Gene description for SMC3
Gene name structural maintenance of chromosomes 3
Gene symbol SMC3
Other names/aliases BAM
BMH
CDLS3
CSPG6
HCAP
SMC3L1
Species Homo sapiens
 Database cross references - SMC3
ExoCarta ExoCarta_9126
Entrez Gene 9126
HGNC 2468
MIM 606062
UniProt Q9UQE7  
 SMC3 identified in exosomes derived from the following tissue/cell type
Malignant pleural effusions 14975938    
Malignant pleural effusions 14975938    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for SMC3
Molecular Function
    mediator complex binding GO:0036033 IEA
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
    chromatin binding GO:0003682 IEA
    protein heterodimerization activity GO:0046982 IPI
    dynein binding GO:0045502 IDA
    microtubule motor activity GO:0003777 NAS
Biological Process
    stem cell maintenance GO:0019827 IEA
    cellular protein metabolic process GO:0044267 TAS
    post-translational protein modification GO:0043687 TAS
    DNA repair GO:0006281 IEA
    protein sumoylation GO:0016925 TAS
    regulation of DNA replication GO:0006275 IMP
    mitotic cell cycle GO:0000278 TAS
    sister chromatid cohesion GO:0007062 NAS
    mitotic nuclear division GO:0007067 TAS
    cell division GO:0051301 IEA
    meiotic nuclear division GO:0007126 IDA
    signal transduction GO:0007165 IDA
    mitotic spindle organization GO:0007052 IEP
    negative regulation of DNA endoreduplication GO:0032876 IMP
    mitotic sister chromatid cohesion GO:0007064 IEA
Subcellular Localization
    basement membrane GO:0005604 TAS
    cohesin complex GO:0008278 NAS
    spindle pole GO:0000922 IDA
    chromosome, centromeric region GO:0000775 TAS
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    lateral element GO:0000800 IEA
    chromosome GO:0005694 TAS
    meiotic cohesin complex GO:0030893 IDA
    cytosol GO:0005829 TAS
    nuclear meiotic cohesin complex GO:0034991 IEA
    chromatin GO:0000785 IDA
    cohesin core heterodimer GO:0008280 IEA
    nucleus GO:0005634 TAS
    nuclear matrix GO:0016363 IDA
 Experiment description of studies that identified SMC3 in exosomes
1
Experiment ID 51
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Breast cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
2
Experiment ID 52
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Lung cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
3
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SMC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CASP4  
Affinity Capture-MS Homo sapiens
2 MAGED1  
Affinity Capture-MS Homo sapiens
3 STAG1  
Co-purification Homo sapiens
4 SMC1A 8243
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 NUMA1 4926
Affinity Capture-Western Homo sapiens
6 SYCP3  
Affinity Capture-Western Homo sapiens
7 REC8L1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 HDAC2 3066
Co-purification Homo sapiens
9 MXD1  
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
10 CDK4 1019
Affinity Capture-MS Homo sapiens
11 MXI1 4601
Invitro Homo sapiens
12 KIFAP3  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
13 TUBB  
Affinity Capture-Western Homo sapiens
14 NEK6 10783
Affinity Capture-MS Homo sapiens
15 SMARCA5 8467
Co-purification Homo sapiens
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