Gene description for UBA5
Gene name ubiquitin-like modifier activating enzyme 5
Gene symbol UBA5
Other names/aliases THIFP1
UBE1DC1
Species Homo sapiens
 Database cross references - UBA5
ExoCarta ExoCarta_79876
Vesiclepedia VP_79876
Entrez Gene 79876
HGNC 23230
MIM 610552
UniProt Q9GZZ9  
 UBA5 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for UBA5
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    zinc ion binding GO:0008270 IDA
    protein homodimerization activity GO:0042803 IDA
    UFM1 activating enzyme activity GO:0071566 IBA
    UFM1 activating enzyme activity GO:0071566 IDA
    UFM1 activating enzyme activity GO:0071566 IMP
Biological Process
    erythrocyte differentiation GO:0030218 ISS
    megakaryocyte differentiation GO:0030219 ISS
    regulation of type II interferon production GO:0032649 IEA
    regulation of intracellular estrogen receptor signaling pathway GO:0033146 IMP
    response to endoplasmic reticulum stress GO:0034976 IDA
    response to endoplasmic reticulum stress GO:0034976 IMP
    neuromuscular process GO:0050905 IGI
    reticulophagy GO:0061709 IMP
    protein ufmylation GO:0071569 IBA
    protein ufmylation GO:0071569 IDA
    protein ufmylation GO:0071569 IMP
    protein ufmylation GO:0071569 IMP
    protein K69-linked ufmylation GO:1990592 IDA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum membrane GO:0005789 IDA
    Golgi apparatus GO:0005794 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified UBA5 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for UBA5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBS 875
Co-fractionation Homo sapiens
2 EIF3A 8661
Affinity Capture-MS Homo sapiens
3 ENSA 2029
Affinity Capture-MS Homo sapiens
4 PKD1P1 339044
Two-hybrid Homo sapiens
5 DDX17 10521
Affinity Capture-MS Homo sapiens
6 MRPL17  
Affinity Capture-MS Homo sapiens
7 USP5 8078
Co-fractionation Homo sapiens
8 BOLL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 ALDH2 217
Affinity Capture-MS Homo sapiens
10 GAPDHS 26330
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 NTNG1  
Affinity Capture-MS Homo sapiens
12 CYTH1 9267
Affinity Capture-MS Homo sapiens
13 APEX1 328
Affinity Capture-RNA Homo sapiens
14 PECR 55825
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 PGK1 5230
Affinity Capture-MS Homo sapiens
16 PRDX3 10935
Affinity Capture-MS Homo sapiens
17 MCM2 4171
Affinity Capture-MS Homo sapiens
18 UBE2K 3093
Reconstituted Complex Homo sapiens
19 COPB2 9276
Affinity Capture-MS Homo sapiens
20 PGM1 5236
Affinity Capture-MS Homo sapiens
21 THTPA  
Co-fractionation Homo sapiens
22 ZNF512B  
Affinity Capture-MS Homo sapiens
23 ETFA 2108
Affinity Capture-MS Homo sapiens
24 IPP  
Affinity Capture-MS Homo sapiens
25 LTA4H 4048
Affinity Capture-MS Homo sapiens
26 LOC81691 81691
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 CRMP1 1400
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 NUDCD1 84955
Co-fractionation Homo sapiens
29 NSFL1C 55968
Co-fractionation Homo sapiens
30 HSD17B4 3295
Affinity Capture-MS Homo sapiens
31 GSTO1 9446
Affinity Capture-MS Homo sapiens
32 TMEM132D  
Two-hybrid Homo sapiens
33 HK1 3098
Co-fractionation Homo sapiens
34 CBR1 873
Affinity Capture-MS Homo sapiens
35 HN1L 90861
Affinity Capture-MS Homo sapiens
36 S100A2 6273
Affinity Capture-MS Homo sapiens
37 NAE1 8883
Co-fractionation Homo sapiens
38 P4HA1 5033
Affinity Capture-MS Homo sapiens
39 DBNL 28988
Co-fractionation Homo sapiens
40 HSP90B1 7184
Affinity Capture-MS Homo sapiens
41 C12orf49  
Positive Genetic Homo sapiens
42 PSMD2 5708
Affinity Capture-MS Homo sapiens
43 VCP 7415
Affinity Capture-MS Homo sapiens
44 OGT 8473
Reconstituted Complex Homo sapiens
45 LAP3 51056
Affinity Capture-MS Homo sapiens
46 PARP6  
Co-fractionation Homo sapiens
47 MOGS 7841
Affinity Capture-MS Homo sapiens
48 GANAB 23193
Affinity Capture-MS Homo sapiens
49 MZT2A  
Affinity Capture-MS Homo sapiens
50 ADAMTS1 9510
Affinity Capture-MS Homo sapiens
51 SND1 27044
Affinity Capture-MS Homo sapiens
52 CARTPT  
Affinity Capture-MS Homo sapiens
53 SNAP29 9342
Affinity Capture-MS Homo sapiens
54 C1QTNF2  
Affinity Capture-MS Homo sapiens
55 ALDH7A1 501
Affinity Capture-MS Homo sapiens
56 RPL32 6161
Affinity Capture-MS Homo sapiens
57 UFC1 51506
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 XPO1 7514
Affinity Capture-MS Homo sapiens
59 NCBP2 22916
Affinity Capture-MS Homo sapiens
60 HSPE1 3336
Affinity Capture-MS Homo sapiens
61 UBE2L3 7332
Reconstituted Complex Homo sapiens
62 NAA15 80155
Affinity Capture-MS Homo sapiens
63 ATP6V1D 51382
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 VAMP3 9341
Affinity Capture-MS Homo sapiens
65 EGFR 1956
Negative Genetic Homo sapiens
66 ANP32E 81611
Affinity Capture-MS Homo sapiens
67 ECHS1 1892
Affinity Capture-MS Homo sapiens
68 DUSP13  
Affinity Capture-MS Homo sapiens
69 FARSB 10056
Affinity Capture-MS Homo sapiens
70 MREG  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 PSPC1 55269
Affinity Capture-MS Homo sapiens
72 SERBP1 26135
Affinity Capture-MS Homo sapiens
73 APEH 327
Two-hybrid Homo sapiens
74 GOLGA1  
Affinity Capture-MS Homo sapiens
75 PLIN3 10226
Co-fractionation Homo sapiens
76 TBL1X 6907
Affinity Capture-MS Homo sapiens
77 TP53RK 112858
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 SH3GLB2 56904
Affinity Capture-MS Homo sapiens
79 SARS 6301
Affinity Capture-MS Homo sapiens
80 CDC20B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 RPS16 6217
Affinity Capture-MS Homo sapiens
82 PA2G4 5036
Affinity Capture-MS Homo sapiens
83 FGL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 EIF2S1 1965
Co-fractionation Homo sapiens
85 FASN 2194
Negative Genetic Homo sapiens
Affinity Capture-MS Homo sapiens
86 CCT8 10694
Co-fractionation Homo sapiens
87 UBA5 79876
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
88 KLHL8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 PDCD6IP 10015
Affinity Capture-MS Homo sapiens
90 HADH 3033
Affinity Capture-MS Homo sapiens
91 GABARAPL2 11345
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 ATG101  
Affinity Capture-MS Homo sapiens
93 PPP1R9B 84687
Affinity Capture-MS Homo sapiens
94 FKBP4 2288
Affinity Capture-MS Homo sapiens
95 PNMA1 9240
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 RNF20 56254
Affinity Capture-MS Homo sapiens
97 SHMT2 6472
Affinity Capture-MS Homo sapiens
98 UBE2D2 7322
Reconstituted Complex Homo sapiens
99 TARS 6897
Affinity Capture-MS Homo sapiens
100 SFXN1 94081
Affinity Capture-MS Homo sapiens
101 DDX39A 10212
Affinity Capture-MS Homo sapiens
102 RPS28 6234
Affinity Capture-MS Homo sapiens
103 C10orf88  
Affinity Capture-MS Homo sapiens
104 ALYREF 10189
Co-fractionation Homo sapiens
105 ACOX1 51
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 YBX3 8531
Affinity Capture-MS Homo sapiens
107 PRKCA 5578
Affinity Capture-MS Homo sapiens
108 LDLR 3949
Negative Genetic Homo sapiens
109 SNW1 22938
Affinity Capture-MS Homo sapiens
110 KRAS 3845
Negative Genetic Homo sapiens
111 UFM1 51569
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 EP300 2033
Affinity Capture-MS Homo sapiens
113 CDC5L 988
Affinity Capture-MS Homo sapiens
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