Gene description for Pacsin2
Gene name protein kinase C and casein kinase substrate in neurons 2
Gene symbol Pacsin2
Other names/aliases AI197433
Species Mus musculus
 Database cross references - Pacsin2
ExoCarta ExoCarta_23970
Entrez Gene 23970
UniProt Q9WVE8  
 Pacsin2 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
 Gene ontology annotations for Pacsin2
Molecular Function
    cytoskeletal protein binding GO:0008092 IDA
    phospholipid binding GO:0005543 IBA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 ISO
    phosphatidic acid binding GO:0070300 ISO
    lipid binding GO:0008289 IDA
Biological Process
    caveola assembly GO:0070836 ISO
    endocytosis GO:0006897 IEA
    cytoskeleton organization GO:0007010 IBA
    cell projection morphogenesis GO:0048858 IMP
    actin cytoskeleton organization GO:0030036 IEA
    protein localization to endosome GO:0036010 ISO
    signal transduction GO:0007165 TAS
    caveolin-mediated endocytosis GO:0072584 ISO
    membrane tubulation GO:0097320 ISO
    negative regulation of endocytosis GO:0045806 IDA
Subcellular Localization
    trans-Golgi network GO:0005802 IDA
    membrane GO:0016020 IEA
    cytoplasm GO:0005737 ISO
    focal adhesion GO:0005925 ISO
    membrane-bounded vesicle GO:0031988 IBA
    plasma membrane GO:0005886 IEA
    extracellular exosome GO:0070062 ISO
    cytoskeleton GO:0005856 IEA
    intracellular membrane-bounded organelle GO:0043231 ISO
    cell-cell junction GO:0005911 IDA
    recycling endosome membrane GO:0055038 ISO
    nucleoplasm GO:0005654 ISO
    caveola GO:0005901 ISO
    cytoplasmic vesicle GO:0031410 IEA
    cytosol GO:0005829 IDA
    cell projection GO:0042995 IEA
    endosome GO:0005768 IBA
    extrinsic component of membrane GO:0019898 IDA
 Experiment description of studies that identified Pacsin2 in exosomes
1
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Pacsin2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Pacsin2 is involved
No pathways found





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