Gene description for ANK2
Gene name ankyrin 2, neuronal
Gene symbol ANK2
Other names/aliases ANK-2
LQT4
brank-2
Species Homo sapiens
 Database cross references - ANK2
ExoCarta ExoCarta_287
Vesiclepedia VP_287
Entrez Gene 287
HGNC 493
MIM 106410
UniProt Q01484  
 ANK2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ANK2
Molecular Function
    structural constituent of cytoskeleton GO:0005200 IEA
    protein binding GO:0005515 IPI
    cytoskeletal anchor activity GO:0008093 IBA
    enzyme binding GO:0019899 IPI
    protein kinase binding GO:0019901 IPI
    spectrin binding GO:0030507 IBA
    spectrin binding GO:0030507 IPI
    protein-macromolecule adaptor activity GO:0030674 ISS
    transmembrane transporter binding GO:0044325 IBA
    transmembrane transporter binding GO:0044325 IPI
    transmembrane transporter binding GO:0044325 ISS
    ATPase binding GO:0051117 ISS
    phosphorylation-dependent protein binding GO:0140031 IPI
Biological Process
    regulation of heart rate GO:0002027 IMP
    atrial septum development GO:0003283 IMP
    intracellular calcium ion homeostasis GO:0006874 ISS
    endocytosis GO:0006897 IEA
    cytoskeleton organization GO:0007010 IEA
    protein localization GO:0008104 IGI
    positive regulation of gene expression GO:0010628 IGI
    regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion GO:0010881 IGI
    regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion GO:0010881 ISS
    regulation of cardiac muscle contraction by calcium ion signaling GO:0010882 IMP
    protein transport GO:0015031 IEA
    paranodal junction assembly GO:0030913 IEA
    regulation of protein stability GO:0031647 IC
    T-tubule organization GO:0033292 ISS
    protein localization to organelle GO:0033365 IGI
    protein localization to cell surface GO:0034394 ISS
    protein localization to M-band GO:0036309 ISS
    protein localization to T-tubule GO:0036371 ISS
    positive regulation of potassium ion transport GO:0043268 ISS
    protein stabilization GO:0050821 ISS
    regulation of release of sequestered calcium ion into cytosol GO:0051279 IGI
    response to methylmercury GO:0051597 IEA
    regulation of calcium ion transport GO:0051924 IGI
    positive regulation of calcium ion transport GO:0051928 ISS
    regulation of cardiac muscle contraction GO:0055117 IMP
    regulation of ventricular cardiac muscle cell membrane repolarization GO:0060307 IMP
    sarcoplasmic reticulum calcium ion transport GO:0070296 TAS
    protein localization to endoplasmic reticulum GO:0070972 IGI
    protein localization to plasma membrane GO:0072659 IBA
    protein localization to plasma membrane GO:0072659 IGI
    protein localization to plasma membrane GO:0072659 ISS
    regulation of cardiac muscle cell contraction GO:0086004 IGI
    ventricular cardiac muscle cell action potential GO:0086005 IMP
    atrial cardiac muscle cell action potential GO:0086014 IMP
    SA node cell action potential GO:0086015 ISS
    membrane depolarization during SA node cell action potential GO:0086046 TAS
    atrial cardiac muscle cell to AV node cell communication GO:0086066 ISS
    SA node cell to atrial cardiac muscle cell communication GO:0086070 IMP
    regulation of heart rate by cardiac conduction GO:0086091 IMP
    regulation of heart rate by cardiac conduction GO:0086091 ISS
    regulation of SA node cell action potential GO:0098907 IMP
    regulation of atrial cardiac muscle cell action potential GO:0098910 IMP
    positive regulation of potassium ion transmembrane transporter activity GO:1901018 ISS
    regulation of calcium ion transmembrane transporter activity GO:1901019 ISS
    positive regulation of calcium ion transmembrane transporter activity GO:1901021 ISS
    positive regulation of cation channel activity GO:2001259 ISS
Subcellular Localization
    mitochondrion GO:0005739 IEA
    lysosome GO:0005764 IEA
    early endosome GO:0005769 IEA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 NAS
    intercalated disc GO:0014704 ISS
    basolateral plasma membrane GO:0016323 IDA
    apical plasma membrane GO:0016324 IEA
    Z disc GO:0030018 ISS
    T-tubule GO:0030315 ISS
    M band GO:0031430 ISS
    A band GO:0031672 ISS
    sarcolemma GO:0042383 ISS
    neuron projection GO:0043005 IBA
    costamere GO:0043034 ISS
    postsynaptic membrane GO:0045211 IEA
    recycling endosome GO:0055037 IEA
 Experiment description of studies that identified ANK2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ANK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PPP1R12B 4660
Affinity Capture-MS Homo sapiens
2 KIAA0368 23392
Two-hybrid Homo sapiens
3 ACOT7 11332
Affinity Capture-MS Homo sapiens
4 GPM6A 2823
Affinity Capture-MS Homo sapiens
5 DNAAF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 DNAJB4 11080
Proximity Label-MS Homo sapiens
7 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
8 OCLN 100506658
Proximity Label-MS Homo sapiens
9 TP53 7157
Reconstituted Complex Homo sapiens
10 MARCKS 4082
Proximity Label-MS Homo sapiens
11 PRNP 5621
Affinity Capture-MS Homo sapiens
12 HIST1H4A 8359
Cross-Linking-MS (XL-MS) Homo sapiens
13 EPB42 2038
Reconstituted Complex Homo sapiens
14 CCT2 10576
Affinity Capture-MS Homo sapiens
15 CHL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 PFN1 5216
Proximity Label-MS Homo sapiens
17 BTF3 689
Affinity Capture-MS Homo sapiens
18 CTNNB1 1499
Proximity Label-MS Homo sapiens
19 CHST5  
Affinity Capture-MS Homo sapiens
20 KIF20A 10112
Affinity Capture-MS Homo sapiens
21 NDEL1 81565
Two-hybrid Homo sapiens
22 PALM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 LYN 4067
Proximity Label-MS Homo sapiens
24 MAPK8IP1 9479
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 UBAC2 337867
Affinity Capture-MS Homo sapiens
26 HIF1AN 55662
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 MYO1D 4642
Affinity Capture-MS Homo sapiens
28 TAF9 6880
Reconstituted Complex Homo sapiens
29 CRNKL1 51340
Affinity Capture-MS Homo sapiens
30 NTRK1 4914
Affinity Capture-MS Homo sapiens
31 RAPGEF5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 RYBP  
Cross-Linking-MS (XL-MS) Homo sapiens
33 DGUOK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 HIST2H3A 333932
Cross-Linking-MS (XL-MS) Homo sapiens
36 BBX 56987
Cross-Linking-MS (XL-MS) Homo sapiens
37 TNIK 23043
Two-hybrid Homo sapiens
38 SIGMAR1 10280
Affinity Capture-Western Homo sapiens
39 HAX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 SEC61G 23480
Cross-Linking-MS (XL-MS) Homo sapiens
41 DNAJB1 3337
Proximity Label-MS Homo sapiens
42 ABCE1 6059
Affinity Capture-MS Homo sapiens
43 FTSJ3 117246
Affinity Capture-MS Homo sapiens
44 FLOT1 10211
Proximity Label-MS Homo sapiens
45 FARP1 10160
Affinity Capture-MS Homo sapiens
46 ATRX 546
Cross-Linking-MS (XL-MS) Homo sapiens
47 POPDC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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