Gene description for SYT1
Gene name synaptotagmin I
Gene symbol SYT1
Other names/aliases P65
SVP65
SYT
Species Homo sapiens
 Database cross references - SYT1
ExoCarta ExoCarta_6857
Vesiclepedia VP_6857
Entrez Gene 6857
HGNC 11509
MIM 185605
UniProt P21579  
 SYT1 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for SYT1
Molecular Function
    identical protein binding GO:0042802 IEA
    protein C-terminus binding GO:0008022 IEA
    protein binding GO:0005515 IPI
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IBA
    low-density lipoprotein particle receptor binding GO:0050750 IDA
    1-phosphatidylinositol binding GO:0005545 TAS
    clathrin binding GO:0030276 IBA
    calcium-dependent phospholipid binding GO:0005544 TAS
    calcium ion binding GO:0005509 IBA
    calcium-dependent protein binding GO:0048306 IEA
    phosphatidylserine binding GO:0001786 IEA
    syntaxin-1 binding GO:0017075 TAS
    SNARE binding GO:0000149 ISS
    calmodulin binding GO:0005516 IEA
Biological Process
    synaptic transmission GO:0007268 TAS
    vesicle docking GO:0048278 IEA
    positive regulation of calcium ion-dependent exocytosis GO:0045956 IEA
    regulation of calcium ion-dependent exocytosis GO:0017158 IBA
    glutamate secretion GO:0014047 TAS
    regulation of regulated secretory pathway GO:1903305 ISS
    regulation of synaptic transmission, glutamatergic GO:0051966 ISS
    calcium ion-dependent exocytosis of neurotransmitter GO:0048791 IBA
    synaptic vesicle exocytosis GO:0016079 IBA
    fast, calcium ion-dependent exocytosis of neurotransmitter GO:0098746 ISS
    detection of calcium ion GO:0005513 TAS
    positive regulation of dendrite extension GO:1903861 IDA
    positive regulation of vesicle fusion GO:0031340 IEA
    protein homooligomerization GO:0051260 TAS
    neurotransmitter secretion GO:0007269 TAS
    regulation of exocytosis GO:0017157 TAS
    positive regulation of synaptic transmission GO:0050806 ISS
    synaptic vesicle endocytosis GO:0048488 IBA
Subcellular Localization
    chromaffin granule membrane GO:0042584 IEA
    dense core granule GO:0031045 IEA
    clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane GO:0061202 TAS
    neuron projection GO:0043005 ISS
    presynaptic membrane GO:0042734 IEA
    excitatory synapse GO:0060076 IEA
    synaptic vesicle membrane GO:0030672 TAS
    clathrin-sculpted glutamate transport vesicle membrane GO:0060203 TAS
    clathrin-sculpted monoamine transport vesicle membrane GO:0070083 TAS
    SNARE complex GO:0031201 IEA
    cell junction GO:0030054 IEA
    plasma membrane GO:0005886 TAS
    integral component of membrane GO:0016021 IEA
    synaptic vesicle GO:0008021 TAS
    clathrin-sculpted acetylcholine transport vesicle membrane GO:0060201 TAS
 Experiment description of studies that identified SYT1 in exosomes
1
Experiment ID 126
ISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 488
ISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101|CD63|CD81
EV Enriched markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for SYT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SV2C  
Affinity Capture-Western Homo sapiens
2 STON2 85439
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
3 GNB2L1 10399
Invitro Homo sapiens
4 GOLM1 51280
Two-hybrid Homo sapiens
5 FGF1  
Invitro Homo sapiens
6 C6orf15  
Affinity Capture-Western Homo sapiens
7 CACNA1A  
Invitro Homo sapiens
8 SV2B  
Affinity Capture-Western Homo sapiens
9 STX1A 6804
Reconstituted Complex Homo sapiens
10 SNAP25 6616
Reconstituted Complex Homo sapiens
11 CALM1 801
Reconstituted Complex Homo sapiens
12 SYT4  
Invitro Homo sapiens
13 SYNCRIP 10492
Reconstituted Complex Homo sapiens
14 S100A13 6284
Co-purification Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml



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