Gene description for RDH12
Gene name retinol dehydrogenase 12 (all-trans/9-cis/11-cis)
Gene symbol RDH12
Other names/aliases LCA13
LCA3
RP53
SDR7C2
Species Homo sapiens
 Database cross references - RDH12
ExoCarta ExoCarta_145226
Vesiclepedia VP_145226
Entrez Gene 145226
HGNC 19977
MIM 608830
UniProt Q96NR8  
 RDH12 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for RDH12
Molecular Function
    all-trans-retinol dehydrogenase (NAD+) activity GO:0004745 IDA
    protein binding GO:0005515 IPI
    all-trans-retinol dehydrogenase (NADP+) activity GO:0052650 IDA
    all-trans-retinol dehydrogenase (NADP+) activity GO:0052650 TAS
    11-cis-retinol dehydrogenase activity GO:0102354 IEA
Biological Process
    retinoid metabolic process GO:0001523 TAS
    visual perception GO:0007601 IMP
    visual perception GO:0007601 TAS
    retinol metabolic process GO:0042572 IDA
    retinol metabolic process GO:0042572 IEA
    photoreceptor cell maintenance GO:0045494 TAS
    cellular detoxification of aldehyde GO:0110095 IDA
Subcellular Localization
    photoreceptor inner segment GO:0001917 ISS
    endoplasmic reticulum membrane GO:0005789 IEA
    photoreceptor inner segment membrane GO:0060342 TAS
 Experiment description of studies that identified RDH12 in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RDH12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDXP 57026
Affinity Capture-MS Homo sapiens
2 TSPAN5 10098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 RNF123 63891
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 C18orf21  
Affinity Capture-MS Homo sapiens
5 TTYH1  
Affinity Capture-MS Homo sapiens
6 STAMBP 10617
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 TES 26136
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 AGPAT1 10554
Affinity Capture-MS Homo sapiens
9 BUB1  
Two-hybrid Homo sapiens
10 PEX19 5824
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 ANXA8L1 728113
Two-hybrid Homo sapiens
12 UBE2E3 10477
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 AGFG1 3267
Affinity Capture-MS Homo sapiens
14 NME2P1 283458
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 TSPAN15 23555
Affinity Capture-MS Homo sapiens
16 UBE2T  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 ZC3HC1  
Affinity Capture-MS Homo sapiens
18 RANBP3 8498
Affinity Capture-MS Homo sapiens
19 EIF4EBP2 1979
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 UBAC1 10422
Affinity Capture-MS Homo sapiens
21 EFHD2 79180
Affinity Capture-MS Homo sapiens
22 UBE2D2 7322
Affinity Capture-MS Homo sapiens
23 MBNL1 4154
Affinity Capture-MS Homo sapiens
24 MAP1LC3A 84557
Two-hybrid Homo sapiens
25 PMVK 10654
Affinity Capture-MS Homo sapiens
26 PINK1  
Affinity Capture-MS Homo sapiens
27 PLEKHA7 144100
Two-hybrid Homo sapiens
28 RDH11 51109
Cross-Linking-MS (XL-MS) Homo sapiens
29 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
30 SSUH2  
Affinity Capture-MS Homo sapiens
31 LASP1 3927
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 OR2A4 79541
Affinity Capture-MS Homo sapiens
33 ZBTB8OS 339487
Affinity Capture-MS Homo sapiens
34 TKFC 26007
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 CTH 1491
Affinity Capture-MS Homo sapiens
36 ARPIN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 CCR1  
Affinity Capture-MS Homo sapiens
38 RBPMS 11030
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
39 STX17 55014
Affinity Capture-MS Homo sapiens
40 CHMP4A 29082
Affinity Capture-MS Homo sapiens
41 ARFGAP1 55738
Affinity Capture-MS Homo sapiens
42 CCDC25 55246
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here