Gene description for Cdc6
Gene name cell division cycle 6
Gene symbol Cdc6
Other names/aliases CDC18L
Species Mus musculus
 Database cross references - Cdc6
ExoCarta ExoCarta_23834
Vesiclepedia VP_23834
Entrez Gene 23834
UniProt O89033  
 Cdc6 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Cdc6
Molecular Function
    chromatin binding GO:0003682 IDA
    DNA replication origin binding GO:0003688 IBA
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    kinase binding GO:0019900 ISO
Biological Process
    DNA replication initiation GO:0006270 IBA
    DNA replication initiation GO:0006270 IEA
    regulation of mitotic metaphase/anaphase transition GO:0030071 ISO
    positive regulation of cytokinesis GO:0032467 ISO
    mitotic DNA replication checkpoint signaling GO:0033314 IBA
    positive regulation of fibroblast proliferation GO:0048146 ISO
    cell division GO:0051301 IEA
    positive regulation of chromosome segregation GO:0051984 ISO
Subcellular Localization
    spindle pole GO:0000922 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IDA
    spindle GO:0005819 IDA
    cytosol GO:0005829 ISO
    intercellular bridge GO:0045171 ISO
    spindle midzone GO:0051233 ISO
    mitotic spindle GO:0072686 ISO
 Experiment description of studies that identified Cdc6 in exosomes
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Cdc6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BCLAF1 9774
Affinity Capture-MS Homo sapiens
2 B4GALT1 2683
Affinity Capture-MS Homo sapiens
3 PRPF4B 8899
Affinity Capture-MS Homo sapiens
4 AIFM1 9131
Affinity Capture-MS Homo sapiens
5 CLASP2 23122
Affinity Capture-MS Homo sapiens
6 ZNF598 90850
Affinity Capture-MS Homo sapiens
7 TFAP2A  
Affinity Capture-MS Homo sapiens
8 Cdk2  
Affinity Capture-Western Mus musculus
9 UBA52 7311
Affinity Capture-MS Homo sapiens
10 THRAP3 9967
Affinity Capture-MS Homo sapiens
11 CDC6  
Affinity Capture-MS Homo sapiens
12 COX6B1 1340
Affinity Capture-MS Homo sapiens
13 BAIAP2L1 55971
Affinity Capture-MS Homo sapiens
14 Tmem173  
Proximity Label-MS Mus musculus
15 TRIM14 9830
Affinity Capture-MS Homo sapiens
16 USP24 23358
Affinity Capture-MS Homo sapiens
17 KMT2D 8085
Affinity Capture-MS Homo sapiens
18 MST1R 4486
Affinity Capture-MS Homo sapiens
19 ABCE1 6059
Affinity Capture-MS Homo sapiens
20 PDF  
Affinity Capture-MS Homo sapiens
21 Hist2h2bb  
Co-fractionation Mus musculus
22 PTGR2 145482
Affinity Capture-MS Homo sapiens
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