Gene description for POLR1C
Gene name polymerase (RNA) I polypeptide C, 30kDa
Gene symbol POLR1C
Other names/aliases RPA39
RPA40
RPA5
RPAC1
TCS3
Species Homo sapiens
 Database cross references - POLR1C
ExoCarta ExoCarta_9533
Entrez Gene 9533
HGNC 20194
MIM 610060
UniProt O15160  
 POLR1C identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for POLR1C
Molecular Function
    RNA polymerase III activity GO:0001056 IBA
    protein dimerization activity GO:0046983 IEA
    RNA polymerase I activity GO:0001054 IBA
    DNA binding GO:0003677 IEA
    protein binding GO:0005515 IPI
    DNA-directed RNA polymerase activity GO:0003899 TAS
Biological Process
    regulation of gene expression, epigenetic GO:0040029 TAS
    positive regulation of type I interferon production GO:0032481 TAS
    transcription initiation from RNA polymerase I promoter GO:0006361 TAS
    termination of RNA polymerase III transcription GO:0006386 TAS
    transcription elongation from RNA polymerase III promoter GO:0006385 TAS
    transcription elongation from RNA polymerase I promoter GO:0006362 TAS
    transcription from RNA polymerase I promoter GO:0006360 TAS
    termination of RNA polymerase I transcription GO:0006363 TAS
    gene expression GO:0010467 TAS
    innate immune response GO:0045087 TAS
    negative regulation of gene expression, epigenetic GO:0045814 TAS
    transcription from RNA polymerase III promoter GO:0006383 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    nucleoplasm GO:0005654 TAS
    DNA-directed RNA polymerase III complex GO:0005666 IDA
    DNA-directed RNA polymerase I complex GO:0005736 TAS
 Experiment description of studies that identified POLR1C in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for POLR1C
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Co-fractionation Homo sapiens
Far Western Homo sapiens
2 POLR1D 51082
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 PAF53  
Affinity Capture-MS Homo sapiens
Far Western Homo sapiens
Co-purification Homo sapiens
4 NME1 4830
Two-hybrid Homo sapiens
5 WRN  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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