Gene description for IFITM1
Gene name interferon induced transmembrane protein 1
Gene symbol IFITM1
Other names/aliases 9-27
CD225
DSPA2a
IFI17
LEU13
Species Homo sapiens
 Database cross references - IFITM1
ExoCarta ExoCarta_8519
Vesiclepedia VP_8519
Entrez Gene 8519
HGNC 5412
MIM 604456
UniProt P13164  
 IFITM1 identified in sEVs derived from the following tissue/cell type
Bladder cancer cells 20224111    
Colorectal cancer cells 19837982    
Colorectal cancer cells 25890246    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Melanoma cells 25950383    
Melanoma cells 25950383    
 Gene ontology annotations for IFITM1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    ossification GO:0001503 IEA
    cell surface receptor signaling pathway GO:0007166 TAS
    negative regulation of cell population proliferation GO:0008285 IMP
    response to virus GO:0009615 IDA
    negative regulation of cell migration GO:0030336 IMP
    response to type II interferon GO:0034341 IBA
    response to type II interferon GO:0034341 IDA
    response to interferon-alpha GO:0035455 IBA
    response to interferon-alpha GO:0035455 IDA
    response to interferon-beta GO:0035456 IBA
    response to interferon-beta GO:0035456 IDA
    negative regulation of viral genome replication GO:0045071 IBA
    negative regulation of viral genome replication GO:0045071 IDA
    negative regulation of viral genome replication GO:0045071 IMP
    positive regulation of osteoblast differentiation GO:0045669 IMP
    negative regulation of viral entry into host cell GO:0046597 IBA
    negative regulation of viral entry into host cell GO:0046597 IDA
    defense response to virus GO:0051607 IBA
    type I interferon-mediated signaling pathway GO:0060337 IBA
Subcellular Localization
    lysosomal membrane GO:0005765 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 HDA
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified IFITM1 in sEVs
1
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 286
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for IFITM1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TMEM170A  
Affinity Capture-MS Homo sapiens
2 SLC43A3 29015
Affinity Capture-MS Homo sapiens
3 GNAZ 2781
Affinity Capture-MS Homo sapiens
4 ABHD16A 7920
Two-hybrid Homo sapiens
5 CD19 930
Affinity Capture-Western Homo sapiens
6 KRTCAP2 200185
Affinity Capture-MS Homo sapiens
7 E2F4  
Affinity Capture-MS Homo sapiens
8 TMEM245 23731
Affinity Capture-MS Homo sapiens
9 NCAM1 4684
Affinity Capture-MS Homo sapiens
10 PRRT2  
Affinity Capture-MS Homo sapiens
11 IFITM3 10410
Affinity Capture-MS Homo sapiens
12 FUNDC2 65991
Affinity Capture-MS Homo sapiens
13 PSME1 5720
Affinity Capture-MS Homo sapiens
14 SLC38A9  
Affinity Capture-MS Homo sapiens
15 PCDH10 57575
Affinity Capture-MS Homo sapiens
16 DNAJC5B  
Affinity Capture-MS Homo sapiens
17 SPPL2B 56928
Affinity Capture-MS Homo sapiens
18 LPAR2 9170
Affinity Capture-MS Homo sapiens
19 CD81 975
Affinity Capture-Western Homo sapiens
20 LTBP1 4052
Affinity Capture-MS Homo sapiens
21 VIPR1  
Affinity Capture-MS Homo sapiens
22 PIGH  
Affinity Capture-MS Homo sapiens
23 CR2 1380
Affinity Capture-Western Homo sapiens
24 RRAGC 64121
Affinity Capture-MS Homo sapiens
25 PCDHB3  
Affinity Capture-MS Homo sapiens
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