Gene description for Tubg1
Gene name tubulin, gamma 1
Gene symbol Tubg1
Other names/aliases 1500010O08Rik
AI451582
AI503389
Tubg
Species Mus musculus
 Database cross references - Tubg1
ExoCarta ExoCarta_103733
Vesiclepedia VP_103733
Entrez Gene 103733
UniProt P83887  
 Tubg1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Tubg1
Molecular Function
    structural constituent of cytoskeleton GO:0005200 TAS
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    microtubule nucleator activity GO:0140490 IBA
Biological Process
    mitotic sister chromatid segregation GO:0000070 IBA
    meiotic spindle organization GO:0000212 IBA
    meiotic spindle organization GO:0000212 IEP
    mitotic cell cycle GO:0000278 IBA
    microtubule nucleation GO:0007020 IBA
    microtubule nucleation GO:0007020 TAS
    mitotic spindle organization GO:0007052 IBA
    mitotic spindle organization GO:0007052 TAS
    regulation of mitotic nuclear division GO:0007088 TAS
    cytoplasmic microtubule organization GO:0031122 IEA
Subcellular Localization
    pericentriolar material GO:0000242 IDA
    condensed nuclear chromosome GO:0000794 IDA
    spindle pole GO:0000922 TAS
    gamma-tubulin complex GO:0000930 IEA
    gamma-tubulin complex GO:0000930 ISO
    gamma-tubulin ring complex GO:0000931 IBA
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    centrosome GO:0005813 IBA
    centrosome GO:0005813 IDA
    centrosome GO:0005813 ISO
    centrosome GO:0005813 ISS
    centriole GO:0005814 IDA
    microtubule organizing center GO:0005815 TAS
    spindle GO:0005819 IBA
    polar microtubule GO:0005827 ISO
    polar microtubule GO:0005827 ISS
    spindle microtubule GO:0005876 IDA
    cytoplasmic microtubule GO:0005881 IDA
    cilium GO:0005929 IDA
    cell leading edge GO:0031252 IDA
    ciliary basal body GO:0036064 IDA
    neuron projection GO:0043005 IDA
    apical part of cell GO:0045177 IDA
    recycling endosome GO:0055037 IEA
    recycling endosome GO:0055037 ISO
    non-motile cilium GO:0097730 IDA
    mitotic spindle microtubule GO:1990498 ISO
    mitotic spindle microtubule GO:1990498 ISS
 Experiment description of studies that identified Tubg1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Tubg1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TUBGCP3 10426
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 SLC25A13 10165
Affinity Capture-MS Homo sapiens
3 FMNL1 752
Affinity Capture-MS Homo sapiens
4 NCBP1 4686
Affinity Capture-MS Homo sapiens
5 CCT3 7203
Affinity Capture-MS Homo sapiens
6 CARKD 55739
Affinity Capture-MS Homo sapiens
7 FMR1 2332
Affinity Capture-MS Homo sapiens
8 AFG3L2 10939
Affinity Capture-MS Homo sapiens
9 C4BPA 722
Affinity Capture-MS Homo sapiens
10 SLC25A1 6576
Affinity Capture-MS Homo sapiens
11 ARCN1 372
Affinity Capture-MS Homo sapiens
12 NDUFA4 4697
Affinity Capture-MS Homo sapiens
13 COPA 1314
Affinity Capture-MS Homo sapiens
14 KIF1B 23095
Affinity Capture-MS Homo sapiens
15 GCN1L1 10985
Affinity Capture-MS Homo sapiens
16 PNKD  
Affinity Capture-MS Homo sapiens
17 LGALS3BP 3959
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 NEDD1 121441
Affinity Capture-MS Homo sapiens
19 ATAD3A 55210
Affinity Capture-MS Homo sapiens
20 B4GALT4 8702
Affinity Capture-MS Homo sapiens
21 PC 5091
Affinity Capture-MS Homo sapiens
22 NME7 29922
Affinity Capture-MS Homo sapiens
23 PTPMT1 114971
Affinity Capture-MS Homo sapiens
24 ELOVL1 64834
Affinity Capture-MS Homo sapiens
25 Lmo2  
Two-hybrid Mus musculus
26 Pacsin2 23970
Affinity Capture-Western Mus musculus
27 TUBGCP6 85378
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 SPATA5L1 79029
Affinity Capture-MS Homo sapiens
29 TIMM50 92609
Affinity Capture-MS Homo sapiens
30 SSR3 6747
Affinity Capture-MS Homo sapiens
31 TUBB6 84617
Affinity Capture-MS Homo sapiens
32 CPS1 1373
Affinity Capture-MS Homo sapiens
33 GEMIN2 8487
Affinity Capture-MS Homo sapiens
34 MZT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 CCT2 10576
Affinity Capture-MS Homo sapiens
36 RBMX 27316
Affinity Capture-MS Homo sapiens
37 PFKM 5213
Affinity Capture-MS Homo sapiens
38 HN1L 90861
Affinity Capture-MS Homo sapiens
39 NSUN2 54888
Affinity Capture-MS Homo sapiens
40 TCP1 6950
Affinity Capture-MS Homo sapiens
41 NCBP2 22916
Affinity Capture-MS Homo sapiens
42 SUCLG1 8802
Affinity Capture-MS Homo sapiens
43 TMEM165 55858
Affinity Capture-MS Homo sapiens
44 NHS 4810
Affinity Capture-MS Homo sapiens
45 TUBGCP4 27229
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 CCT5 22948
Affinity Capture-MS Homo sapiens
47 Pacsin3 80708
Affinity Capture-Western Mus musculus
48 COPB1 1315
Affinity Capture-MS Homo sapiens
49 SRRT 51593
Affinity Capture-MS Homo sapiens
50 BDP1  
Affinity Capture-MS Homo sapiens
51 SLC25A22  
Affinity Capture-MS Homo sapiens
52 DNAJA1 3301
Affinity Capture-MS Homo sapiens
53 Fancd2  
Affinity Capture-MS Mus musculus
54 MZT2A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 VAV2 7410
Affinity Capture-MS Homo sapiens
56 FAM127A  
Affinity Capture-MS Homo sapiens
57 PRSS21  
Affinity Capture-MS Homo sapiens
58 COPS7B 64708
Affinity Capture-MS Homo sapiens
59 SDF4 51150
Affinity Capture-MS Homo sapiens
60 SLC25A5 292
Affinity Capture-MS Homo sapiens
61 OXA1L  
Affinity Capture-MS Homo sapiens
62 RBBP6 5930
Affinity Capture-MS Homo sapiens
63 PKMYT1  
Affinity Capture-MS Homo sapiens
64 Pacsin1  
Affinity Capture-Western Mus musculus
65 AIFM1 9131
Affinity Capture-MS Homo sapiens
66 TUBGCP5 114791
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 USP15 9958
Affinity Capture-MS Homo sapiens
68 BAG2 9532
Affinity Capture-MS Homo sapiens
69 Kcnma1  
Affinity Capture-MS Mus musculus
70 CCT6A 908
Affinity Capture-MS Homo sapiens
71 PON2 5445
Affinity Capture-MS Homo sapiens
72 MZT2B 80097
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 SF1 7536
Affinity Capture-MS Homo sapiens
74 TUBB4B 10383
Affinity Capture-MS Homo sapiens
75 CCT4 10575
Affinity Capture-MS Homo sapiens
76 ATXN10 25814
Affinity Capture-MS Homo sapiens
77 BBX 56987
Affinity Capture-MS Homo sapiens
78 LAMA1 284217
Affinity Capture-MS Homo sapiens
79 FXR2 9513
Affinity Capture-MS Homo sapiens
80 ZC3H18  
Affinity Capture-MS Homo sapiens
81 SRSF10 10772
Affinity Capture-MS Homo sapiens
82 RANBP1 5902
Affinity Capture-MS Homo sapiens
83 Eed  
Affinity Capture-MS Mus musculus
84 GGCX 2677
Affinity Capture-MS Homo sapiens
85 SRSF8 10929
Affinity Capture-MS Homo sapiens
86 PRICKLE3  
Affinity Capture-MS Homo sapiens
87 GLUD1 2746
Affinity Capture-MS Homo sapiens
88 FASN 2194
Affinity Capture-MS Homo sapiens
89 ALDH3A2 224
Affinity Capture-MS Homo sapiens
90 TUBGCP2 10844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 CCT8 10694
Affinity Capture-MS Homo sapiens
92 TUBG1 7283
Affinity Capture-MS Homo sapiens
93 DNAJA2 10294
Affinity Capture-MS Homo sapiens
94 FXR1 8087
Affinity Capture-MS Homo sapiens
95 ALDH1B1 219
Affinity Capture-MS Homo sapiens
96 PDCD6 10016
Affinity Capture-MS Homo sapiens
97 KIAA1804  
Affinity Capture-MS Homo sapiens
98 URB1 9875
Affinity Capture-MS Homo sapiens
99 CCT7 10574
Affinity Capture-MS Homo sapiens
100 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
101 Rock2  
Co-fractionation Mus musculus
102 IPO4 79711
Affinity Capture-MS Homo sapiens
103 STXBP2 6813
Affinity Capture-MS Homo sapiens
104 ATP1A1 476
Affinity Capture-MS Homo sapiens
105 FOXM1  
Affinity Capture-MS Homo sapiens
106 C1QBP 708
Affinity Capture-MS Homo sapiens
107 DYNC2H1 79659
Affinity Capture-MS Homo sapiens
108 RBCK1  
Affinity Capture-MS Homo sapiens
109 ATG3 64422
Affinity Capture-MS Homo sapiens
110 Socs1  
Co-fractionation Mus musculus
View the network image/svg+xml



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