Gene description for TMEM59
Gene name transmembrane protein 59
Gene symbol TMEM59
Other names/aliases C1orf8
DCF1
HSPC001
PRO195
UNQ169
Species Homo sapiens
 Database cross references - TMEM59
ExoCarta ExoCarta_9528
Entrez Gene 9528
HGNC 1239
UniProt Q9BXS4  
 TMEM59 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Saliva 19199708    
Urine 19056867    
 Gene ontology annotations for TMEM59
Molecular Function
    endopeptidase activity GO:0004175 IEA
    protein binding GO:0005515 IPI
Biological Process
    autophagy GO:0006914 IEA
    negative regulation of establishment of protein localization to plasma membrane GO:0090005 IDA
    proteolysis GO:0006508 IEA
    negative regulation of protein glycosylation in Golgi GO:0090285 IDA
    negative regulation of protein processing GO:0010955 IDA
    positive regulation of autophagy GO:0010508 IMP
Subcellular Localization
    lysosomal membrane GO:0005765 IEA
    Golgi membrane GO:0000139 IEA
    late endosome GO:0005770 IDA
    Golgi cis cisterna GO:0000137 IDA
    Golgi trans cisterna GO:0000138 IDA
    Golgi medial cisterna GO:0005797 IDA
    late endosome membrane GO:0031902 IEA
    extracellular exosome GO:0070062 IDA
    lysosome GO:0005764 IDA
    integral component of membrane GO:0016021 IEA
    plasma membrane GO:0005886 IEA
 Experiment description of studies that identified TMEM59 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 66
ISEV standards
IEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for TMEM59
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which TMEM59 is involved
No pathways found





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