Gene description for Tuba1a
Gene name tubulin, alpha 1A
Gene symbol Tuba1a
Other names/aliases Tuba-1
Tuba1
Species Mus musculus
 Database cross references - Tuba1a
ExoCarta ExoCarta_22142
Entrez Gene 22142
UniProt P68369  
 Tuba1a identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
Mov neuroglial cells 15210972    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
 Gene ontology annotations for Tuba1a
Molecular Function
    GTP binding GO:0005525 IEA
    structural constituent of cytoskeleton GO:0005200 IEA
    nucleotide binding GO:0000166 IEA
    GTPase activity GO:0003924 IEA
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 ISO
Biological Process
    microtubule-based process GO:0007017 IEA
    protein polymerization GO:0051258 IEA
Subcellular Localization
    protein complex GO:0043234 IEA
    microtubule GO:0005874 ISO
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    recycling endosome GO:0055037 ISO
    extracellular exosome GO:0070062 ISO
    myelin sheath GO:0043209 ISO
    cytoplasm GO:0005737 IEA
    cytoplasmic microtubule GO:0005881 IDA
    cytoskeleton GO:0005856 IEA
    nucleus GO:0005634 ISO
 Experiment description of studies that identified Tuba1a in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 39
ISEV standards
IEM
EV Biophysical techniques
TSG101|HSC70
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 33
ISEV standards
EM|IEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Tuba1a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ACTN2 88
Two-hybrid Homo sapiens
2 MAGED1  
Two-hybrid Homo sapiens
3 BCAR1 9564
Two-hybrid Homo sapiens
4 GNAI2 2771
Two-hybrid Homo sapiens
5 Hsph1 15505
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
6 TM4SF1 4071
Two-hybrid Homo sapiens
7 S100A8 6279
Two-hybrid Homo sapiens
8 RPL12 6136
Two-hybrid Homo sapiens
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