Gene ontology annotations for Tuba1a
Experiment description of studies that identified Tuba1a in exosomes
1
Experiment ID
907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
37309723
Organism
Mus musculus
Experiment description
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors
Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name
Proteomics
Publication year
2023
Sample
Colon cancer cells
Sample name
C26
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
2
Experiment ID
15
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63
EV Enriched markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
17486113
Organism
Mus musculus Homo sapiens
Experiment description
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors
Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name
NCB
Publication year
2007
Sample
Mast cells
Sample name
MC9 Bone marrow-derived mast cells HMC-1
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.11-1.21 g/mL
Molecules identified in the study
Protein mRNA miRNA
Methods used in the study
Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array
3
Experiment ID
39
MISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|HSC70|FLOT1
EV Enriched markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
15210972
Organism
Mus musculus
Experiment description
Cells release prions in association with exosomes.
Authors
Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name
PNAS
Publication year
2004
Sample
Mov neuroglial cells
Sample name
Mov neuroglial cell
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.14 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QSTAR] Western blotting Immunoelectron Microscopy
4
Experiment ID
33
MISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101
EV Enriched markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21136642
Organism
Mus musculus
Experiment description
Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors
Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name
PROTEOMICS_CL
Publication year
2007
Sample
Oligodendrocytes
Sample name
Oligodendrocytes Oli-neu
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.10-1.14 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF] Western blotting Immunoelectron Microscopy
5
Experiment ID
188
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Enriched markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19351151
Organism
Mus musculus
Experiment description
Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors
Lee HS, Jeong J, Lee KJ.
Journal name
J Proteome Res
Publication year
2009
Sample
Pancreatic cells
Sample name
Pancreatic beta cell (NIT-1)
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
6
Experiment ID
908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
37309723
Organism
Mus musculus
Experiment description
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors
Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name
Proteomics
Publication year
2023
Sample
T-cell lymphoma cells
Sample name
EL4
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
Protein-protein interactions for Tuba1a
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
ACTN2
88
Two-hybrid
Homo sapiens
2
BCAR1
9564
Two-hybrid
Homo sapiens
3
GNAI2
2771
Two-hybrid
Homo sapiens
4
Hsph1
15505
Two-hybrid
Mus musculus
Reconstituted Complex
Mus musculus
Affinity Capture-Western
Mus musculus
5
MAGED1
9500
Two-hybrid
Homo sapiens
6
TM4SF1
4071
Two-hybrid
Homo sapiens
7
S100A8
6279
Two-hybrid
Homo sapiens
8
RPL12
6136
Two-hybrid
Homo sapiens
View the network
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Pathways in which Tuba1a is involved