Gene description for ARFGAP3
Gene name ADP-ribosylation factor GTPase activating protein 3
Gene symbol ARFGAP3
Other names/aliases ARFGAP1
Species Homo sapiens
 Database cross references - ARFGAP3
ExoCarta ExoCarta_26286
Vesiclepedia VP_26286
Entrez Gene 26286
HGNC 661
MIM 612439
UniProt Q9NP61  
 ARFGAP3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for ARFGAP3
Molecular Function
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 IDA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    intracellular protein transport GO:0006886 NAS
    protein secretion GO:0009306 IEP
    vesicle-mediated transport GO:0016192 NAS
    COPI coating of Golgi vesicle GO:0048205 IBA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified ARFGAP3 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for ARFGAP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD14 929
Two-hybrid Homo sapiens
2 RNF43  
Proximity Label-MS Homo sapiens
3 GJD3  
Proximity Label-MS Homo sapiens
4 SHPK 23729
Affinity Capture-MS Homo sapiens
5 CXCL8 3576
Two-hybrid Homo sapiens
6 TPPP3 51673
Co-fractionation Homo sapiens
7 EBAG9 9166
Proximity Label-MS Homo sapiens
8 RPN2 6185
Proximity Label-MS Homo sapiens
9 METTL7A 25840
Proximity Label-MS Homo sapiens
10 LAMTOR1 55004
Proximity Label-MS Homo sapiens
11 MARCKS 4082
Proximity Label-MS Homo sapiens
12 LAMP3  
Proximity Label-MS Homo sapiens
13 XPO1 7514
Affinity Capture-MS Homo sapiens
14 PANX1 24145
Proximity Label-MS Homo sapiens
15 GJA1 2697
Proximity Label-MS Homo sapiens
16 SNAPC4  
Affinity Capture-MS Homo sapiens
17 PXMP2  
Proximity Label-MS Homo sapiens
18 PLEC 5339
Affinity Capture-MS Homo sapiens
19 RAB5A 5868
Proximity Label-MS Homo sapiens
20 ZDHHC17 23390
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
21 ARF5 381
Proximity Label-MS Homo sapiens
22 S100A2 6273
Affinity Capture-MS Homo sapiens
23 HSD3B7 80270
Proximity Label-MS Homo sapiens
24 ARF3 377
Proximity Label-MS Homo sapiens
25 ADI1 55256
Co-fractionation Homo sapiens
26 TRIM26 7726
Affinity Capture-MS Homo sapiens
27 HSD17B11 51170
Proximity Label-MS Homo sapiens
28 EMD 2010
Proximity Label-MS Homo sapiens
29 FKBP8 23770
Proximity Label-MS Homo sapiens
30 HSD17B10 3028
Co-fractionation Homo sapiens
31 RHOT2 89941
Proximity Label-MS Homo sapiens
32 ANKRD36B  
Affinity Capture-MS Homo sapiens
33 ERGIC1 57222
Proximity Label-MS Homo sapiens
34 DUSP16  
Affinity Capture-MS Homo sapiens
35 CCDC102B  
Affinity Capture-MS Homo sapiens
36 APP 351
Reconstituted Complex Homo sapiens
37 SQSTM1 8878
Proximity Label-MS Homo sapiens
38 PPIP5K2 23262
Affinity Capture-MS Homo sapiens
39 RAB4A 5867
Proximity Label-MS Homo sapiens
40 ARF4 378
Proximity Label-MS Homo sapiens
41 CAPZB 832
Affinity Capture-MS Homo sapiens
42 ERGIC2 51290
Proximity Label-MS Homo sapiens
43 MAPK6  
Affinity Capture-MS Homo sapiens
44 B3GAT1  
Proximity Label-MS Homo sapiens
45 ARFGAP2 84364
Affinity Capture-MS Homo sapiens
46 SEC62 7095
Proximity Label-MS Homo sapiens
47 ATP2A1 487
Proximity Label-MS Homo sapiens
48 RTN4IP1  
Two-hybrid Homo sapiens
49 RAB11A 8766
Proximity Label-MS Homo sapiens
50 ARF1 375
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
51 HSPA1A 3303
Affinity Capture-MS Homo sapiens
52 RAB3B 5865
Proximity Label-MS Homo sapiens
53 BICD2 23299
Proximity Label-MS Homo sapiens
54 BICD1 636
Proximity Label-MS Homo sapiens
55 LIME1 54923
Two-hybrid Homo sapiens
56 STX4 6810
Proximity Label-MS Homo sapiens
57 CYP2C9  
Proximity Label-MS Homo sapiens
58 CCDC96  
Affinity Capture-MS Homo sapiens
59 RPA3 6119
Proximity Label-MS Homo sapiens
60 CAV1 857
Proximity Label-MS Homo sapiens
61 ELOVL5 60481
Proximity Label-MS Homo sapiens
62 SEC61B 10952
Proximity Label-MS Homo sapiens
63 STX6 10228
Proximity Label-MS Homo sapiens
64 ARFGAP1 55738
Co-fractionation Homo sapiens
65 DERL1 79139
Proximity Label-MS Homo sapiens
66 RAB9A 9367
Proximity Label-MS Homo sapiens
67 WDYHV1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here