Gene description for GNAZ
Gene name guanine nucleotide binding protein (G protein), alpha z polypeptide
Gene symbol GNAZ
Other names/aliases -
Species Homo sapiens
 Database cross references - GNAZ
ExoCarta ExoCarta_2781
Vesiclepedia VP_2781
Entrez Gene 2781
HGNC 4395
MIM 139160
UniProt P19086  
 GNAZ identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for GNAZ
Molecular Function
    G protein-coupled receptor binding GO:0001664 IBA
    GTPase activity GO:0003924 IBA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    adenylate cyclase inhibitor activity GO:0010855 IEA
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    G protein-coupled serotonin receptor binding GO:0031821 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    G protein-coupled receptor signaling pathway GO:0007186 TAS
    adenylate cyclase-modulating G protein-coupled receptor signaling pathway GO:0007188 IBA
    adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway GO:0007193 IEA
    negative regulation of insulin secretion GO:0046676 IEA
    G protein-coupled serotonin receptor signaling pathway GO:0098664 IEA
Subcellular Localization
    nuclear envelope GO:0005635 TAS
    cytoplasm GO:0005737 IBA
    endoplasmic reticulum GO:0005783 TAS
    heterotrimeric G-protein complex GO:0005834 IBA
    plasma membrane GO:0005886 TAS
    dendrite GO:0030425 IEA
    cell body GO:0044297 IEA
    synapse GO:0045202 IEA
 Experiment description of studies that identified GNAZ in exosomes
1
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GNAZ
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 THOC7 80145
Affinity Capture-MS Homo sapiens
2 ATP6V0A1 535
Affinity Capture-MS Homo sapiens
3 SOX2  
Affinity Capture-MS Homo sapiens
4 RNASE3  
Affinity Capture-MS Homo sapiens
5 GNAZ 2781
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
6 BIRC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 RAP1GAP 5909
Reconstituted Complex Homo sapiens
8 GNG10 2790
Affinity Capture-MS Homo sapiens
9 TFCP2 7024
Affinity Capture-MS Homo sapiens
10 RAP1GDS1 5910
Affinity Capture-MS Homo sapiens
11 RNF123 63891
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 MTHFR 4524
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 FBXW5  
Affinity Capture-MS Homo sapiens
14 HBG2 3048
Affinity Capture-MS Homo sapiens
15 EPHA4 2043
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 SDC1 6382
Affinity Capture-MS Homo sapiens
17 GNG3 2785
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 GPM6A 2823
Affinity Capture-MS Homo sapiens
19 RGS19 10287
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
20 HOXB5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 CXCL6  
Affinity Capture-MS Homo sapiens
22 SPG21 51324
Affinity Capture-MS Homo sapiens
23 PCDHGC3 5098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 TUBB8 347688
Affinity Capture-MS Homo sapiens
25 DAAM1 23002
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 EYA2  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
27 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
28 RIC8A 60626
Affinity Capture-MS Homo sapiens
29 PARK2  
Affinity Capture-MS Homo sapiens
30 GNGT1  
Affinity Capture-MS Homo sapiens
31 P2RY8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 SLC25A41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 APP 351
Reconstituted Complex Homo sapiens
34 IFITM1 8519
Affinity Capture-MS Homo sapiens
35 RGS7  
Affinity Capture-Western Homo sapiens
36 GNG2 54331
Affinity Capture-MS Homo sapiens
37 CERS2 29956
Affinity Capture-MS Homo sapiens
38 GJB7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 MED21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 CCNL2 81669
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 APOL2 23780
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 YAP1 10413
Affinity Capture-MS Homo sapiens
43 Htt  
Affinity Capture-MS Mus musculus
44 FAM171A2 284069
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 GLRX3 10539
Affinity Capture-MS Homo sapiens
46 CENPM  
Affinity Capture-MS Homo sapiens
47 RD3  
Affinity Capture-MS Homo sapiens
48 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 DCAF10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 ACBD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 ISG20  
Affinity Capture-MS Homo sapiens
52 GLMP 112770
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 FYTTD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 RGS10 6001
Affinity Capture-Western Homo sapiens
55 ABCE1 6059
Affinity Capture-MS Homo sapiens
56 TM6SF2  
Affinity Capture-MS Homo sapiens
57 GNG8  
Affinity Capture-MS Homo sapiens
58 RGS20 8601
Biochemical Activity Homo sapiens
Co-localization Homo sapiens
59 CRCP  
Affinity Capture-MS Homo sapiens
60 DDX19B 11269
Affinity Capture-MS Homo sapiens
61 DMWD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 TMEM185A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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