Gene description for RNF123
Gene name ring finger protein 123
Gene symbol RNF123
Other names/aliases FP1477
KPC1
Species Homo sapiens
 Database cross references - RNF123
ExoCarta ExoCarta_63891
Vesiclepedia VP_63891
Entrez Gene 63891
HGNC 21148
MIM 614472
UniProt Q5XPI4  
 RNF123 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
 Gene ontology annotations for RNF123
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IBA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    ubiquitin protein ligase activity GO:0061630 IDA
Biological Process
    protein polyubiquitination GO:0000209 IDA
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    proteolysis involved in protein catabolic process GO:0051603 IBA
    protein maturation GO:0051604 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    nuclear membrane GO:0031965 IDA
 Experiment description of studies that identified RNF123 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RNF123
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAP3K6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 A1BG 1
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 FAS 355
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
4 RNASE3  
Affinity Capture-MS Homo sapiens
5 UBE2W  
Two-hybrid Homo sapiens
6 Tor1aip1  
Affinity Capture-MS Mus musculus
7 ITGA5 3678
Affinity Capture-MS Homo sapiens
8 DEFB125  
Affinity Capture-MS Homo sapiens
9 FUK 197258
Affinity Capture-MS Homo sapiens
10 D2HGDH 728294
Affinity Capture-MS Homo sapiens
11 UBAC1 10422
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
12 KIF21A 55605
Affinity Capture-MS Homo sapiens
13 CUL3 8452
Affinity Capture-MS Homo sapiens
14 DVL2 1856
Affinity Capture-Western Homo sapiens
15 PMS1  
Affinity Capture-Western Homo sapiens
16 DDX58 23586
Affinity Capture-RNA Homo sapiens
17 INSL6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 USP48  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 C3orf58 205428
Affinity Capture-MS Homo sapiens
20 Rab5b 19344
Affinity Capture-MS Mus musculus
21 LINC01565  
Affinity Capture-MS Homo sapiens
22 LCMT2 9836
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 GNAZ 2781
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 RDH12 145226
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 ZNF277  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 FUOM  
Affinity Capture-MS Homo sapiens
27 PNPLA1 285848
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 SULT1C4  
Affinity Capture-MS Homo sapiens
29 SPINK14  
Affinity Capture-MS Homo sapiens
30 RPL10 6134
Affinity Capture-MS Homo sapiens
31 NR3C1 2908
Proximity Label-MS Homo sapiens
32 EFNA4  
Affinity Capture-MS Homo sapiens
33 UBC 7316
Reconstituted Complex Homo sapiens
34 CHIA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 UBE2D1 7321
Affinity Capture-MS Homo sapiens
36 ELAC2 60528
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 GADD45GIP1  
Affinity Capture-MS Homo sapiens
38 RPS2P32  
Affinity Capture-MS Homo sapiens
39 METTL21B  
Affinity Capture-MS Homo sapiens
40 NMB  
Affinity Capture-MS Homo sapiens
41 HYAL1 3373
Affinity Capture-MS Homo sapiens
42 KLHL11  
Affinity Capture-MS Homo sapiens
43 NXF1 10482
Affinity Capture-RNA Homo sapiens
44 BAG6 7917
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
45 BVES  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 PLOD2 5352
Co-fractionation Homo sapiens
47 NSMF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 C12orf74  
Affinity Capture-MS Homo sapiens
49 MOV10 4343
Affinity Capture-RNA Homo sapiens
50 CHMP4B 128866
Affinity Capture-MS Homo sapiens
51 INF2 64423
Affinity Capture-MS Homo sapiens
52 SMAD9  
Two-hybrid Homo sapiens
53 DHFR 1719
Affinity Capture-MS Homo sapiens
54 INSL3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 SPRTN  
Affinity Capture-MS Homo sapiens
56 LECT2  
Affinity Capture-MS Homo sapiens
57 AKIRIN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 PCDHB15  
Affinity Capture-MS Homo sapiens
59 ALDH3B1 221
Affinity Capture-MS Homo sapiens
60 NFKB1 4790
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
61 SMAD2 4087
Two-hybrid Homo sapiens
62 PML 5371
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 SLC27A2 11001
Affinity Capture-MS Homo sapiens
64 NDUFA10 4705
Affinity Capture-MS Homo sapiens
65 REG4 83998
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 FTSJ2 29960
Affinity Capture-MS Homo sapiens
67 CDKN1B 1027
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
68 UBE2D2 7322
Reconstituted Complex Homo sapiens
69 FBXW5  
Affinity Capture-MS Homo sapiens
70 NMS  
Affinity Capture-MS Homo sapiens
71 MRPS14  
Affinity Capture-MS Homo sapiens
72 IGF2 3481
Affinity Capture-MS Homo sapiens
73 RAD21 5885
Affinity Capture-Western Homo sapiens
74 GREM2 64388
Affinity Capture-MS Homo sapiens
75 TACC3  
Affinity Capture-MS Homo sapiens
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