Gene description for Hsph1
Gene name heat shock 105kDa/110kDa protein 1
Gene symbol Hsph1
Other names/aliases 105kDa
AI790491
Hsp105
Hsp110
hsp-E7I
hsp110/105
Species Mus musculus
 Database cross references - Hsph1
ExoCarta ExoCarta_15505
Vesiclepedia VP_15505
Entrez Gene 15505
UniProt Q61699  
 Hsph1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hsph1
Molecular Function
    protein binding GO:0005515 IPI
    nucleotide binding GO:0000166 IEA
    ATP binding GO:0005524 IEA
    alpha-tubulin binding GO:0043014 IDA
Biological Process
    negative regulation of p38MAPK cascade GO:1903753 IGI
    negative regulation of establishment of protein localization to mitochondrion GO:1903748 IMP
    positive regulation of protein tyrosine kinase activity GO:0061098 IDA
    regulation of microtubule cytoskeleton organization GO:0070507 NAS
    chaperone mediated protein folding requiring cofactor GO:0051085 IDA
    negative regulation of apoptotic signaling pathway GO:2001234 IMP
    positive regulation of NK T cell activation GO:0051135 TAS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IGI
    positive regulation of MHC class I biosynthetic process GO:0045345 TAS
    negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide GO:1903751 IMP
    negative regulation of neuron apoptotic process GO:0043524 TAS
    response to unfolded protein GO:0006986 TAS
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    nucleoplasm GO:0005654 ISO
    cytosol GO:0005829 ISO
    microtubule GO:0005874 IDA
    cytoplasm GO:0005737 ISO
    nucleus GO:0005634 IDA
    extracellular region GO:0005576 TAS
 Experiment description of studies that identified Hsph1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hsph1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tuba1a 22142
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
View the network image/svg+xml
 Pathways in which Hsph1 is involved
PathwayEvidenceSource
Regulation of HSF1-mediated heat shock response IEA Reactome
Scavenging by Class F Receptors IEA Reactome





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