Gene description for HK1
Gene name hexokinase 1
Gene symbol HK1
Other names/aliases HK1-ta
HK1-tb
HK1-tc
HKD
HKI
HMSNR
HXK1
Species Homo sapiens
 Database cross references - HK1
ExoCarta ExoCarta_3098
Entrez Gene 3098
HGNC 4922
MIM 142600
UniProt P19367  
 HK1 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for HK1
Molecular Function
    mannokinase activity GO:0019158 IBA
    fructokinase activity GO:0008865 IBA
    protein binding GO:0005515 IPI
    glucokinase activity GO:0004340 IBA
    hexokinase activity GO:0004396 TAS
    ATP binding GO:0005524 IEA
Biological Process
    glucose metabolic process GO:0006006 TAS
    transmembrane transport GO:0055085 TAS
    carbohydrate metabolic process GO:0005975 TAS
    canonical glycolysis GO:0061621 TAS
    small molecule metabolic process GO:0044281 TAS
    glucose transport GO:0015758 TAS
    cellular glucose homeostasis GO:0001678 IBA
    glucose 6-phosphate metabolic process GO:0051156 IBA
    glycolytic process GO:0006096 TAS
    carbohydrate phosphorylation GO:0046835 TAS
    hexose transport GO:0008645 TAS
Subcellular Localization
    mitochondrion GO:0005739 IDA
    cytosol GO:0005829 TAS
    sperm principal piece GO:0097228 IEA
    membrane raft GO:0045121 IEA
    mitochondrial outer membrane GO:0005741 IEA
 Experiment description of studies that identified HK1 in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VDAC1 7416
Affinity Capture-MS Homo sapiens
2 ENO2 2026
Two-hybrid Homo sapiens
3 VDAC2 7417
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which HK1 is involved
PathwayEvidenceSource
Glucose transport TAS Reactome
Glycolysis TAS Reactome





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