Gene description for TPPP3
Gene name tubulin polymerization-promoting protein family member 3
Gene symbol TPPP3
Other names/aliases CGI-38
TPPP/p20
p20
p25gamma
Species Homo sapiens
 Database cross references - TPPP3
ExoCarta ExoCarta_51673
Vesiclepedia VP_51673
Entrez Gene 51673
HGNC 24162
UniProt Q9BW30  
 TPPP3 identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for TPPP3
Molecular Function
    tubulin binding GO:0015631 IBA
    tubulin binding GO:0015631 IDA
Biological Process
    microtubule bundle formation GO:0001578 IBA
    microtubule bundle formation GO:0001578 IDA
    embryo implantation GO:0007566 ISS
    positive regulation of protein polymerization GO:0032273 IBA
    decidualization GO:0046697 IMP
    microtubule polymerization GO:0046785 IBA
Subcellular Localization
    microtubule GO:0005874 IBA
    perinuclear region of cytoplasm GO:0048471 IDA
    microtubule bundle GO:0097427 IDA
 Experiment description of studies that identified TPPP3 in exosomes
1
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for TPPP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ARFGAP3 26286
Co-fractionation Homo sapiens
2 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
3 SREBF2 6721
Positive Genetic Homo sapiens
4 ZC3HC1  
Affinity Capture-MS Homo sapiens
5 GOT1 2805
Affinity Capture-MS Homo sapiens
6 Htatsf1  
Two-hybrid Mus musculus
View the network image/svg+xml
 Pathways in which TPPP3 is involved
No pathways found





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