Gene description for ANK2
Gene name ankyrin 2, neuronal
Gene symbol ANK2
Other names/aliases ANK-2
LQT4
brank-2
Species Homo sapiens
 Database cross references - ANK2
ExoCarta ExoCarta_287
Vesiclepedia VP_287
Entrez Gene 287
HGNC 493
MIM 106410
UniProt Q01484  
 ANK2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ANK2
Molecular Function
    protein binding, bridging GO:0030674 ISS
    enzyme binding GO:0019899 IPI
    ATPase binding GO:0051117 ISS
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
    spectrin binding GO:0030507 IPI
    structural constituent of cytoskeleton GO:0005200 IEA
    potassium channel regulator activity GO:0015459 IEA
    ion channel binding GO:0044325 ISS
Biological Process
    protein localization to plasma membrane GO:0072659 ISS
    positive regulation of potassium ion transport GO:0043268 ISS
    regulation of cardiac muscle cell contraction GO:0086004 IGI
    regulation of cardiac muscle contraction GO:0055117 IMP
    regulation of protein stability GO:0031647 IC
    protein targeting to plasma membrane GO:0072661 IEA
    cellular protein localization GO:0034613 IGI
    regulation of cardiac muscle cell membrane potential GO:0086036 IEA
    protein localization to cell surface GO:0034394 ISS
    protein localization to M-band GO:0036309 ISS
    SA node cell action potential GO:0086015 ISS
    regulation of release of sequestered calcium ion into cytosol GO:0051279 IGI
    regulation of SA node cell action potential GO:0098907 IMP
    regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion GO:0010881 ISS
    axon guidance GO:0007411 TAS
    positive regulation of potassium ion transmembrane transporter activity GO:1901018 ISS
    positive regulation of calcium ion transmembrane transporter activity GO:1901021 ISS
    regulation of heart rate GO:0002027 IMP
    positive regulation of cation channel activity GO:2001259 ISS
    atrial cardiac muscle cell action potential GO:0086014 IMP
    membrane depolarization during SA node cell action potential GO:0086046 TAS
    protein stabilization GO:0050821 ISS
    response to methylmercury GO:0051597 IEA
    protein localization to T-tubule GO:0036371 ISS
    ventricular cardiac muscle cell action potential GO:0086005 IMP
    regulation of heart rate by cardiac conduction GO:0086091 ISS
    atrial septum development GO:0003283 IMP
    regulation of atrial cardiac muscle cell action potential GO:0098910 IMP
    sarcoplasmic reticulum calcium ion transport GO:0070296 TAS
    regulation of calcium ion transmembrane transporter activity GO:1901019 ISS
    SA node cell to atrial cardiac muscle cell communication GO:0086070 IMP
    atrial cardiac muscle cell to AV node cell communication GO:0086066 ISS
    T-tubule organization GO:0033292 ISS
    protein localization to organelle GO:0033365 IGI
    cardiac muscle contraction GO:0060048 IEA
    paranodal junction assembly GO:0030913 IEA
    protein localization to endoplasmic reticulum GO:0070972 IGI
    cellular calcium ion homeostasis GO:0006874 ISS
    regulation of calcium ion transport GO:0051924 IGI
    positive regulation of calcium ion transport GO:0051928 ISS
    regulation of ventricular cardiac muscle cell membrane repolarization GO:0060307 IMP
    positive regulation of gene expression GO:0010628 IGI
    regulation of cardiac muscle contraction by calcium ion signaling GO:0010882 IMP
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 IEA
    neuron projection GO:0043005 IEA
    cytosol GO:0005829 TAS
    M band GO:0031430 ISS
    basolateral plasma membrane GO:0016323 IDA
    apical plasma membrane GO:0016324 IEA
    intercalated disc GO:0014704 ISS
    postsynaptic membrane GO:0045211 IEA
    costamere GO:0043034 ISS
    Z disc GO:0030018 ISS
    T-tubule GO:0030315 ISS
    cytoskeleton GO:0005856 IEA
    plasma membrane GO:0005886 ISS
    integral component of plasma membrane GO:0005887 IEA
    sarcolemma GO:0042383 ISS
    membrane raft GO:0045121 IEA
    A band GO:0031672 ISS
    intracellular GO:0005622 IGI
 Experiment description of studies that identified ANK2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ANK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 L1CAM 3897
Invivo Homo sapiens
2 SIGMAR1 10280
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which ANK2 is involved
PathwayEvidenceSource
Interaction between L1 and Ankyrins TAS Reactome





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