Gene description for ARFGAP3
Gene name ADP-ribosylation factor GTPase activating protein 3
Gene symbol ARFGAP3
Other names/aliases ARFGAP1
Species Homo sapiens
 Database cross references - ARFGAP3
ExoCarta ExoCarta_26286
Entrez Gene 26286
HGNC 661
MIM 612439
UniProt Q9NP61  
 ARFGAP3 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for ARFGAP3
Molecular Function
    protein transporter activity GO:0008565 NAS
    GTPase activator activity GO:0005096 IDA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    vesicle-mediated transport GO:0016192 NAS
    intracellular protein transport GO:0006886 NAS
    positive regulation of GTPase activity GO:0043547 IEA
    protein secretion GO:0009306 IEP
Subcellular Localization
    Golgi apparatus GO:0005794 IDA
    Golgi membrane GO:0000139 IEA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    membrane GO:0016020 IDA
 Experiment description of studies that identified ARFGAP3 in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for ARFGAP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ARFGAP3 is involved
PathwayEvidenceSource
COPI Mediated Transport TAS Reactome





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