Gene description for RAB33A
Gene name RAB33A, member RAS oncogene family
Gene symbol RAB33A
Other names/aliases RabS10
Species Homo sapiens
 Database cross references - RAB33A
ExoCarta ExoCarta_9363
Vesiclepedia VP_9363
Entrez Gene 9363
HGNC 9773
MIM 300333
UniProt Q14088  
 RAB33A identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for RAB33A
Molecular Function
    GTPase activity GO:0003924 IBA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
Biological Process
    autophagosome assembly GO:0000045 IBA
    antigen processing and presentation GO:0019882 IMP
    Rab protein signal transduction GO:0032482 IEA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    endosome GO:0005768 IBA
    Golgi apparatus GO:0005794 IBA
    plasma membrane GO:0005886 IEA
 Experiment description of studies that identified RAB33A in exosomes
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RAB33A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SIAH1  
Two-hybrid Homo sapiens
2 ATG16L1 55054
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
3 CHML  
Affinity Capture-MS Homo sapiens
4 RABGGTA 5875
Affinity Capture-MS Homo sapiens
5 ALDH4A1 8659
Affinity Capture-MS Homo sapiens
6 ATG5 9474
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
7 TMEM39B  
Affinity Capture-MS Homo sapiens
8 RTN3 10313
Two-hybrid Homo sapiens
9 XPO5 57510
Affinity Capture-MS Homo sapiens
10 DDX19B 11269
Affinity Capture-MS Homo sapiens
11 KRT34  
Two-hybrid Homo sapiens
12 ATG12  
Affinity Capture-MS Homo sapiens
13 TUBB8 347688
Affinity Capture-MS Homo sapiens
14 ARL8A 127829
Affinity Capture-MS Homo sapiens
15 GRPEL1 80273
Affinity Capture-MS Homo sapiens
16 ARL6IP1 23204
Two-hybrid Homo sapiens
17 LRP2BP  
Two-hybrid Homo sapiens
18 ERGIC1 57222
Affinity Capture-MS Homo sapiens
19 RTN4 57142
Two-hybrid Homo sapiens
20 RAB19 401409
Affinity Capture-MS Homo sapiens
21 GDI2 2665
Affinity Capture-MS Homo sapiens
22 Hoxa1  
Two-hybrid Mus musculus
PCA Mus musculus
Affinity Capture-Western Mus musculus
23 TUFM 7284
Affinity Capture-MS Homo sapiens
24 RUFY2  
Two-hybrid Homo sapiens
25 RABAC1 10567
Two-hybrid Homo sapiens
26 PSMF1 9491
Two-hybrid Homo sapiens
27 RAB33B 83452
Affinity Capture-MS Homo sapiens
28 GDI1 2664
Affinity Capture-MS Homo sapiens
29 CCDC136  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
30 CHM 1121
Affinity Capture-MS Homo sapiens
31 SYT1 6857
Affinity Capture-Western Homo sapiens
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