Gene description for ARFGAP1
Gene name ADP-ribosylation factor GTPase activating protein 1
Gene symbol ARFGAP1
Other names/aliases ARF1GAP
HRIHFB2281
Species Homo sapiens
 Database cross references - ARFGAP1
ExoCarta ExoCarta_55738
Vesiclepedia VP_55738
Entrez Gene 55738
HGNC 15852
MIM 608377
UniProt Q8N6T3  
 ARFGAP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for ARFGAP1
Molecular Function
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 IDA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    protein transport GO:0015031 IEA
    vesicle-mediated transport GO:0016192 IEA
    regulation of endocytosis GO:0030100 IBA
    regulation of ARF protein signal transduction GO:0032012 IBA
Subcellular Localization
    Golgi membrane GO:0000139 IBA
    cytosol GO:0005829 TAS
    synapse GO:0045202 IDA
 Experiment description of studies that identified ARFGAP1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ARFGAP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AP2S1 1175
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 COPG2 26958
Affinity Capture-MS Homo sapiens
3 AP2B1 163
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 TK1 7083
Two-hybrid Homo sapiens
5 SPRED2 200734
Affinity Capture-MS Homo sapiens
6 ARFGAP2 84364
Co-fractionation Homo sapiens
7 TMEM206 55248
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 ARFGAP3 26286
Co-fractionation Homo sapiens
9 HRAS 3265
Reconstituted Complex Homo sapiens
10 APOE 348
Two-hybrid Homo sapiens
11 RIC8A 60626
Affinity Capture-MS Homo sapiens
12 SPAG9 9043
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 COPA 1314
Reconstituted Complex Homo sapiens
14 APP 351
Reconstituted Complex Homo sapiens
15 KDELR1 10945
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
16 POTEF 728378
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 TCEA1 6917
Co-fractionation Homo sapiens
18 MAP1LC3B 81631
Two-hybrid Homo sapiens
19 LASP1 3927
Co-fractionation Homo sapiens
20 DYNC1LI2 1783
Proximity Label-MS Homo sapiens
21 APOC3 345
Two-hybrid Homo sapiens
22 GSTM5 2949
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 AP2A1 160
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 KRTAP9-2  
Two-hybrid Homo sapiens
25 COPS5 10987
Two-hybrid Homo sapiens
26 RFC3 5983
Two-hybrid Homo sapiens
27 STAT3 6774
Proximity Label-MS Homo sapiens
28 MED27  
Two-hybrid Homo sapiens
29 AGTR1  
Two-hybrid Homo sapiens
30 CRMP1 1400
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 UFSP2 55325
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 HK1 3098
Co-fractionation Homo sapiens
33 TFRC 7037
Affinity Capture-Western Homo sapiens
34 AURKA 6790
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 RDH12 145226
Affinity Capture-MS Homo sapiens
36 RGS2  
Two-hybrid Homo sapiens
37 CARM1 10498
Affinity Capture-MS Homo sapiens
38 LAMP1 3916
Affinity Capture-MS Homo sapiens
39 AP1B1 162
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 PYCRL 65263
Affinity Capture-MS Homo sapiens
41 ASB10  
Two-hybrid Homo sapiens
42 RPA4  
Proximity Label-MS Homo sapiens
43 STX5 6811
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 SND1 27044
Affinity Capture-MS Homo sapiens
45 PRKACB 5567
Two-hybrid Homo sapiens
46 CD2AP 23607
Affinity Capture-MS Homo sapiens
47 RPL11 6135
Two-hybrid Homo sapiens
48 REEP5 7905
Two-hybrid Homo sapiens
49 PHLPP1  
Proximity Label-MS Homo sapiens
50 SEC63 11231
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 DIP2B 57609
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 XPO1 7514
Affinity Capture-MS Homo sapiens
53 APOB 338
Two-hybrid Homo sapiens
54 RASIP1  
Two-hybrid Homo sapiens
55 FNTA 2339
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 AP2M1 1173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 MAU2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 FOS 2353
Two-hybrid Homo sapiens
59 RPA3 6119
Proximity Label-MS Homo sapiens
60 AP2A2 161
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 NIPBL 25836
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 RPL22 6146
Co-fractionation Homo sapiens
63 TGFA  
Affinity Capture-MS Homo sapiens
64 BICD1 636
Proximity Label-MS Homo sapiens
65 FNTB 2342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 PARP1 142
Proximity Label-MS Homo sapiens
67 KRTAP3-2  
Two-hybrid Homo sapiens
68 MIRLET7A1  
Affinity Capture-RNA Homo sapiens
69 FAM20C 56975
Affinity Capture-MS Homo sapiens
70 RNF43  
Proximity Label-MS Homo sapiens
71 GRN 2896
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
72 SMN1 6606
Two-hybrid Homo sapiens
73 DLG3 1741
Co-fractionation Homo sapiens
74 TGFBRAP1 9392
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 INPPL1 3636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 GABARAPL2 11345
Two-hybrid Homo sapiens
77 FGB 2244
Affinity Capture-MS Homo sapiens
78 SQSTM1 8878
Proximity Label-MS Homo sapiens
79 SLC6A1  
Reconstituted Complex Homo sapiens
80 LRRK2 120892
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
81 TRIM23 373
Two-hybrid Homo sapiens
82 TRIM31  
Affinity Capture-MS Homo sapiens
83 ARF1 375
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
84 SERBP1 26135
Affinity Capture-MS Homo sapiens
85 CCDC96  
Affinity Capture-MS Homo sapiens
86 RAB23 51715
Two-hybrid Homo sapiens
87 KRTAP10-9  
Two-hybrid Homo sapiens
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