Gene description for CWC22
Gene name CWC22 spliceosome-associated protein
Gene symbol CWC22
Other names/aliases EIF4GL
NCM
fSAPb
Species Homo sapiens
 Database cross references - CWC22
ExoCarta ExoCarta_57703
Vesiclepedia VP_57703
Entrez Gene 57703
HGNC 29322
MIM 615186
UniProt Q9HCG8  
 CWC22 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for CWC22
Molecular Function
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 IDA
    protein binding GO:0005515 IPI
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 IC
    mRNA splicing, via spliceosome GO:0000398 IDA
    regulation of mRNA splicing, via spliceosome GO:0048024 IC
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    spliceosomal complex GO:0005681 IDA
    cytosol GO:0005829 IDA
    nuclear speck GO:0016607 IDA
    U2-type precatalytic spliceosome GO:0071005 IDA
    U2-type catalytic step 1 spliceosome GO:0071006 IDA
    U2-type catalytic step 2 spliceosome GO:0071007 IDA
    catalytic step 2 spliceosome GO:0071013 IBA
    catalytic step 2 spliceosome GO:0071013 IDA
 Experiment description of studies that identified CWC22 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CWC22
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 ATG16L1 55054
Affinity Capture-MS Homo sapiens
3 CAMKV 79012
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 GRK5 2869
Affinity Capture-MS Homo sapiens
5 ERMAP 114625
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 HNRNPC 3183
Affinity Capture-MS Homo sapiens
7 Utp15  
Two-hybrid Mus musculus
8 SNX27 81609
Affinity Capture-MS Homo sapiens
9 CD2BP2 10421
Affinity Capture-MS Homo sapiens
10 MYCN  
Affinity Capture-MS Homo sapiens
11 EAPP  
Affinity Capture-MS Homo sapiens
12 USP7 7874
Affinity Capture-MS Homo sapiens
13 EPB41L4B 54566
Affinity Capture-MS Homo sapiens
14 VTI1B 10490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 C11orf57  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 FRG1 2483
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
17 SIGLECL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 KIF14 9928
Affinity Capture-MS Homo sapiens
19 BTNL9  
Affinity Capture-MS Homo sapiens
20 PES1 23481
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 C9orf78 51759
Affinity Capture-MS Homo sapiens
22 UBE2H 7328
Affinity Capture-MS Homo sapiens
23 MDK 4192
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 CTBP2 1488
Affinity Capture-MS Homo sapiens
25 S100A6 6277
Affinity Capture-MS Homo sapiens
26 EPB41L3 23136
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 PDAP1 11333
Two-hybrid Homo sapiens
28 ZNHIT2 741
Affinity Capture-MS Homo sapiens
29 BTC  
Affinity Capture-MS Homo sapiens
30 EPB41L5 57669
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 PLCD3 113026
Affinity Capture-MS Homo sapiens
32 PDHA1 5160
Affinity Capture-MS Homo sapiens
33 DOK4 55715
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 CHST5  
Affinity Capture-MS Homo sapiens
35 PRPF19 27339
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
36 RAD18  
Affinity Capture-MS Homo sapiens
37 RPA3 6119
Proximity Label-MS Homo sapiens
38 MECP2 4204
Affinity Capture-MS Homo sapiens
39 MAP10  
Affinity Capture-MS Homo sapiens
40 DDRGK1 65992
Affinity Capture-MS Homo sapiens
41 FAM32A  
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
42 CHD4 1108
Affinity Capture-MS Homo sapiens
43 EIF4A3 9775
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
44 EPB41L2 2037
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 SNRPA 6626
Affinity Capture-MS Homo sapiens
46 PTGER3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
48 SEC62 7095
Affinity Capture-MS Homo sapiens
49 EGFR 1956
Negative Genetic Homo sapiens
50 DDX23 9416
Proximity Label-MS Homo sapiens
51 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 EDA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 CTDSP2 10106
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 SULF2 55959
Affinity Capture-MS Homo sapiens
55 FGFBP1 9982
Affinity Capture-MS Homo sapiens
56 FAM9A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 DENND2D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 PARK2  
Affinity Capture-MS Homo sapiens
59 NTRK1 4914
Affinity Capture-MS Homo sapiens
60 FGF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 CREB3L3  
Affinity Capture-MS Homo sapiens
62 NFKBIL1  
Affinity Capture-MS Homo sapiens
63 TSSC4  
Affinity Capture-MS Homo sapiens
64 RPL22 6146
Affinity Capture-MS Homo sapiens
65 SYT1 6857
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 EPB41L1 2036
Affinity Capture-MS Homo sapiens
67 KRAS 3845
Synthetic Growth Defect Homo sapiens
68 KLHL2 11275
Two-hybrid Homo sapiens
69 YWHAG 7532
Affinity Capture-MS Homo sapiens
70 Bud13  
Affinity Capture-MS Mus musculus
71 SYT2  
Affinity Capture-MS Homo sapiens
72 SRSF7 6432
Affinity Capture-MS Homo sapiens
73 PRC1 9055
Affinity Capture-MS Homo sapiens
74 CPSF6 11052
Affinity Capture-MS Homo sapiens
75 MKI67  
Affinity Capture-MS Homo sapiens
76 GPR183  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 EXOSC8  
Two-hybrid Homo sapiens
78 CT45A5  
Affinity Capture-MS Homo sapiens
79 EED  
Affinity Capture-MS Homo sapiens
80 FMR1NB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here