Gene ontology annotations for TMEM165 |
|
Experiment description of studies that identified TMEM165 in exosomes |
1 |
Experiment ID |
207 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP70
|
EV Cytosolic markers |
✔
FLOT1
|
EV Membrane markers |
✔
VDAC
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23161513
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
Authors |
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. |
Journal name |
Mol Cell Proteomics
|
Publication year |
2012 |
Sample |
Colorectal cancer cells |
Sample name |
DKO-1 |
Isolation/purification methods |
Differential centrifugation Filtration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
2 |
Experiment ID |
208 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP70
|
EV Cytosolic markers |
✔
FLOT1
|
EV Membrane markers |
✔
VDAC
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23161513
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
Authors |
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. |
Journal name |
Mol Cell Proteomics
|
Publication year |
2012 |
Sample |
Colorectal cancer cells |
Sample name |
Dks-8 |
Isolation/purification methods |
Differential centrifugation Filtration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
3 |
Experiment ID |
209 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
TSG101|HSP70
|
EV Cytosolic markers |
✔
FLOT1
|
EV Membrane markers |
✔
VDAC
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23161513
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
Authors |
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. |
Journal name |
Mol Cell Proteomics
|
Publication year |
2012 |
Sample |
Colorectal cancer cells |
Sample name |
DLD-1 |
Isolation/purification methods |
Differential centrifugation Filtration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
4 |
Experiment ID |
211 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|Alix
|
EV Cytosolic markers |
✔
EpCAM|TFRC
|
EV Membrane markers |
✔
cytochrome c|GOLGA2
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23333927
|
Organism |
Homo sapiens |
Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
Authors |
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. |
Journal name |
J Proteomics
|
Publication year |
2013 |
Sample |
Ovarian cancer cells |
Sample name |
IGROV1 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.09-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
5 |
Experiment ID |
212 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
TSG101|Alix
|
EV Cytosolic markers |
✔
EpCAM|TFRC
|
EV Membrane markers |
✔
Cytochrome C|GOLGA2
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23333927
|
Organism |
Homo sapiens |
Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
Authors |
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. |
Journal name |
J Proteomics
|
Publication year |
2013 |
Sample |
Ovarian cancer cells |
Sample name |
OVCAR-3 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.09-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
6 |
Experiment ID |
191 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
Alix
|
EV Cytosolic markers |
✔
CD81|CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
20124223
|
Organism |
Homo sapiens |
Experiment description |
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes. |
Authors |
Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK. |
Journal name |
Mol Cell Proteomics
|
Publication year |
2010 |
Sample |
Squamous carcinoma cells |
Sample name |
Squamous carcinoma cell (A431) |
Isolation/purification methods |
Differential centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
Protein-protein interactions for TMEM165 |
|
Protein Interactor |
ExoCarta ID |
Identification method |
PubMed |
Species |
No interactions are found.
|
|
Pathways in which TMEM165 is involved |
No pathways found
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