Gene description for SYT1
Gene name synaptotagmin I
Gene symbol SYT1
Other names/aliases P65
SVP65
SYT
Species Homo sapiens
 Database cross references - SYT1
ExoCarta ExoCarta_6857
Vesiclepedia VP_6857
Entrez Gene 6857
HGNC 11509
MIM 185605
UniProt P21579  
 SYT1 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for SYT1
Molecular Function
    SNARE binding GO:0000149 IBA
    SNARE binding GO:0000149 ISS
    phosphatidylserine binding GO:0001786 IEA
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
    calcium-dependent phospholipid binding GO:0005544 IBA
    calcium-dependent phospholipid binding GO:0005544 ISS
    calcium-dependent phospholipid binding GO:0005544 TAS
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IEA
    lipid binding GO:0008289 EXP
    lipid binding GO:0008289 IDA
    syntaxin-1 binding GO:0017075 ISS
    clathrin binding GO:0030276 IEA
    syntaxin-3 binding GO:0030348 IEA
    identical protein binding GO:0042802 IEA
    protein heterodimerization activity GO:0046982 IEA
    calcium-dependent protein binding GO:0048306 IEA
    low-density lipoprotein particle receptor binding GO:0050750 IDA
    calcium ion sensor activity GO:0061891 IBA
    molecular condensate scaffold activity GO:0140693 IEA
Biological Process
    detection of calcium ion GO:0005513 TAS
    vesicle fusion GO:0006906 IEA
    chemical synaptic transmission GO:0007268 TAS
    neurotransmitter secretion GO:0007269 TAS
    vesicle organization GO:0016050 IDA
    vesicle-mediated transport GO:0016192 IBA
    regulation of exocytosis GO:0017157 TAS
    regulation of calcium ion-dependent exocytosis GO:0017158 IBA
    cell differentiation GO:0030154 IEA
    positive regulation of dopamine secretion GO:0033603 IEA
    vesicle docking GO:0048278 IEA
    positive regulation of synaptic transmission GO:0050806 ISS
    protein heterooligomerization GO:0051291 IEA
    regulation of synaptic transmission, glutamatergic GO:0051966 ISS
    spontaneous neurotransmitter secretion GO:0061669 IEA
    cellular response to calcium ion GO:0071277 ISS
    synchronous neurotransmitter secretion GO:0071911 IEA
    fast, calcium ion-dependent exocytosis of neurotransmitter GO:0098746 ISS
    calcium-dependent activation of synaptic vesicle fusion GO:0099502 IBA
    membraneless organelle assembly GO:0140694 IEA
    positive regulation of calcium ion-dependent exocytosis of neurotransmitter GO:1903235 ISS
    regulation of regulated secretory pathway GO:1903305 ISS
    positive regulation of dendrite extension GO:1903861 IDA
    regulation of synaptic vesicle exocytosis GO:2000300 IBA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    Golgi apparatus GO:0005794 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    synaptic vesicle GO:0008021 TAS
    postsynaptic density GO:0014069 IEA
    axon GO:0030424 IBA
    clathrin-coated endocytic vesicle membrane GO:0030669 TAS
    synaptic vesicle membrane GO:0030672 IBA
    synaptic vesicle membrane GO:0030672 TAS
    dense core granule GO:0031045 IBA
    chromaffin granule membrane GO:0042584 IEA
    presynaptic membrane GO:0042734 IEA
    neuron projection GO:0043005 ISS
    neuron projection terminus GO:0044306 IEA
    postsynaptic membrane GO:0045211 IEA
    presynaptic active zone GO:0048786 IEA
    excitatory synapse GO:0060076 IEA
    clathrin-sculpted acetylcholine transport vesicle membrane GO:0060201 TAS
    clathrin-sculpted glutamate transport vesicle membrane GO:0060203 TAS
    clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane GO:0061202 TAS
    clathrin-sculpted monoamine transport vesicle membrane GO:0070083 TAS
    exocytic vesicle GO:0070382 IBA
    hippocampal mossy fiber to CA3 synapse GO:0098686 IEA
    glutamatergic synapse GO:0098978 IEA
    presynaptic cytosol GO:0099523 IEA
    postsynaptic cytosol GO:0099524 IEA
 Experiment description of studies that identified SYT1 in exosomes
1
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for SYT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SV2C  
Affinity Capture-Western Homo sapiens
2 RAB33A 9363
Affinity Capture-Western Homo sapiens
3 CDCA8 55143
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 PGK2 5232
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 STON2 85439
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
6 DNAAF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 SYNCRIP 10492
Reconstituted Complex Homo sapiens
8 NEDD4 4734
Reconstituted Complex Homo sapiens
9 RRP7A 27341
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 RBM14 10432
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
11 SIN3A  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
12 PRNP 5621
Affinity Capture-MS Homo sapiens
13 CHGA  
Affinity Capture-Western Homo sapiens
14 GPM6A 2823
Affinity Capture-MS Homo sapiens
15 TSHR 7253
Two-hybrid Homo sapiens
16 SYT5 6861
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 EIF2B2 8892
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 IKBKG 8517
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
19 C6orf15  
Affinity Capture-Western Homo sapiens
20 SNAP25 6616
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
21 VAMP2 6844
Affinity Capture-Western Homo sapiens
22 CREBBP  
Two-hybrid Homo sapiens
23 EIF2B3 8891
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 STX4 6810
Reconstituted Complex Homo sapiens
25 S100A13 6284
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
26 CALM1 801
Reconstituted Complex Homo sapiens
27 SDC2 6383
Affinity Capture-MS Homo sapiens
28 RBM18  
Affinity Capture-MS Homo sapiens
29 ATG16L1 55054
Affinity Capture-Western Homo sapiens
30 KCNE3  
Affinity Capture-MS Homo sapiens
31 SV2B  
Affinity Capture-Western Homo sapiens
32 STX1A 6804
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
33 STX3 6809
Reconstituted Complex Homo sapiens
34 NTRK1 4914
Affinity Capture-MS Homo sapiens
35 Caskin1  
Two-hybrid Rattus norvegicus
36 LRP2 4036
Affinity Capture-MS Homo sapiens
37 DGUOK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 BLMH 642
Affinity Capture-MS Homo sapiens
39 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 VLDLR 7436
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 CACNA1A  
Reconstituted Complex Homo sapiens
42 ARMC1 55156
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 SMAD2 4087
Reconstituted Complex Homo sapiens
44 TNFRSF10C  
Affinity Capture-MS Homo sapiens
45 MCTS1 28985
Affinity Capture-MS Homo sapiens
46 GOLM1 51280
Two-hybrid Homo sapiens
47 CWC22 57703
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 HAX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 CD63 967
Affinity Capture-MS Homo sapiens
50 SYT2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 FGF1  
Reconstituted Complex Homo sapiens
52 RAB27A 5873
Affinity Capture-Western Homo sapiens
53 EIF2B4 8890
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 EIF2B5 8893
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 STX2 2054
Reconstituted Complex Homo sapiens
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