Gene description for TMEM59
Gene name transmembrane protein 59
Gene symbol TMEM59
Other names/aliases C1orf8
DCF1
HSPC001
PRO195
UNQ169
Species Homo sapiens
 Database cross references - TMEM59
ExoCarta ExoCarta_9528
Vesiclepedia VP_9528
Entrez Gene 9528
HGNC 1239
UniProt Q9BXS4  
 TMEM59 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Saliva 19199708    
Urine 19056867    
 Gene ontology annotations for TMEM59
Molecular Function
    endopeptidase activity GO:0004175 IEA
    protein binding GO:0005515 IPI
Biological Process
    protein glycosylation GO:0006486 IDA
    autophagy GO:0006914 IEA
    positive regulation of autophagy GO:0010508 IBA
    positive regulation of autophagy GO:0010508 IMP
    negative regulation of protein localization to plasma membrane GO:1903077 IDA
Subcellular Localization
    Golgi cis cisterna GO:0000137 IDA
    Golgi trans cisterna GO:0000138 IDA
    Golgi membrane GO:0000139 IEA
    lysosome GO:0005764 IBA
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 IEA
    late endosome GO:0005770 IBA
    late endosome GO:0005770 IDA
    Golgi medial cisterna GO:0005797 IDA
    plasma membrane GO:0005886 IEA
    late endosome membrane GO:0031902 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified TMEM59 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
10
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for TMEM59
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HMP19  
Affinity Capture-MS Homo sapiens
2 CALHM2 51063
Affinity Capture-MS Homo sapiens
3 POMT2  
Affinity Capture-MS Homo sapiens
4 ST8SIA3  
Affinity Capture-MS Homo sapiens
5 SGCA  
Affinity Capture-MS Homo sapiens
6 FKRP 79147
Affinity Capture-MS Homo sapiens
7 CSGALNACT2 55454
Affinity Capture-MS Homo sapiens
8 FJX1 24147
Affinity Capture-MS Homo sapiens
9 NRSN1  
Affinity Capture-MS Homo sapiens
10 IL20RB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 LPHN1  
Affinity Capture-MS Homo sapiens
12 CFC1  
Affinity Capture-MS Homo sapiens
13 APOE 348
Affinity Capture-MS Homo sapiens
14 LMBR1  
Affinity Capture-MS Homo sapiens
15 TMEM59L  
Affinity Capture-MS Homo sapiens
16 SLC27A6  
Affinity Capture-MS Homo sapiens
17 GPR114  
Affinity Capture-MS Homo sapiens
18 PDE3B  
Affinity Capture-MS Homo sapiens
19 GLCE 26035
Affinity Capture-MS Homo sapiens
20 IL20  
Affinity Capture-MS Homo sapiens
21 CPT1A 1374
Affinity Capture-MS Homo sapiens
22 POMGNT2  
Affinity Capture-MS Homo sapiens
23 MGAT5 4249
Affinity Capture-MS Homo sapiens
24 VANGL2  
Affinity Capture-MS Homo sapiens
25 ABCA3 21
Affinity Capture-MS Homo sapiens
26 QSOX2 169714
Affinity Capture-MS Homo sapiens
27 B3GAT1  
Proximity Label-MS Homo sapiens
28 B3GNT2 10678
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 ADAM9 8754
Affinity Capture-MS Homo sapiens
30 YES1 7525
Affinity Capture-MS Homo sapiens
31 B4GALT7 11285
Affinity Capture-MS Homo sapiens
32 STX8 9482
Affinity Capture-MS Homo sapiens
33 MMP15 4324
Affinity Capture-MS Homo sapiens
34 CUX1 1523
Affinity Capture-MS Homo sapiens
35 FGFR4 2264
Affinity Capture-MS Homo sapiens
36 MGAT1 4245
Affinity Capture-MS Homo sapiens
37 GGA1 26088
Affinity Capture-MS Homo sapiens
38 NMU  
Affinity Capture-MS Homo sapiens
39 TMEM192 201931
Affinity Capture-MS Homo sapiens
40 ABHD14A  
Affinity Capture-MS Homo sapiens
41 TNFSF9  
Affinity Capture-MS Homo sapiens
42 MAN1A1 4121
Affinity Capture-MS Homo sapiens
43 ATG16L1 55054
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
44 LRFN1  
Affinity Capture-MS Homo sapiens
45 B4GALT1 2683
Affinity Capture-MS Homo sapiens
46 EPHX4 253152
Affinity Capture-MS Homo sapiens
47 PRTN3 5657
Affinity Capture-MS Homo sapiens
48 CEP131 22994
Affinity Capture-MS Homo sapiens
49 FGFR1 2260
Affinity Capture-MS Homo sapiens
50 ALG5 29880
Affinity Capture-MS Homo sapiens
51 ZDHHC9  
Affinity Capture-MS Homo sapiens
52 CHST12 55501
Affinity Capture-MS Homo sapiens
53 GOLIM4 27333
Affinity Capture-MS Homo sapiens
54 GLT8D1 55830
Affinity Capture-MS Homo sapiens
55 GJA1 2697
Affinity Capture-MS Homo sapiens
56 TOR1B 27348
Affinity Capture-MS Homo sapiens
57 FASTKD5  
Affinity Capture-MS Homo sapiens
58 ST6GALNAC6 30815
Affinity Capture-MS Homo sapiens
59 CEACAM21  
Affinity Capture-MS Homo sapiens
60 B4GALT6  
Affinity Capture-MS Homo sapiens
61 SCAP  
Affinity Capture-MS Homo sapiens
62 UGT8  
Affinity Capture-MS Homo sapiens
63 HS6ST1  
Affinity Capture-MS Homo sapiens
64 TMED4 222068
Affinity Capture-MS Homo sapiens
65 TMEM43 79188
Affinity Capture-MS Homo sapiens
66 DNAJC5 80331
Proximity Label-MS Homo sapiens
67 CHPT1  
Affinity Capture-MS Homo sapiens
68 SREBF2 6721
Affinity Capture-MS Homo sapiens
69 B4GALT5 9334
Affinity Capture-MS Homo sapiens
70 MAN2A2 4122
Affinity Capture-MS Homo sapiens
71 NTRK1 4914
Affinity Capture-MS Homo sapiens
72 CRP  
Affinity Capture-MS Homo sapiens
73 B3GAT2  
Affinity Capture-MS Homo sapiens
74 TRIM32 22954
Affinity Capture-MS Homo sapiens
75 SLC25A17 10478
Affinity Capture-MS Homo sapiens
76 B4GALT3 8703
Affinity Capture-MS Homo sapiens
77 BMPR1A 657
Affinity Capture-MS Homo sapiens
78 ELOVL5 60481
Proximity Label-MS Homo sapiens
79 CORT  
Affinity Capture-MS Homo sapiens
80 UST  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 UPK2 7379
Affinity Capture-MS Homo sapiens
82 SPPL3  
Affinity Capture-MS Homo sapiens
83 PCDHA8  
Affinity Capture-MS Homo sapiens
84 LAPTM4B  
Affinity Capture-MS Homo sapiens
85 TMEM30A 55754
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 CXCR4 7852
Affinity Capture-MS Homo sapiens
87 CHST8 64377
Affinity Capture-MS Homo sapiens
88 IQCB1  
Affinity Capture-MS Homo sapiens
89 CBLN4  
Affinity Capture-MS Homo sapiens
90 ST8SIA2  
Affinity Capture-MS Homo sapiens
91 PMCH  
Affinity Capture-MS Homo sapiens
92 LPCAT4  
Affinity Capture-MS Homo sapiens
93 SLC26A2 1836
Affinity Capture-MS Homo sapiens
94 IFNE  
Affinity Capture-MS Homo sapiens
95 TMEM246 84302
Affinity Capture-MS Homo sapiens
96 ENTPD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 KIAA1467 57613
Affinity Capture-MS Homo sapiens
98 EXT1 2131
Affinity Capture-MS Homo sapiens
99 ZMYM6  
Affinity Capture-MS Homo sapiens
100 MAN1A2 10905
Affinity Capture-MS Homo sapiens
101 A4GNT  
Affinity Capture-MS Homo sapiens
102 DSE 29940
Affinity Capture-MS Homo sapiens
103 B3GAT3 26229
Affinity Capture-MS Homo sapiens
104 RNF128  
Affinity Capture-MS Homo sapiens
105 GALNT2 2590
Affinity Capture-MS Homo sapiens
106 ISLR 3671
Affinity Capture-MS Homo sapiens
107 ENPP4 22875
Affinity Capture-MS Homo sapiens
108 ATP7A 538
Affinity Capture-MS Homo sapiens
109 SCCPDH 51097
Affinity Capture-MS Homo sapiens
110 FUT1  
Affinity Capture-MS Homo sapiens
111 ATP2B3 492
Affinity Capture-MS Homo sapiens
112 LMBRD2 92255
Affinity Capture-MS Homo sapiens
113 GJC1 10052
Affinity Capture-MS Homo sapiens
114 LRP10 26020
Affinity Capture-MS Homo sapiens
115 GGA3 23163
Affinity Capture-MS Homo sapiens
116 COLEC12 81035
Affinity Capture-MS Homo sapiens
117 GPR89A  
Affinity Capture-MS Homo sapiens
118 ABCB6 10058
Affinity Capture-MS Homo sapiens
119 GOSR1 9527
Affinity Capture-MS Homo sapiens
120 CD1B 910
Affinity Capture-MS Homo sapiens
121 RYK 6259
Affinity Capture-MS Homo sapiens
122 VAMP4 8674
Affinity Capture-MS Homo sapiens
123 TYW1  
Affinity Capture-MS Homo sapiens
124 EFNB1 1947
Affinity Capture-MS Homo sapiens
125 B4GAT1 11041
Affinity Capture-MS Homo sapiens
126 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
127 LRP12 29967
Affinity Capture-MS Homo sapiens
128 TIMP3 7078
Affinity Capture-MS Homo sapiens
129 UGCG 7357
Affinity Capture-MS Homo sapiens
130 FKTN  
Affinity Capture-MS Homo sapiens
131 SFTPC  
Affinity Capture-MS Homo sapiens
132 GPR50  
Affinity Capture-MS Homo sapiens
133 EDDM3A  
Affinity Capture-MS Homo sapiens
134 TNFRSF10B 8795
Affinity Capture-MS Homo sapiens
135 EMILIN2 84034
Affinity Capture-MS Homo sapiens
136 SYNGR3  
Affinity Capture-MS Homo sapiens
137 PTPRS 5802
Affinity Capture-MS Homo sapiens
138 FAM69A 388650
Affinity Capture-MS Homo sapiens
139 ERRFI1 54206
Two-hybrid Homo sapiens
140 UGT1A7 54577
Affinity Capture-MS Homo sapiens
141 KCNT2  
Affinity Capture-MS Homo sapiens
142 POMK  
Affinity Capture-MS Homo sapiens
143 TPST2 8459
Affinity Capture-MS Homo sapiens
144 CUL7 9820
Affinity Capture-MS Homo sapiens
145 GOLM1 51280
Affinity Capture-MS Homo sapiens
146 CHST11 50515
Affinity Capture-MS Homo sapiens
147 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
148 CHSY1 22856
Affinity Capture-MS Homo sapiens
149 HS6ST2 90161
Affinity Capture-MS Homo sapiens
150 HLA-C 3107
Affinity Capture-MS Homo sapiens
151 DKKL1  
Affinity Capture-MS Homo sapiens
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