Gene description for NCBP2
Gene name nuclear cap binding protein subunit 2, 20kDa
Gene symbol NCBP2
Other names/aliases CBC2
CBP20
NIP1
PIG55
Species Homo sapiens
 Database cross references - NCBP2
ExoCarta ExoCarta_22916
Vesiclepedia VP_22916
Entrez Gene 22916
HGNC 7659
MIM 605133
UniProt P52298  
 NCBP2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for NCBP2
Molecular Function
    RNA cap binding GO:0000339 IBA
    RNA cap binding GO:0000339 NAS
    RNA 7-methylguanosine cap binding GO:0000340 IDA
    RNA 7-methylguanosine cap binding GO:0000340 IMP
    DNA binding GO:0003677 EXP
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IDA
    mRNA binding GO:0003729 IDA
    protein binding GO:0005515 IPI
    snRNA binding GO:0017069 IDA
Biological Process
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 IDA
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 IMP
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 NAS
    alternative mRNA splicing, via spliceosome GO:0000380 NAS
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 NAS
    cap-dependent translational initiation GO:0002191 NAS
    mRNA export from nucleus GO:0006406 IPI
    snRNA export from nucleus GO:0006408 ISS
    snRNA export from nucleus GO:0006408 NAS
    regulation of translational initiation GO:0006446 IDA
    histone mRNA metabolic process GO:0008334 IMP
    RNA splicing GO:0008380 ISS
    mRNA metabolic process GO:0016071 IMP
    primary miRNA processing GO:0031053 NAS
    mRNA 3'-end processing GO:0031124 NAS
    positive regulation of mRNA 3'-end processing GO:0031442 IMP
    positive regulation of transcription elongation by RNA polymerase II GO:0032968 NAS
    regulatory ncRNA-mediated post-transcriptional gene silencing GO:0035194 NAS
    miRNA-mediated post-transcriptional gene silencing GO:0035195 NAS
    mRNA transcription by RNA polymerase II GO:0042789 IDA
    mRNA cis splicing, via spliceosome GO:0045292 IDA
    positive regulation of RNA export from nucleus GO:0046833 ISS
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 NAS
    cytosol GO:0005829 TAS
    nuclear cap binding complex GO:0005846 IBA
    nuclear cap binding complex GO:0005846 IDA
    nuclear cap binding complex GO:0005846 IPI
    RNA cap binding complex GO:0034518 IMP
 Experiment description of studies that identified NCBP2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NCBP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATG16L1 55054
Affinity Capture-MS Homo sapiens
2 KPNA6 23633
Affinity Capture-MS Homo sapiens
3 PPRC1  
Affinity Capture-MS Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 MYH7B  
Affinity Capture-MS Homo sapiens
6 SNCA 6622
Affinity Capture-MS Homo sapiens
7 CUL1 8454
Affinity Capture-MS Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 USP48  
Co-fractionation Homo sapiens
10 NCBP1 4686
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-RNA Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-RNA Homo sapiens
Affinity Capture-MS Homo sapiens
11 RBM39 9584
Affinity Capture-MS Homo sapiens
12 Tubg1 103733
Affinity Capture-MS Mus musculus
13 KPNA1 3836
Affinity Capture-MS Homo sapiens
14 PRPF4B 8899
Affinity Capture-MS Homo sapiens
15 Sod1 20655
Affinity Capture-MS Mus musculus
16 TOP1 7150
Affinity Capture-MS Homo sapiens
17 CPSF6 11052
Affinity Capture-MS Homo sapiens
18 KIF14 9928
Affinity Capture-MS Homo sapiens
19 UBA5 79876
Affinity Capture-MS Homo sapiens
20 XPO1 7514
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
21 LIN28A  
Affinity Capture-MS Homo sapiens
22 RC3H2  
Affinity Capture-MS Homo sapiens
23 RNMT 8731
Co-fractionation Homo sapiens
24 SNRPB 6628
Affinity Capture-MS Homo sapiens
25 AURKA 6790
Affinity Capture-MS Homo sapiens
26 ELL  
Affinity Capture-MS Homo sapiens
27 TARSL2 123283
Affinity Capture-MS Homo sapiens
28 SNRPA 6626
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 RPL10 6134
Affinity Capture-MS Homo sapiens
30 CBR1 873
Affinity Capture-MS Homo sapiens
31 DDX21 9188
Affinity Capture-MS Homo sapiens
32 LIN28B  
Affinity Capture-MS Homo sapiens
33 ZCCHC8 55596
Affinity Capture-MS Homo sapiens
34 KPNA2 3838
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 CAND1 55832
Affinity Capture-MS Homo sapiens
36 EIF4E 1977
Co-fractionation Homo sapiens
37 PHAX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 EIF4A3 9775
Co-fractionation Homo sapiens
39 SUPT5H 6829
Affinity Capture-MS Homo sapiens
40 SNRPF 6636
Affinity Capture-MS Homo sapiens
41 NUP98 4928
Affinity Capture-MS Homo sapiens
42 SOX2  
Affinity Capture-MS Homo sapiens
43 RBM7  
Affinity Capture-MS Homo sapiens
44 ICE1  
Affinity Capture-MS Homo sapiens
45 NCBP2-AS2  
Affinity Capture-MS Homo sapiens
46 NCBP2 22916
Affinity Capture-RNA Homo sapiens
Affinity Capture-RNA Homo sapiens
47 SF3A1 10291
Affinity Capture-MS Homo sapiens
48 ZBTB48  
Affinity Capture-MS Homo sapiens
49 LZTS2 84445
Two-hybrid Homo sapiens
50 FASN 2194
Positive Genetic Homo sapiens
51 NUP50 10762
Affinity Capture-MS Homo sapiens
52 ADPRHL2 54936
Co-fractionation Homo sapiens
53 E2F4  
Affinity Capture-MS Homo sapiens
54 RBM42  
Affinity Capture-MS Homo sapiens
55 MYC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 CTIF  
Co-fractionation Homo sapiens
57 STX3 6809
Affinity Capture-MS Homo sapiens
58 RBM3 5935
Affinity Capture-MS Homo sapiens
59 UPF1 5976
Affinity Capture-Western Homo sapiens
60 KPNA3 3839
Affinity Capture-MS Homo sapiens
61 RBM8A 9939
Affinity Capture-MS Homo sapiens
62 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
63 SYNCRIP 10492
Co-fractionation Homo sapiens
64 HNRNPF 3185
Reconstituted Complex Homo sapiens
65 MYCN  
Affinity Capture-MS Homo sapiens
66 SKIV2L2 23517
Affinity Capture-MS Homo sapiens
67 BMI1  
Affinity Capture-MS Homo sapiens
68 FBXW8 26259
Affinity Capture-MS Homo sapiens
69 RC3H1 149041
Affinity Capture-MS Homo sapiens
70 LENG1  
Affinity Capture-MS Homo sapiens
71 SSRP1 6749
Affinity Capture-MS Homo sapiens
72 SF3A2 8175
Affinity Capture-MS Homo sapiens
73 DDX39B 7919
Reconstituted Complex Homo sapiens
74 TIPARP  
Affinity Capture-MS Homo sapiens
75 HNRNPH1 3187
Reconstituted Complex Homo sapiens
76 SNRPC 6631
Affinity Capture-MS Homo sapiens
77 NELFE 7936
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 KPNA4 3840
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 CCDC8  
Affinity Capture-MS Homo sapiens
80 SRRT 51593
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NCBP2 is involved
PathwayEvidenceSource
Abortive elongation of HIV-1 transcript in the absence of Tat IEA Reactome
Axon guidance IEA Reactome
Developmental Biology IEA Reactome
Disease IEA Reactome
Diseases of signal transduction by growth factor receptors and second messengers IEA Reactome
FGFR2 alternative splicing TAS Reactome
FGFR2 mutant receptor activation IEA Reactome
Formation of HIV elongation complex in the absence of HIV Tat IEA Reactome
Formation of HIV-1 elongation complex containing HIV-1 Tat IEA Reactome
Formation of RNA Pol II elongation complex TAS Reactome
Formation of the Early Elongation Complex TAS Reactome
Formation of the HIV-1 Early Elongation Complex IEA Reactome
Gene expression (Transcription) TAS Reactome
HIV Infection IEA Reactome
HIV Life Cycle IEA Reactome
HIV Transcription Elongation IEA Reactome
Infectious disease IEA Reactome
Late Phase of HIV Life Cycle IEA Reactome
Metabolism of non-coding RNA TAS Reactome
Metabolism of RNA TAS Reactome
mRNA 3'-end processing TAS Reactome
mRNA Capping TAS Reactome
mRNA Splicing TAS Reactome
mRNA Splicing - Major Pathway TAS Reactome
mRNA Splicing - Minor Pathway TAS Reactome
Nervous system development IEA Reactome
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) TAS Reactome
Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) TAS Reactome
Nonsense-Mediated Decay (NMD) TAS Reactome
Processing of Capped Intron-Containing Pre-mRNA TAS Reactome
Processing of Capped Intronless Pre-mRNA TAS Reactome
Processing of Intronless Pre-mRNAs TAS Reactome
Regulation of expression of SLITs and ROBOs IEA Reactome
RNA Polymerase II Pre-transcription Events TAS Reactome
RNA polymerase II transcribes snRNA genes TAS Reactome
RNA Polymerase II Transcription TAS Reactome
RNA Polymerase II Transcription Elongation TAS Reactome
RNA Polymerase II Transcription Termination TAS Reactome
Signal Transduction TAS Reactome
Signaling by FGFR TAS Reactome
Signaling by FGFR in disease IEA Reactome
Signaling by FGFR2 TAS Reactome
Signaling by FGFR2 IIIa TM IEA Reactome
Signaling by FGFR2 in disease IEA Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by ROBO receptors IEA Reactome
SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs TAS Reactome
SLBP independent Processing of Histone Pre-mRNAs TAS Reactome
snRNP Assembly TAS Reactome
Tat-mediated elongation of the HIV-1 transcript IEA Reactome
Transcription of the HIV genome IEA Reactome
Transport of Mature mRNA derived from an Intron-Containing Transcript TAS Reactome
Transport of Mature mRNA Derived from an Intronless Transcript TAS Reactome
Transport of Mature mRNAs Derived from Intronless Transcripts TAS Reactome
Transport of Mature Transcript to Cytoplasm TAS Reactome
Transport of the SLBP Dependant Mature mRNA TAS Reactome
Transport of the SLBP independent Mature mRNA TAS Reactome
Viral Infection Pathways IEA Reactome





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