Gene description for UBA5
Gene name ubiquitin-like modifier activating enzyme 5
Gene symbol UBA5
Other names/aliases THIFP1
UBE1DC1
Species Homo sapiens
 Database cross references - UBA5
ExoCarta ExoCarta_79876
Entrez Gene 79876
HGNC 23230
MIM 610552
UniProt Q9GZZ9  
 UBA5 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for UBA5
Molecular Function
    ATP binding GO:0005524 IEA
    UFM1 activating enzyme activity GO:0071566 IDA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    protein ufmylation GO:0071569 IMP
    protein K69-linked ufmylation GO:1990592 IDA
    regulation of intracellular estrogen receptor signaling pathway GO:0033146 IMP
    response to endoplasmic reticulum stress GO:0034976 IDA
Subcellular Localization
    intracellular membrane-bounded organelle GO:0043231 IDA
    nucleus GO:0005634 IEA
    cytosol GO:0005829 IBA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified UBA5 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for UBA5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APEH 327
Two-hybrid Homo sapiens
2 GABARAPL2 11345
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which UBA5 is involved
PathwayEvidenceSource
Antigen processing: Ubiquitination & Proteasome degradation TAS Reactome





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