNAREs 1A
Gene symbol VTI1A
Other names/aliases MMDS3
MVti1
VTI1RP2
Vti1-rp2
Species Homo sapiens
 Database cross references - VTI1A
ExoCarta ExoCarta_143187
Vesiclepedia VP_143187
Entrez Gene 143187
HGNC 17792
MIM 614316
UniProt Q96AJ9  
 VTI1A identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells Unpublished / Not applicable
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for VTI1A
Molecular Function
    SNARE binding GO:0000149 IBA
    SNAP receptor activity GO:0005484 IBA
    SNAP receptor activity GO:0005484 IDA
    protein binding GO:0005515 IPI
Biological Process
    intracellular protein transport GO:0006886 IEA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 ISS
    intra-Golgi vesicle-mediated transport GO:0006891 IBA
    Golgi to vacuole transport GO:0006896 IBA
    autophagy GO:0006914 IMP
    synaptic vesicle to endosome fusion GO:0016189 IEA
    macroautophagy GO:0016236 IBA
    endocytic recycling GO:0032456 IMP
    retrograde transport, endosome to Golgi GO:0042147 IBA
    retrograde transport, endosome to Golgi GO:0042147 IDA
    retrograde transport, endosome to Golgi GO:0042147 IMP
    vesicle fusion with Golgi apparatus GO:0048280 IBA
    vesicle fusion with Golgi apparatus GO:0048280 ISS
    voluntary musculoskeletal movement GO:0050882 IMP
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    endosome GO:0005768 ISS
    autophagosome GO:0005776 IDA
    endoplasmic reticulum membrane GO:0005789 IBA
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IEA
    synaptic vesicle GO:0008021 ISS
    ER to Golgi transport vesicle membrane GO:0012507 IBA
    clathrin-coated vesicle GO:0030136 ISS
    synaptic vesicle membrane GO:0030672 IEA
    SNARE complex GO:0031201 IBA
    SNARE complex GO:0031201 ISS
    SNARE complex GO:0031201 TAS
    late endosome membrane GO:0031902 IBA
    trans-Golgi network membrane GO:0032588 TAS
    neuronal cell body GO:0043025 ISS
    intracellular membrane-bounded organelle GO:0043231 IDA
    neuron projection terminus GO:0044306 ISS
    perinuclear region of cytoplasm GO:0048471 ISS
    hippocampal mossy fiber to CA3 synapse GO:0098686 IEA
 Experiment description of studies that identified VTI1A in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for VTI1A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Affinity Capture-MS Homo sapiens
2 SCFD1 23256
Affinity Capture-MS Homo sapiens
3 NSF 4905
Affinity Capture-MS Homo sapiens
4 BET1 10282
Affinity Capture-MS Homo sapiens
5 TMEM9 252839
Affinity Capture-MS Homo sapiens
6 TMBIM6 7009
Two-hybrid Homo sapiens
7 VTI1B 10490
Affinity Capture-MS Homo sapiens
8 TFRC 7037
Affinity Capture-MS Homo sapiens
9 STX12 23673
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 HAUS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 VAMP8 8673
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
12 LAMTOR1 55004
Proximity Label-MS Homo sapiens
13 MARCKS 4082
Proximity Label-MS Homo sapiens
14 LAMP3  
Proximity Label-MS Homo sapiens
15 RAB5A 5868
Proximity Label-MS Homo sapiens
16 SLC39A4 55630
Affinity Capture-MS Homo sapiens
17 NAPA 8775
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 ZDHHC12  
Affinity Capture-MS Homo sapiens
19 B4GALT5 9334
Affinity Capture-MS Homo sapiens
20 Snap29  
Affinity Capture-MS Mus musculus
21 EDEM1  
Affinity Capture-MS Homo sapiens
22 VAMP4 8674
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 VAMP3 9341
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
24 GOLPH3 64083
Affinity Capture-MS Homo sapiens
25 TMEM9B 56674
Affinity Capture-MS Homo sapiens
26 LAMP2 3920
Proximity Label-MS Homo sapiens
27 ARF6 382
Proximity Label-MS Homo sapiens
28 CLEC12A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 TMEM74  
Affinity Capture-MS Homo sapiens
30 STX11 8676
Affinity Capture-MS Homo sapiens
31 RAB4A 5867
Proximity Label-MS Homo sapiens
32 APEX1 328
Affinity Capture-RNA Homo sapiens
33 B3GAT1  
Proximity Label-MS Homo sapiens
34 RAB2A 5862
Proximity Label-MS Homo sapiens
35 SNAP29 9342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 VPS45 11311
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 RAB9A 9367
Proximity Label-MS Homo sapiens
38 IGF2R 3482
Affinity Capture-MS Homo sapiens
39 ASPH 444
Affinity Capture-MS Homo sapiens
40 VAMP5 10791
Affinity Capture-MS Homo sapiens
41 STX8 9482
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
42 NAPG 8774
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 STX7 8417
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
44 STX16 8675
Affinity Capture-MS Homo sapiens
45 RAB5C 5878
Proximity Label-MS Homo sapiens
46 GPRC5B 51704
Affinity Capture-MS Homo sapiens
47 STX10 8677
Affinity Capture-MS Homo sapiens
48 STX18 53407
Affinity Capture-MS Homo sapiens
49 TMEM171  
Affinity Capture-MS Homo sapiens
50 CLCN5 1184
Affinity Capture-MS Homo sapiens
51 TRPC4AP  
Affinity Capture-MS Homo sapiens
52 STX6 10228
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 HLA-C 3107
Affinity Capture-MS Homo sapiens
54 SMPD1 6609
Affinity Capture-MS Homo sapiens
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