Gene description for B4GALT5
Gene name UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5
Gene symbol B4GALT5
Other names/aliases B4Gal-T5
BETA4-GALT-IV
beta4Gal-T5
beta4GalT-V
gt-V
Species Homo sapiens
 Database cross references - B4GALT5
ExoCarta ExoCarta_9334
Vesiclepedia VP_9334
Entrez Gene 9334
HGNC 928
MIM 604016
UniProt O43286  
 B4GALT5 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for B4GALT5
Molecular Function
    N-acetyllactosamine synthase activity GO:0003945 IEA
    protein binding GO:0005515 IPI
    galactosyltransferase activity GO:0008378 TAS
    UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity GO:0008489 IBA
    UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity GO:0008489 IMP
    metal ion binding GO:0046872 IEA
Biological Process
    carbohydrate metabolic process GO:0005975 IEA
    protein glycosylation GO:0006486 ISS
    glycoprotein biosynthetic process GO:0009101 IBA
    ganglioside biosynthetic process via lactosylceramide GO:0010706 ISS
    O-glycan processing GO:0016266 TAS
    central nervous system neuron axonogenesis GO:0021955 ISS
    central nervous system myelination GO:0022010 ISS
    poly-N-acetyllactosamine biosynthetic process GO:0030311 IEA
    regulation of protein stability GO:0031647 ISS
    positive regulation of embryonic development GO:0040019 ISS
    neuron maturation GO:0042551 ISS
    glycosylation GO:0070085 IBA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    Golgi apparatus GO:0005794 IBA
    Golgi cisterna membrane GO:0032580 IEA
 Experiment description of studies that identified B4GALT5 in exosomes
1
Experiment ID 494
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 497
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 498
MISEV standards
EM
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 191
MISEV standards
Biophysical techniques
Alix|CD81|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for B4GALT5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 CCL3  
Affinity Capture-MS Homo sapiens
3 CSTL1  
Affinity Capture-MS Homo sapiens
4 HS3ST1  
Affinity Capture-MS Homo sapiens
5 IL13RA2 3598
Affinity Capture-MS Homo sapiens
6 TMEM59 9528
Affinity Capture-MS Homo sapiens
7 PCDH20  
Affinity Capture-MS Homo sapiens
8 LIPH 200879
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 VTI1A 143187
Affinity Capture-MS Homo sapiens
10 ATG9A 79065
Affinity Capture-MS Homo sapiens
11 TNF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 NRSN1  
Affinity Capture-MS Homo sapiens
13 FCGRT 2217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 IL20RB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CHST8 64377
Affinity Capture-MS Homo sapiens
16 PMCH  
Affinity Capture-MS Homo sapiens
17 HSPA13 6782
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 NAPA 8775
Affinity Capture-MS Homo sapiens
19 IFNE  
Affinity Capture-MS Homo sapiens
20 ERP27  
Affinity Capture-MS Homo sapiens
21 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
22 TMX1 81542
Affinity Capture-MS Homo sapiens
23 MGAT4C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 GPR114  
Affinity Capture-MS Homo sapiens
25 OMA1  
Affinity Capture-MS Homo sapiens
26 PGLYRP3 114771
Affinity Capture-MS Homo sapiens
27 C16orf92  
Affinity Capture-MS Homo sapiens
28 IL27RA 9466
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 SLC39A9 55334
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 KIAA1467 57613
Affinity Capture-MS Homo sapiens
31 EDDM3A  
Affinity Capture-MS Homo sapiens
32 GABRG2  
Affinity Capture-MS Homo sapiens
33 B4GALT6  
Affinity Capture-MS Homo sapiens
34 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
35 OPTC  
Affinity Capture-MS Homo sapiens
36 IDS  
Affinity Capture-MS Homo sapiens
37 AVP  
Affinity Capture-MS Homo sapiens
38 B3GNT7  
Affinity Capture-MS Homo sapiens
39 CTLA4  
Affinity Capture-MS Homo sapiens
40 NXPH2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 ADIPOQ 9370
Affinity Capture-MS Homo sapiens
42 CRP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 IGF2R 3482
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 GINM1 116254
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 EPO 2056
Affinity Capture-MS Homo sapiens
46 KERA  
Affinity Capture-MS Homo sapiens
47 TPCN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 CSF2 1437
Affinity Capture-MS Homo sapiens
49 SYT12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 HLA-DPA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 CORT  
Affinity Capture-MS Homo sapiens
52 TMPRSS13  
Affinity Capture-MS Homo sapiens
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