Gene description for POLR3B
Gene name polymerase (RNA) III (DNA directed) polypeptide B
Gene symbol POLR3B
Other names/aliases C128
HLD8
INMAP
RPC2
Species Homo sapiens
 Database cross references - POLR3B
ExoCarta ExoCarta_55703
Entrez Gene 55703
HGNC 30348
MIM 614366
UniProt Q9NW08  
 POLR3B identified in exosomes derived from the following tissue/cell type
Prostate cancer cells 25844599    
Thymus 23844026    
 Gene ontology annotations for POLR3B
Molecular Function
    DNA binding GO:0003677 IEA
    metal ion binding GO:0046872 IEA
    ribonucleoside binding GO:0032549 IEA
    RNA polymerase III activity GO:0001056 IBA
Biological Process
    gene expression GO:0010467 TAS
    transcription elongation from RNA polymerase III promoter GO:0006385 TAS
    positive regulation of type I interferon production GO:0032481 TAS
    innate immune response GO:0045087 TAS
    positive regulation of interferon-beta production GO:0032728 IMP
    defense response to virus GO:0051607 IEA
    transcription from RNA polymerase III promoter GO:0006383 TAS
    termination of RNA polymerase III transcription GO:0006386 TAS
    positive regulation of innate immune response GO:0045089 IMP
Subcellular Localization
    DNA-directed RNA polymerase III complex GO:0005666 IDA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified POLR3B in exosomes
1
Experiment ID 274
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
2
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for POLR3B
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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