Gene description for TRIM14
Gene name tripartite motif containing 14
Gene symbol TRIM14
Other names/aliases -
Species Homo sapiens
 Database cross references - TRIM14
ExoCarta ExoCarta_9830
Vesiclepedia VP_9830
Entrez Gene 9830
HGNC 16283
MIM 606556
UniProt Q14142  
 TRIM14 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for TRIM14
Molecular Function
    transcription coactivator activity GO:0003713 IDA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    molecular adaptor activity GO:0060090 IDA
    ubiquitin protein ligase activity GO:0061630 IBA
Biological Process
    inflammatory response GO:0006954 IDA
    negative regulation of viral transcription GO:0032897 IDA
    innate immune response GO:0045087 IBA
    innate immune response GO:0045087 IDA
    positive regulation of DNA-templated transcription GO:0045893 IEA
    positive regulation of DNA-binding transcription factor activity GO:0051091 IDA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 IDA
    mitochondrial outer membrane GO:0005741 IEA
    phagocytic vesicle GO:0045335 IEA
 Experiment description of studies that identified TRIM14 in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for TRIM14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAVS 57506
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
2 NCK1 4690
Affinity Capture-MS Homo sapiens
3 PPP4C 5531
Affinity Capture-MS Homo sapiens
4 TRAF2 7186
Two-hybrid Homo sapiens
5 CUL3 8452
Affinity Capture-MS Homo sapiens
6 CCDC36  
Two-hybrid Homo sapiens
7 EXOC8 149371
Two-hybrid Homo sapiens
8 KIFC3 3801
Two-hybrid Homo sapiens
9 FAM178A  
Two-hybrid Homo sapiens
10 GOLGA6L9  
Two-hybrid Homo sapiens
11 CCDC57 284001
Two-hybrid Homo sapiens
12 POLR1C 9533
Two-hybrid Homo sapiens
13 MYO15B 80022
Two-hybrid Homo sapiens
14 IKBKG 8517
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
15 Oxnad1  
Affinity Capture-MS Mus musculus
16 BYSL 705
Two-hybrid Homo sapiens
17 ERCC3  
Two-hybrid Homo sapiens
18 CCDC125 202243
Two-hybrid Homo sapiens
19 SAMD1  
Affinity Capture-MS Homo sapiens
20 FAM50B  
Two-hybrid Homo sapiens
21 CKS1B  
Two-hybrid Homo sapiens
22 TRAF3 7187
Affinity Capture-Western Homo sapiens
23 GOLGA2 2801
Two-hybrid Homo sapiens
24 RAD51D  
Two-hybrid Homo sapiens
25 TBK1 29110
Affinity Capture-Western Homo sapiens
26 ADAMTS12 81792
Two-hybrid Homo sapiens
27 CARD9 64170
Two-hybrid Homo sapiens
28 HEXIM2  
Two-hybrid Homo sapiens
29 HNRNPF 3185
Two-hybrid Homo sapiens
30 Cdc6 23834
Affinity Capture-MS Mus musculus
31 CIB3  
Two-hybrid Homo sapiens
32 RNF2  
Affinity Capture-MS Homo sapiens
33 ZGPAT  
Two-hybrid Homo sapiens
34 BNC1  
Affinity Capture-MS Homo sapiens
35 PRKAB2 5565
Two-hybrid Homo sapiens
36 CCDC6 8030
Two-hybrid Homo sapiens
37 PRPF31 26121
Two-hybrid Homo sapiens
38 TSGA10  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which TRIM14 is involved
PathwayEvidenceSource
Cytokine Signaling in Immune system TAS Reactome
Immune System TAS Reactome
Interferon gamma signaling TAS Reactome
Interferon Signaling TAS Reactome





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