Experiment description of studies that identified GPM6A in exosomes

1 Experiment ID 363 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 364 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 365 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 254 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1|CD81 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name MNT-1 Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 255 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name G1 Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

6 Experiment ID 256 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name 501mel Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

7 Experiment ID 257 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name Daju Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

8 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

9 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

10 Experiment ID 63 MISEV standards ✘ Biophysical techniques ✔ AQP2 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19056867     Organism Homo sapiens Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes. Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA" Journal name JASN Publication year 2009 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

Protein-protein interactions for GPM6A

Protein Interactor ExoCarta ID Identification method PubMed Species 1 RNF149 284996 Affinity Capture-MS Homo sapiens 2 CAMKV 79012 Affinity Capture-MS Homo sapiens 3 YKT6 10652 Affinity Capture-MS Homo sapiens 4 DPYSL2 1808 Affinity Capture-MS Homo sapiens 5 TRIM3 10612 Affinity Capture-MS Homo sapiens 6 ANK2 287 Affinity Capture-MS Homo sapiens 7 CACNA2D1 781 Affinity Capture-MS Homo sapiens 8 DERL2 51009 Affinity Capture-MS Homo sapiens 9 C1orf27 54953 Affinity Capture-MS Homo sapiens 10 VSNL1 7447 Affinity Capture-MS Homo sapiens 11 PHYHIPL   Affinity Capture-MS Homo sapiens 12 NCALD 83988 Affinity Capture-MS Homo sapiens 13 ATP6V1C1 528 Affinity Capture-MS Homo sapiens 14 SRCIN1   Affinity Capture-MS Homo sapiens 15 SLC8A2   Affinity Capture-MS Homo sapiens 16 SEPT5 5413 Affinity Capture-MS Homo sapiens 17 GNAQ 2776 Affinity Capture-MS Homo sapiens 18 UQCR10 29796 Affinity Capture-MS Homo sapiens 19 GPR114   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 20 MGAT5 4249 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 21 ELAVL3   Affinity Capture-MS Homo sapiens 22 GP1BB 2812 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 23 CORO1A 11151 Affinity Capture-MS Homo sapiens 24 SYNGAP1   Affinity Capture-MS Homo sapiens 25 P2RY1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 26 GNAO1 2775 Affinity Capture-MS Homo sapiens 27 NR2F6   Affinity Capture-MS Homo sapiens 28 NUFIP1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 29 CADPS   Affinity Capture-MS Homo sapiens 30 YIPF3 25844 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 31 H2AFY2 55506 Affinity Capture-MS Homo sapiens 32 CAMK2B 816 Affinity Capture-MS Homo sapiens 33 ERMP1 79956 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 34 L1CAM 3897 Affinity Capture-MS Homo sapiens 35 GNAZ 2781 Affinity Capture-MS Homo sapiens 36 DNM1 1759 Affinity Capture-MS Homo sapiens 37 PPP3R1 5534 Affinity Capture-MS Homo sapiens 38 HPCAL4   Affinity Capture-MS Homo sapiens 39 SYN3   Affinity Capture-MS Homo sapiens 40 PRNP 5621 Affinity Capture-MS Homo sapiens 41 MAP2 4133 Affinity Capture-MS Homo sapiens 42 ABAT   Affinity Capture-MS Homo sapiens 43 PXMP2   Affinity Capture-MS Homo sapiens 44 CAMK2A 815 Affinity Capture-MS Homo sapiens 45 VKORC1L1 154807 Affinity Capture-MS Homo sapiens 46 NCAM1 4684 Affinity Capture-MS Homo sapiens 47 DPYSL4 10570 Affinity Capture-MS Homo sapiens 48 CCDC126 90693 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 49 IKBIP 121457 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 50 HPCA 3208 Affinity Capture-MS Homo sapiens 51 RAB14 51552 Affinity Capture-MS Homo sapiens 52 HMGCS1 3157 Affinity Capture-MS Homo sapiens 53 SNAP91 9892 Affinity Capture-MS Homo sapiens 54 TSPAN3 10099 Affinity Capture-MS Homo sapiens 55 PAM16   Two-hybrid Homo sapiens 56 COMTD1 118881 Affinity Capture-MS Homo sapiens 57 MAPT   Affinity Capture-MS Homo sapiens 58 SYNJ1 8867 Affinity Capture-MS Homo sapiens 59 SCAMP2 10066 Affinity Capture-MS Homo sapiens 60 CNTN1 1272 Affinity Capture-MS Homo sapiens 61 SEPT3   Affinity Capture-MS Homo sapiens 62 ARMC1 55156 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 63 NAPG 8774 Affinity Capture-MS Homo sapiens 64 ATP4A 495 Affinity Capture-MS Homo sapiens 65 PLXNA2 5362 Affinity Capture-MS Homo sapiens 66 CRMP1 1400 Affinity Capture-MS Homo sapiens 67 FAM49B 51571 Affinity Capture-MS Homo sapiens 68 H2AFY 9555 Affinity Capture-MS Homo sapiens 69 DNM1L 10059 Affinity Capture-MS Homo sapiens 70 BIN1 274 Affinity Capture-MS Homo sapiens 71 RMDN3 55177 Affinity Capture-MS Homo sapiens 72 ATP2B3 492 Affinity Capture-MS Homo sapiens 73 HTR3A   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 74 FAM189B   Affinity Capture-MS Homo sapiens 75 HIST1H2BL 8340 Affinity Capture-MS Homo sapiens 76 DNAAF2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 77 EPB41L1 2036 Affinity Capture-MS Homo sapiens 78 SYN2 6854 Affinity Capture-MS Homo sapiens 79 DCLK1 9201 Affinity Capture-MS Homo sapiens 80 PCDH1 5097 Affinity Capture-MS Homo sapiens 81 ATP2B2 491 Affinity Capture-MS Homo sapiens 82 DUOXA2   Affinity Capture-MS Homo sapiens 83 HPCAL1 3241 Affinity Capture-MS Homo sapiens 84 STXBP1 6812 Affinity Capture-MS Homo sapiens 85 PPID 5481 Affinity Capture-MS Homo sapiens 86 CALB2   Affinity Capture-MS Homo sapiens 87 FITM2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 88 KIF2A 3796 Affinity Capture-MS Homo sapiens 89 RAB6B 51560 Affinity Capture-MS Homo sapiens 90 AMPH 273 Affinity Capture-MS Homo sapiens 91 SYP   Affinity Capture-MS Homo sapiens 92 SLC35B2 347734 Affinity Capture-MS Homo sapiens 93 MAPRE3   Affinity Capture-MS Homo sapiens 94 STX1B 112755 Affinity Capture-MS Homo sapiens 95 NDRG4 65009 Affinity Capture-MS Homo sapiens 96 RFT1 91869 Affinity Capture-MS Homo sapiens 97 ZDHHC18 84243 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 98 EEF1A2 1917 Affinity Capture-MS Homo sapiens 99 NTM 50863 Affinity Capture-MS Homo sapiens 100 DCX   Affinity Capture-MS Homo sapiens 101 HAX1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 102 RAB3A 5864 Affinity Capture-MS Homo sapiens 103 DPYSL3 1809 Affinity Capture-MS Homo sapiens 104 WDR47   Affinity Capture-MS Homo sapiens 105 RAB3C 115827 Affinity Capture-MS Homo sapiens 106 CDH13 1012 Affinity Capture-MS Homo sapiens 107 DPP6   Affinity Capture-MS Homo sapiens 108 SNCA 6622 Affinity Capture-MS Homo sapiens 109 EDIL3 10085 Affinity Capture-MS Homo sapiens 110 VNN2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 111 DPYSL5   Affinity Capture-MS Homo sapiens 112 FAM49A 81553 Affinity Capture-MS Homo sapiens 113 RABIF   Affinity Capture-MS Homo sapiens 114 FKRP 79147 Affinity Capture-MS Homo sapiens 115 TSPAN17 26262 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 116 MAPRE2 10982 Affinity Capture-MS Homo sapiens 117 Htt   Affinity Capture-MS Mus musculus 118 SNAP25 6616 Affinity Capture-MS Homo sapiens 119 AGPAT3 56894 Affinity Capture-MS Homo sapiens 120 PRKAR2B 5577 Affinity Capture-MS Homo sapiens 121 OGDHL   Affinity Capture-MS Homo sapiens 122 NAPB 63908 Affinity Capture-MS Homo sapiens 123 ART4   Affinity Capture-MS Homo sapiens 124 TNFRSF10B 8795 Affinity Capture-MS Homo sapiens 125 EDA   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 126 PLXNA4 91584 Affinity Capture-MS Homo sapiens 127 VPS26B 112936 Affinity Capture-MS Homo sapiens 128 ZDHHC17 23390 Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 129 DNM3 26052 Affinity Capture-MS Homo sapiens 130 DGUOK   Affinity Capture-MS Homo sapiens 131 AP3B2 8120 Affinity Capture-MS Homo sapiens 132 RAC3 5881 Affinity Capture-MS Homo sapiens 133 TMED7 51014 Affinity Capture-MS Homo sapiens 134 MAP1A 4130 Affinity Capture-MS Homo sapiens 135 SYN1   Affinity Capture-MS Homo sapiens 136 STX1A 6804 Affinity Capture-MS Homo sapiens 137 SLC12A5 57468 Affinity Capture-MS Homo sapiens 138 GRIA2   Affinity Capture-MS Homo sapiens 139 SYT1 6857 Affinity Capture-MS Homo sapiens 140 CPE 1363 Affinity Capture-MS Homo sapiens View the network

Pathways in which GPM6A is involved

No pathways found