Gene description for B4GALT1
Gene name UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
Gene symbol B4GALT1
Other names/aliases B4GAL-T1
CDG2D
GGTB2
GT1
GTB
beta4Gal-T1
Species Homo sapiens
 Database cross references - B4GALT1
ExoCarta ExoCarta_2683
Entrez Gene 2683
HGNC 924
MIM 137060
UniProt P15291  
 B4GALT1 identified in exosomes derived from the following tissue/cell type
Breast milk 17641064    
Endothelial cells 26027894    
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Saliva 19199708    
Squamous carcinoma cells 20124223    
Urine 19056867    
 Gene ontology annotations for B4GALT1
Molecular Function
    alpha-tubulin binding GO:0043014 IDA
    protein homodimerization activity GO:0042803 IDA
    galactosyltransferase activity GO:0008378 NAS
    beta-tubulin binding GO:0048487 IPI
    lactose synthase activity GO:0004461 IDA
    cytoskeletal protein binding GO:0008092 IEA
    beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity GO:0003831 IDA
    UDP-galactosyltransferase activity GO:0035250 IDA
    manganese ion binding GO:0030145 IDA
    N-acetyllactosamine synthase activity GO:0003945 IDA
Biological Process
    acute inflammatory response GO:0002526 IEA
    extracellular matrix organization GO:0030198 IEA
    Notch signaling pathway GO:0007219 TAS
    multicellular organism reproduction GO:0032504 TAS
    keratan sulfate biosynthetic process GO:0018146 TAS
    branching morphogenesis of an epithelial tube GO:0048754 IEA
    cell adhesion GO:0007155 IEA
    regulation of cellular component movement GO:0051270 IEA
    lactose biosynthetic process GO:0005989 TAS
    binding of sperm to zona pellucida GO:0007339 TAS
    positive regulation of apoptotic process involved in mammary gland involution GO:0060058 IEA
    mammary gland development GO:0030879 IEA
    development of secondary sexual characteristics GO:0045136 IEA
    post-translational protein modification GO:0043687 TAS
    single fertilization GO:0007338 TAS
    angiogenesis involved in wound healing GO:0060055 IEA
    keratan sulfate metabolic process GO:0042339 TAS
    glycosaminoglycan metabolic process GO:0030203 TAS
    cellular protein metabolic process GO:0044267 TAS
    leukocyte migration GO:0050900 IEA
    protein N-linked glycosylation GO:0006487 IDA
    positive regulation of epithelial cell proliferation involved in wound healing GO:0060054 IEA
    carbohydrate metabolic process GO:0005975 TAS
    small molecule metabolic process GO:0044281 TAS
    penetration of zona pellucida GO:0007341 IEA
    negative regulation of cell proliferation GO:0008285 IEA
    protein N-linked glycosylation via asparagine GO:0018279 TAS
    epithelial cell development GO:0002064 IEA
    oligosaccharide biosynthetic process GO:0009312 NAS
    regulation of acrosome reaction GO:0060046 IEA
    galactose metabolic process GO:0006012 IEA
Subcellular Localization
    filopodium GO:0030175 IEA
    Golgi cisterna membrane GO:0032580 IEA
    membrane GO:0016020 IDA
    basolateral plasma membrane GO:0016323 IDA
    external side of plasma membrane GO:0009897 IDA
    Golgi membrane GO:0000139 TAS
    brush border membrane GO:0031526 IDA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 TAS
    extracellular exosome GO:0070062 IDA
    desmosome GO:0030057 IDA
    glycocalyx GO:0030112 IDA
    Golgi trans cisterna GO:0000138 IDA
    extracellular space GO:0005615 IDA
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified B4GALT1 in exosomes
1
Experiment ID 48
ISEV standards
EM|IEM
EV Biophysical techniques
HSC70
EV Cytosolic markers
CD63|CD86|MHCII
EV Membrane markers
CANX
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Mature milk
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
2
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
3
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
4
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 66
ISEV standards
IEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
7
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for B4GALT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDC2L2  
Invitro Homo sapiens
2 LALBA  
Invitro Homo sapiens
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