Gene description for SNAP25
Gene name synaptosomal-associated protein, 25kDa
Gene symbol SNAP25
Other names/aliases CMS18
RIC-4
RIC4
SEC9
SNAP
SNAP-25
bA416N4.2
dJ1068F16.2
Species Homo sapiens
 Database cross references - SNAP25
ExoCarta ExoCarta_6616
Vesiclepedia VP_6616
Entrez Gene 6616
HGNC 11132
MIM 600322
UniProt P60880  
 SNAP25 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for SNAP25
Molecular Function
    syntaxin-1 binding GO:0017075 IPI
    calcium-dependent protein binding GO:0048306 ISS
    protein binding GO:0005515 IPI
    SNAP receptor activity GO:0005484 IBA
Biological Process
    regulation of neuron projection development GO:0010975 IEA
    small molecule metabolic process GO:0044281 TAS
    regulation of insulin secretion GO:0050796 TAS
    synaptic vesicle exocytosis GO:0016079 TAS
    synaptic vesicle priming GO:0016082 IBA
    synaptic transmission GO:0007268 TAS
    energy reserve metabolic process GO:0006112 TAS
    long-term synaptic potentiation GO:0060291 IEA
    neurotransmitter uptake GO:0001504 NAS
    glutamate secretion GO:0014047 TAS
    synaptic vesicle docking GO:0016081 NAS
    neurotransmitter secretion GO:0007269 TAS
    synaptic vesicle fusion to presynaptic membrane GO:0031629 IBA
Subcellular Localization
    SNARE complex GO:0031201 TAS
    perinuclear region of cytoplasm GO:0048471 IEA
    cell junction GO:0030054 IEA
    cytosol GO:0005829 TAS
    presynaptic membrane GO:0042734 TAS
    growth cone GO:0030426 ISS
    vesicle GO:0031982 IDA
    membrane GO:0016020 ISS
    neuron projection GO:0043005 ISS
    synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex GO:0070032 IBA
    voltage-gated potassium channel complex GO:0008076 ISS
    BLOC-1 complex GO:0031083 IDA
    plasma membrane GO:0005886 TAS
    cytoplasm GO:0005737 ISS
    trans-Golgi network GO:0005802 IEA
 Experiment description of studies that identified SNAP25 in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101|CD63|CD81
EV Enriched markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SNAP25
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VAMP2 6844
Invitro Homo sapiens
2 SYT9 143425
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
3 ITSN1 6453
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
4 STX12 23673
Invivo Homo sapiens
5 VAMP8 8673
Invitro Homo sapiens
6 GOSR1 9527
Reconstituted Complex Homo sapiens
7 STXBP6 29091
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
8 STXBP1 6812
Affinity Capture-MS Homo sapiens
9 TRIM9  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
10 SNAPIN 23557
Invitro Homo sapiens
11 STX4 6810
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
12 STX11 8676
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
13 VAMP7 6845
Reconstituted Complex Homo sapiens
14 SYT3  
Reconstituted Complex Homo sapiens
15 STX1A 6804
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 STX3 6809
Two-hybrid Homo sapiens
17 KCNB1  
Reconstituted Complex Homo sapiens
18 KIF5B 3799
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
19 HGS 9146
Invivo Homo sapiens
20 SNIP  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
21 STX5 6811
Reconstituted Complex Homo sapiens
22 PRRT2  
Two-hybrid Homo sapiens
23 SYT1 6857
Reconstituted Complex Homo sapiens
24 STX2 2054
Two-hybrid Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here