Gene description for GNAZ
Gene name guanine nucleotide binding protein (G protein), alpha z polypeptide
Gene symbol GNAZ
Other names/aliases -
Species Homo sapiens
 Database cross references - GNAZ
ExoCarta ExoCarta_2781
Entrez Gene 2781
HGNC 4395
MIM 139160
UniProt P19086  
 GNAZ identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for GNAZ
Molecular Function
    GTP binding GO:0005525 IEA
    GTPase activity GO:0003924 IEA
    metal ion binding GO:0046872 IEA
    receptor signaling protein activity GO:0005057 TAS
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    G-protein coupled serotonin receptor binding GO:0031821 IBA
Biological Process
    G-protein coupled receptor signaling pathway GO:0007186 TAS
    intracellular signal transduction GO:0035556 TAS
    adenylate cyclase-modulating G-protein coupled receptor signaling pathway GO:0007188 IBA
    adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway GO:0007193 IEA
    metabolic process GO:0008152 TAS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    nuclear envelope GO:0005635 TAS
    cytosol GO:0005829 IEA
    heterotrimeric G-protein complex GO:0005834 IBA
    extracellular exosome GO:0070062 IDA
    endoplasmic reticulum GO:0005783 TAS
 Experiment description of studies that identified GNAZ in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GNAZ
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RGS7  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
2 RAP1GAP 5909
Invitro Homo sapiens
3 ADORA1  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
4 RGS10 6001
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
5 RGS19  
Two-hybrid Homo sapiens
6 OPRD1  
Invitro Homo sapiens
7 EYA2  
Affinity Capture-MS Homo sapiens
8 RGS20 8601
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
9 DRD2  
Invitro Homo sapiens
10 DRD5  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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