Gene description for RTN3
Gene name reticulon 3
Gene symbol RTN3
Other names/aliases ASYIP
HAP
NSPL2
NSPLII
RTN3-A1
Species Homo sapiens
 Database cross references - RTN3
ExoCarta ExoCarta_10313
Entrez Gene 10313
HGNC 10469
MIM 604249
UniProt O95197  
 RTN3 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Thymus 23844026    
 Gene ontology annotations for RTN3
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    apoptotic process GO:0006915 IEA
    vesicle-mediated transport GO:0016192 IEA
    endoplasmic reticulum tubular network organization GO:0071786 IMP
    viral process GO:0016032 IEA
Subcellular Localization
    extracellular space GO:0005615 TAS
    integral component of membrane GO:0016021 IEA
    endoplasmic reticulum GO:0005783 IDA
    extracellular exosome GO:0070062 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    endomembrane system GO:0012505 IBA
    Golgi membrane GO:0000139 IEA
 Experiment description of studies that identified RTN3 in exosomes
1
Experiment ID 234
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RTN3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAB33A 9363
Two-hybrid Homo sapiens
2 FAM160A2 84067
Two-hybrid Homo sapiens
3 PLEKHF2 79666
Two-hybrid Homo sapiens
4 SPOP  
Two-hybrid Homo sapiens
5 FXR2 9513
Two-hybrid Homo sapiens
6 COL4A3BP  
Two-hybrid Homo sapiens
7 UGCG 7357
Two-hybrid Homo sapiens
8 PTPN9 5780
Two-hybrid Homo sapiens
9 RTN3 10313
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 RTN4 57142
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which RTN3 is involved
No pathways found





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