Gene description for DNAJB4
Gene name DnaJ (Hsp40) homolog, subfamily B, member 4
Gene symbol DNAJB4
Other names/aliases DNAJW
DjB4
HLJ1
Species Homo sapiens
 Database cross references - DNAJB4
ExoCarta ExoCarta_11080
Vesiclepedia VP_11080
Entrez Gene 11080
HGNC 14886
MIM 611327
UniProt Q9UDY4  
 DNAJB4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for DNAJB4
Molecular Function
    ATPase activator activity GO:0001671 IDA
    protein binding GO:0005515 IPI
    unfolded protein binding GO:0051082 IBA
    protein-folding chaperone binding GO:0051087 IBA
    protein-folding chaperone binding GO:0051087 IPI
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IBA
    response to unfolded protein GO:0006986 TAS
    response to heat GO:0009408 TAS
    chaperone cofactor-dependent protein refolding GO:0051085 IBA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    Z disc GO:0030018 IDA
 Experiment description of studies that identified DNAJB4 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DNAJB4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GSTP1 2950
Affinity Capture-MS Homo sapiens
2 ISG15 9636
Affinity Capture-MS Homo sapiens
3 ANK2 287
Proximity Label-MS Homo sapiens
4 GIGYF1  
Affinity Capture-MS Homo sapiens
5 HSPA6 3310
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
6 TPTE2  
Affinity Capture-MS Homo sapiens
7 TGS1  
Affinity Capture-MS Homo sapiens
8 HSPA1L 3305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 RANBP3 8498
Co-fractionation Homo sapiens
10 LOXL4 84171
Affinity Capture-MS Homo sapiens
11 Myh3  
Affinity Capture-MS Mus musculus
12 DNAJB6 10049
Affinity Capture-MS Homo sapiens
13 MS4A15  
Affinity Capture-MS Homo sapiens
14 WDR41  
Proximity Label-MS Homo sapiens
15 TBC1D22A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 OR6T1  
Affinity Capture-MS Homo sapiens
17 ABHD15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 RCN2 5955
Affinity Capture-MS Homo sapiens
19 CARD8  
Affinity Capture-MS Homo sapiens
20 TMEM231 79583
Affinity Capture-MS Homo sapiens
21 CCDC138  
Proximity Label-MS Homo sapiens
22 OPRM1 4988
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
23 NPM1 4869
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
24 HSPH1 10808
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 DNAJB1 3337
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 STIP1 10963
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
27 PLD2 5338
Affinity Capture-MS Homo sapiens
28 ASB4  
Affinity Capture-MS Homo sapiens
29 CRMP1 1400
Co-fractionation Homo sapiens
30 DNAJC7 7266
Affinity Capture-MS Homo sapiens
31 PELI3  
Affinity Capture-MS Homo sapiens
32 NPAS1  
Affinity Capture-MS Homo sapiens
33 SYNM 23336
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 MAS1  
Affinity Capture-MS Homo sapiens
35 HSPA8 3312
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 LCMT2 9836
Affinity Capture-MS Homo sapiens
37 BAG5 9529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 MANSC1 54682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 KLHL34  
Affinity Capture-MS Homo sapiens
40 NBR1 4077
Proximity Label-MS Homo sapiens
41 WRAP73 49856
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 SIM2  
Affinity Capture-MS Homo sapiens
43 ATP5O 539
Proximity Label-MS Homo sapiens
44 Slc8a1  
Affinity Capture-Western Mus musculus
45 NUDC 10726
Affinity Capture-MS Homo sapiens
46 WDR72  
Affinity Capture-MS Homo sapiens
47 CBX5 23468
Proximity Label-MS Homo sapiens
48 SMAD3 4088
Two-hybrid Homo sapiens
49 TTLL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 GLB1 2720
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 PYCRL 65263
Affinity Capture-MS Homo sapiens
52 Bag2  
Affinity Capture-MS Mus musculus
53 PABPN1 8106
Two-hybrid Homo sapiens
54 CCDC61  
Proximity Label-MS Homo sapiens
55 DNAJB5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 Ksr1  
Affinity Capture-MS Mus musculus
57 RAD21 5885
Affinity Capture-Western Homo sapiens
58 TTLL12 23170
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 BAG3 9531
Affinity Capture-MS Homo sapiens
Affinity Capture-Luminescence Homo sapiens
60 HSPA5 3309
Affinity Capture-MS Homo sapiens
61 TLE2  
Affinity Capture-MS Homo sapiens
62 BAG2 9532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 HERC2P9  
Affinity Capture-MS Homo sapiens
64 Ttll12 223723
Affinity Capture-MS Mus musculus
65 YWHAZ 7534
Affinity Capture-MS Homo sapiens
66 ETF1 2107
Co-fractionation Homo sapiens
67 PLD5  
Affinity Capture-MS Homo sapiens
68 GDI2 2665
Affinity Capture-MS Homo sapiens
69 MLF1  
Affinity Capture-MS Homo sapiens
70 KCNE3  
Affinity Capture-MS Homo sapiens
71 EIF4H 7458
Affinity Capture-MS Homo sapiens
72 ANGPTL4 51129
Affinity Capture-MS Homo sapiens
73 TMEM260  
Affinity Capture-MS Homo sapiens
74 PSPC1 55269
Co-fractionation Homo sapiens
75 BAG1 573
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
76 LTV1  
Proximity Label-MS Homo sapiens
77 DNAJC9 23234
Affinity Capture-MS Homo sapiens
78 TIGAR 57103
Proximity Label-MS Homo sapiens
79 HSPA1A 3303
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
80 TBL1X 6907
Two-hybrid Homo sapiens
81 HSPA4 3308
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 HYOU1 10525
Co-fractionation Homo sapiens
83 KIAA0368 23392
Co-fractionation Homo sapiens
84 ARGLU1  
Affinity Capture-MS Homo sapiens
85 DET1  
Affinity Capture-MS Homo sapiens
86 HSPA9 3313
Co-fractionation Homo sapiens
87 TSR1 55720
Proximity Label-MS Homo sapiens
88 MDM2  
Two-hybrid Homo sapiens
89 KHDC1  
Affinity Capture-MS Homo sapiens
90 PROSC 11212
Co-fractionation Homo sapiens
91 AHSA1 10598
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
92 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
93 FBXW11  
Affinity Capture-MS Homo sapiens
94 RPS25 6230
Proximity Label-MS Homo sapiens
95 GRB2 2885
Proximity Label-MS Homo sapiens
96 PGRMC1 10857
Proximity Label-MS Homo sapiens
97 L3MBTL2  
Proximity Label-MS Homo sapiens
98 RUVBL2 10856
Two-hybrid Homo sapiens
99 APOBEC3D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 KLHL26  
Affinity Capture-MS Homo sapiens
101 KCNC4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 NLRP8 126205
Affinity Capture-MS Homo sapiens
103 MLF2 8079
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 GRPEL1 80273
Co-fractionation Homo sapiens
105 SDIM1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
106 SHMT2 6472
Affinity Capture-RNA Homo sapiens
107 NRBP1 29959
Proximity Label-MS Homo sapiens
108 HSPA1B 3304
Affinity Capture-MS Homo sapiens
109 Hsph1 15505
Affinity Capture-MS Mus musculus
110 HSPA2 3306
Affinity Capture-MS Homo sapiens
111 MOV10 4343
Affinity Capture-RNA Homo sapiens
112 TBL1Y  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 C3orf38 285237
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 INF2 64423
Affinity Capture-MS Homo sapiens
115 METTL3  
Affinity Capture-MS Homo sapiens
116 POLR1D 51082
Two-hybrid Homo sapiens
117 MYC  
Affinity Capture-MS Homo sapiens
118 ST13 6767
Proximity Label-MS Homo sapiens
119 TBPL2  
Two-hybrid Homo sapiens
120 P2RX6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
121 MRGPRX4  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DNAJB4 is involved
No pathways found





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