Gene description for B4GALT5
Gene name UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5
Gene symbol B4GALT5
Other names/aliases B4Gal-T5
BETA4-GALT-IV
beta4Gal-T5
beta4GalT-V
gt-V
Species Homo sapiens
 Database cross references - B4GALT5
ExoCarta ExoCarta_9334
Entrez Gene 9334
HGNC 928
MIM 604016
UniProt O43286  
 B4GALT5 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for B4GALT5
Molecular Function
    metal ion binding GO:0046872 IEA
    galactosyltransferase activity GO:0008378 TAS
Biological Process
    carbohydrate metabolic process GO:0005975 TAS
    keratan sulfate metabolic process GO:0042339 TAS
    cellular protein metabolic process GO:0044267 TAS
    keratan sulfate biosynthetic process GO:0018146 TAS
    O-glycan processing GO:0016266 TAS
    post-translational protein modification GO:0043687 TAS
    small molecule metabolic process GO:0044281 TAS
    protein O-linked glycosylation GO:0006493 TAS
    protein N-linked glycosylation via asparagine GO:0018279 TAS
    glycosaminoglycan metabolic process GO:0030203 TAS
Subcellular Localization
    integral component of membrane GO:0016021 IEA
    Golgi membrane GO:0000139 TAS
    Golgi cisterna membrane GO:0032580 IEA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified B4GALT5 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for B4GALT5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which B4GALT5 is involved
PathwayEvidenceSource
Keratan sulfate biosynthesis TAS Reactome
N-Glycan antennae elongation TAS Reactome
O-linked glycosylation of mucins TAS Reactome





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