Gene description for RPL22
Gene name ribosomal protein L22
Gene symbol RPL22
Other names/aliases EAP
HBP15
HBP15/L22
L22
Species Homo sapiens
 Database cross references - RPL22
ExoCarta ExoCarta_6146
Entrez Gene 6146
HGNC 10315
MIM 180474
UniProt P35268  
 RPL22 identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
Thymus 23844026    
 Gene ontology annotations for RPL22
Molecular Function
    RNA binding GO:0003723 TAS
    structural constituent of ribosome GO:0003735 NAS
    protein binding GO:0005515 IPI
    heparin binding GO:0008201 IEA
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    translational elongation GO:0006414 TAS
    translational termination GO:0006415 TAS
    gene expression GO:0010467 TAS
    viral transcription GO:0019083 TAS
    cellular protein metabolic process GO:0044267 TAS
    alpha-beta T cell differentiation GO:0046632 IEA
    viral life cycle GO:0019058 TAS
    translation GO:0006412 TAS
    viral process GO:0016032 TAS
    translational initiation GO:0006413 TAS
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 TAS
    SRP-dependent cotranslational protein targeting to membrane GO:0006614 TAS
Subcellular Localization
    nucleus GO:0005634 IDA
    extracellular exosome GO:0070062 IDA
    cytosolic large ribosomal subunit GO:0022625 IDA
    focal adhesion GO:0005925 IDA
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
    ribonucleoprotein complex GO:0030529 IDA
 Experiment description of studies that identified RPL22 in exosomes
1
Experiment ID 79
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RPL22
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NSFL1C 55968
Two-hybrid Homo sapiens
2 CSE1L 1434
Two-hybrid Homo sapiens
View the network image/svg+xml



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