Gene description for POLR1A
Gene name polymerase (RNA) I polypeptide A, 194kDa
Gene symbol POLR1A
Other names/aliases A190
RPA1
RPA194
RPO1-4
RPO14
Species Homo sapiens
 Database cross references - POLR1A
ExoCarta ExoCarta_25885
Vesiclepedia VP_25885
Entrez Gene 25885
HGNC 17264
UniProt O95602  
 POLR1A identified in sEVs derived from the following tissue/cell type
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
 Gene ontology annotations for POLR1A
Molecular Function
    magnesium ion binding GO:0000287 IDA
    RNA polymerase I activity GO:0001054 IMP
    RNA polymerase II activity GO:0001055 IEA
    RNA polymerase III activity GO:0001056 IEA
    RNA polymerase IV activity GO:0001057 IEA
    RNA polymerase V activity GO:0001058 IEA
    mitochondrial single subunit type RNA polymerase activity GO:0001065 IEA
    plastid single subunit type RNA polymerase activity GO:0001066 IEA
    DNA binding GO:0003677 IEA
    chromatin binding GO:0003682 IDA
    DNA-directed 5'-3' RNA polymerase activity GO:0003899 IBA
    DNA-directed 5'-3' RNA polymerase activity GO:0003899 IDA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IDA
    DNA/RNA hybrid binding GO:0071667 IDA
Biological Process
    transcription by RNA polymerase IV GO:0001059 IEA
    transcription by RNA polymerase V GO:0001060 IEA
    transcription by RNA polymerase I GO:0006360 IEA
    transcription by RNA polymerase II GO:0006366 IEA
    transcription by RNA polymerase III GO:0006383 IEA
    mitochondrial transcription GO:0006390 IEA
    plastid transcription GO:0042793 IEA
    negative regulation of protein localization to nucleolus GO:1904750 IMP
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    chromosome GO:0005694 ISS
    RNA polymerase I complex GO:0005736 IBA
    RNA polymerase I complex GO:0005736 IDA
 Experiment description of studies that identified POLR1A in sEVs
1
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for POLR1A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 POLR2B 5431
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
2 POLR2G 5436
Affinity Capture-MS Homo sapiens
3 SLFN11 91607
Affinity Capture-MS Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 SMC3 9126
Co-fractionation Homo sapiens
6 XIAP  
Co-fractionation Homo sapiens
7 NOP58 51602
Affinity Capture-Western Homo sapiens
8 LGR4 55366
Affinity Capture-MS Homo sapiens
9 RRN3  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 DBNL 28988
Co-fractionation Homo sapiens
11 NCKAP5L  
Affinity Capture-MS Homo sapiens
12 CDH5  
Affinity Capture-MS Homo sapiens
13 REL 5966
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
14 BRCA1 672
Affinity Capture-Western Homo sapiens
15 CSNK2A1 1457
Affinity Capture-Western Homo sapiens
16 KPNA2 3838
Co-fractionation Homo sapiens
17 SNX21  
Affinity Capture-MS Homo sapiens
18 COPA 1314
Co-fractionation Homo sapiens
19 FAXC  
Affinity Capture-MS Homo sapiens
20 EEF1A2 1917
Co-fractionation Homo sapiens
21 URI1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 USP36  
Affinity Capture-MS Homo sapiens
23 CAPZB 832
Affinity Capture-MS Homo sapiens
24 RPL10A 4736
Co-fractionation Homo sapiens
25 RUVBL1 8607
Affinity Capture-MS Homo sapiens
26 WDR76  
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
27 NOLC1 9221
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
28 DIAPH2  
Affinity Capture-MS Homo sapiens
29 COPB2 9276
Co-fractionation Homo sapiens
30 POLR2I 5438
Affinity Capture-MS Homo sapiens
31 PLCH1  
Co-fractionation Homo sapiens
32 FBXW7  
Affinity Capture-MS Homo sapiens
33 TWISTNB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
34 POLR1E  
Far Western Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
35 MAD2L2 10459
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 CRCP  
Affinity Capture-MS Homo sapiens
37 TEX19  
Affinity Capture-MS Homo sapiens
38 ATG16L1 55054
Affinity Capture-MS Homo sapiens
39 POLR2C 5432
Affinity Capture-MS Homo sapiens
40 GPN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 CUL1 8454
Co-fractionation Homo sapiens
42 POLR2M  
Affinity Capture-MS Homo sapiens
43 PFDN6 10471
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 MTIF2 4528
Co-fractionation Homo sapiens
45 OBSL1 23363
Affinity Capture-MS Homo sapiens
46 GPN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 POLR2K  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 TCOF1 6949
Affinity Capture-Western Homo sapiens
49 TCP1 6950
Cross-Linking-MS (XL-MS) Homo sapiens
50 SHPRH  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
51 UXT 8409
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 RPL9 6133
Co-fractionation Homo sapiens
53 USP11 8237
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 POLR3K  
Affinity Capture-MS Homo sapiens
55 COPE 11316
Co-fractionation Homo sapiens
56 HSP90B1 7184
Co-fractionation Homo sapiens
57 SUPT16H 11198
Affinity Capture-MS Homo sapiens
58 LGALS13  
Affinity Capture-MS Homo sapiens
59 PFDN2 5202
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 LRPPRC 10128
Co-fractionation Homo sapiens
61 TRIP10 9322
Co-fractionation Homo sapiens
62 KIAA1522 57648
Affinity Capture-MS Homo sapiens
63 ENO1 2023
Affinity Capture-RNA Homo sapiens
64 SMC1A 8243
Co-fractionation Homo sapiens
65 FBLN5 10516
Affinity Capture-MS Homo sapiens
66 HECTD1 25831
Affinity Capture-MS Homo sapiens
67 NFKB2 4791
Co-fractionation Homo sapiens
68 MYBBP1A 10514
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
69 IQGAP2 10788
Co-fractionation Homo sapiens
70 TAF13  
Affinity Capture-MS Homo sapiens
71 CD3EAP  
Far Western Homo sapiens
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 SCARNA22  
Affinity Capture-RNA Homo sapiens
73 ARRB1 408
Affinity Capture-MS Homo sapiens
74 RECQL5  
Affinity Capture-Western Homo sapiens
75 POLR3A 11128
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
76 POLR2H 5437
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
77 ECD  
Affinity Capture-MS Homo sapiens
78 SMARCA5 8467
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
79 SIRT7  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
80 TRIM10  
Affinity Capture-MS Homo sapiens
81 POLR2E 5434
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
82 TAF1B  
Affinity Capture-MS Homo sapiens
83 CD6 923
Affinity Capture-MS Homo sapiens
84 RPRD2  
Affinity Capture-MS Homo sapiens
85 RPRD1A  
Affinity Capture-MS Homo sapiens
86 POLR2D  
Affinity Capture-MS Homo sapiens
87 BCCIP 56647
Co-fractionation Homo sapiens
88 POLR2A 5430
Affinity Capture-MS Homo sapiens
89 POLR3E 55718
Affinity Capture-MS Homo sapiens
90 TAF1C  
Affinity Capture-MS Homo sapiens
91 NXF1 10482
Affinity Capture-RNA Homo sapiens
92 CRYBB3  
Affinity Capture-MS Homo sapiens
93 MAPK6  
Affinity Capture-MS Homo sapiens
94 RPA3 6119
Proximity Label-MS Homo sapiens
95 INO80  
Co-fractionation Homo sapiens
96 FAM46A  
Affinity Capture-MS Homo sapiens
97 RPAP3 79657
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 NFKB1 4790
Co-fractionation Homo sapiens
99 UBTF 7343
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
100 DDRGK1 65992
Affinity Capture-MS Homo sapiens
101 RNF2  
Affinity Capture-MS Homo sapiens
102 WDR92 116143
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 L1TD1  
Affinity Capture-MS Homo sapiens
104 C9orf72  
Affinity Capture-MS Homo sapiens
105 CCDC8  
Affinity Capture-MS Homo sapiens
106 EEF1A1 1915
Co-fractionation Homo sapiens
107 RELA 5970
Co-fractionation Homo sapiens
108 ARRB2 409
Affinity Capture-MS Homo sapiens
109 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
110 FBXW11  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
111 POLR2F  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 SIRT6  
Affinity Capture-MS Homo sapiens
113 PARP1 142
Proximity Label-MS Homo sapiens
114 POLR2J  
Affinity Capture-MS Homo sapiens
115 POLR1B 84172
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
116 RUVBL2 10856
Affinity Capture-MS Homo sapiens
117 POLR2L 5441
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 TRIM52  
Affinity Capture-MS Homo sapiens
119 POLR3C 10623
Co-fractionation Homo sapiens
120 POLR1C 9533
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
121 KAT2B  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
122 SCP2 6342
Affinity Capture-MS Homo sapiens
123 GFOD1  
Affinity Capture-MS Homo sapiens
124 SKP1 6500
Co-fractionation Homo sapiens
125 RPAP2  
Affinity Capture-MS Homo sapiens
126 VTA1 51534
Co-fractionation Homo sapiens
127 SHMT2 6472
Affinity Capture-RNA Homo sapiens
128 TAF1D  
Affinity Capture-MS Homo sapiens
129 RPS5 6193
Co-fractionation Homo sapiens
130 SKAP1 8631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
131 ERCC6  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
132 TCF4  
Two-hybrid Homo sapiens
133 MOV10 4343
Affinity Capture-RNA Homo sapiens
134 SUPT6H 6830
Affinity Capture-MS Homo sapiens
135 LOC554223  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
136 POLR3D  
Affinity Capture-MS Homo sapiens
137 POLR3B 55703
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
138 RPRD1B 58490
Affinity Capture-MS Homo sapiens
139 PIH1D1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
140 ZNRD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
141 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
142 POLR1D 51082
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
143 POLR3H  
Affinity Capture-MS Homo sapiens
144 NFKBIB  
Co-fractionation Homo sapiens
145 SSRP1 6749
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
146 IKBKB 3551
Co-fractionation Homo sapiens
147 KBTBD8  
Affinity Capture-Western Homo sapiens
148 PDRG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
149 ASB7  
Affinity Capture-MS Homo sapiens
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