Experiment description of studies that identified BBX in sEVs

1 Experiment ID 226 MISEV standards ✔ EM Biophysical techniques ✔ GAPDH|CD9|FLOT1 Enriched markers ✔ LMNA|H2AFX|ATP5A1|TOMM20 Negative markers ✘ Particle analysis Identified molecule mRNA Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa) PubMed ID 26027894     Organism Homo sapiens Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting" Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar" Journal name Journal of Extracellular Vesicles Publication year 2015 Sample Endothelial cells Sample name HMEC-1 Isolation/purification methods Differential ultracentrifugation Sucrose density gradient Flotation density 1.10 g/mL Molecules identified in the study miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting

2 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for BBX

Protein Interactor ExoCarta ID Identification method PubMed Species 1 GNL2 29889 Affinity Capture-MS Homo sapiens 2 SENP3 26168 Affinity Capture-MS Homo sapiens 3 RPL26L1 51121 Affinity Capture-MS Homo sapiens 4 CHIC1   Affinity Capture-MS Homo sapiens 5 ANK2 287 Cross-Linking-MS (XL-MS) Homo sapiens 6 COIL   Proximity Label-MS Homo sapiens 7 KLF9 687 Proximity Label-MS Homo sapiens 8 RPA2 6118 Affinity Capture-MS Homo sapiens 9 Nek2   Affinity Capture-MS Mus musculus 10 RPL13A 23521 Affinity Capture-MS Homo sapiens 11 HIST1H2BG 8339 Proximity Label-MS Homo sapiens 12 APC   Affinity Capture-MS Homo sapiens 13 RPL15 6138 Affinity Capture-MS Homo sapiens 14 RSBN1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 15 APP 351 Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens 16 RPL22 6146 Affinity Capture-MS Homo sapiens 17 TRIM54   Two-hybrid Homo sapiens 18 CENPA   Proximity Label-MS Homo sapiens 19 APEX1 328 Proximity Label-MS Homo sapiens 20 GPATCH4 54865 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 21 S100A4 6275 Affinity Capture-MS Homo sapiens 22 Ints4   Affinity Capture-MS Mus musculus 23 ANOS1 3730 Affinity Capture-MS Homo sapiens 24 POLR1E   Proximity Label-MS Homo sapiens 25 ZSCAN31   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 26 Cenpe   Affinity Capture-MS Mus musculus 27 ADARB1 104 Affinity Capture-MS Homo sapiens 28 MECP2 4204 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 29 H2AFY2 55506 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 30 MYCN   Affinity Capture-MS Homo sapiens 31 KLF16   Proximity Label-MS Homo sapiens 32 SHPRH   Affinity Capture-MS Homo sapiens 33 HIST1H4A 8359 Affinity Capture-MS Homo sapiens 34 MCC 4163 Affinity Capture-MS Homo sapiens 35 RPL4 6124 Affinity Capture-MS Homo sapiens 36 SRSF6 6431 Affinity Capture-MS Homo sapiens 37 NOP2 4839 Affinity Capture-MS Homo sapiens 38 CBX3 11335 Proximity Label-MS Homo sapiens 39 ANAPC15   Affinity Capture-MS Homo sapiens 40 RPL18A 6142 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 41 RPL31 6160 Proximity Label-MS Homo sapiens 42 HIST1H1E 3008 Cross-Linking-MS (XL-MS) Homo sapiens 43 RPL27A 6157 Affinity Capture-MS Homo sapiens 44 SPANXN2   Affinity Capture-MS Homo sapiens 45 MAGEB2 4113 Affinity Capture-MS Homo sapiens 46 ARID4A   Affinity Capture-MS Homo sapiens 47 CD3EAP   Proximity Label-MS Homo sapiens 48 FGFBP1 9982 Affinity Capture-MS Homo sapiens 49 CD6 923 Affinity Capture-MS Homo sapiens 50 PTEN 5728 Affinity Capture-MS Homo sapiens 51 SIN3B   Affinity Capture-MS Homo sapiens 52 HNRNPCL2 440563 Affinity Capture-MS Homo sapiens 53 CCDC85A   Affinity Capture-MS Homo sapiens 54 CDC42 998 Affinity Capture-MS Homo sapiens 55 BLK 640 Affinity Capture-MS Homo sapiens 56 ZNF512   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 57 PRR3   Affinity Capture-MS Homo sapiens 58 TRIM44   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 59 RPA3 6119 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens 60 RPS13 6207 Affinity Capture-MS Homo sapiens 61 SCGN   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 62 ZNF653   Affinity Capture-MS Homo sapiens 63 H2AFB2   Affinity Capture-MS Homo sapiens 64 LMNA 4000 Proximity Label-MS Homo sapiens 65 FHL2 2274 Affinity Capture-MS Homo sapiens 66 NEDD4L 23327 Biochemical Activity Homo sapiens 67 PPP1R15A   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 68 RPS16 6217 Affinity Capture-MS Homo sapiens 69 Cbx1   Affinity Capture-MS Mus musculus 70 SURF6   Affinity Capture-MS Homo sapiens 71 Tubg1 103733 Affinity Capture-MS Mus musculus 72 PARP1 142 Proximity Label-MS Homo sapiens 73 ILF3 3609 Affinity Capture-MS Homo sapiens 74 RPLP0 6175 Affinity Capture-MS Homo sapiens 75 FOXJ2   Affinity Capture-MS Homo sapiens 76 RPA1 6117 Affinity Capture-MS Homo sapiens 77 ELAVL1 1994 Affinity Capture-RNA Homo sapiens 78 HDAC1 3065 Affinity Capture-MS Homo sapiens 79 HSP90AA1 3320 Affinity Capture-Luminescence Homo sapiens 80 MKI67   Affinity Capture-MS Homo sapiens 81 NIFK 84365 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 82 SPAG1 6674 Affinity Capture-MS Homo sapiens 83 ZCCHC10   Affinity Capture-MS Homo sapiens 84 RPS6 6194 Proximity Label-MS Homo sapiens 85 RPL28 6158 Affinity Capture-MS Homo sapiens 86 RAB5C 5878 Affinity Capture-MS Homo sapiens 87 ZNF467   Affinity Capture-MS Homo sapiens 88 ARIH1 25820 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens View the network

Pathways in which BBX is involved

No pathways found