Gene description for Sod1
Gene name superoxide dismutase 1, soluble
Gene symbol Sod1
Other names/aliases B430204E11Rik
Cu/Zn-SOD
CuZnSOD
Ipo-1
Ipo1
SODC
Sod-1
Species Mus musculus
 Database cross references - Sod1
ExoCarta ExoCarta_20655
Vesiclepedia VP_20655
Entrez Gene 20655
UniProt P08228  
 Sod1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Sod1
Molecular Function
    superoxide dismutase activity GO:0004784 IBA
    superoxide dismutase activity GO:0004784 IDA
    superoxide dismutase activity GO:0004784 IMP
    superoxide dismutase activity GO:0004784 ISO
    superoxide dismutase activity GO:0004784 TAS
    copper ion binding GO:0005507 IBA
    copper ion binding GO:0005507 ISO
    copper ion binding GO:0005507 ISS
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 ISO
    zinc ion binding GO:0008270 ISS
    enzyme binding GO:0019899 ISO
    protein phosphatase 2B binding GO:0030346 ISO
    protein phosphatase 2B binding GO:0030346 ISS
    small GTPase binding GO:0031267 IEA
    small GTPase binding GO:0031267 ISO
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 IEA
    protein-folding chaperone binding GO:0051087 ISO
    protein-folding chaperone binding GO:0051087 ISS
Biological Process
    response to reactive oxygen species GO:0000302 IMP
    response to superoxide GO:0000303 IMP
    response to superoxide GO:0000303 ISO
    ovarian follicle development GO:0001541 IMP
    positive regulation of cytokine production GO:0001819 ISO
    positive regulation of cytokine production GO:0001819 ISS
    retina homeostasis GO:0001895 IMP
    myeloid cell homeostasis GO:0002262 IGI
    myeloid cell homeostasis GO:0002262 IMP
    glutathione metabolic process GO:0006749 IMP
    superoxide metabolic process GO:0006801 IMP
    superoxide metabolic process GO:0006801 ISO
    intracellular iron ion homeostasis GO:0006879 IMP
    apoptotic process GO:0006915 IMP
    response to oxidative stress GO:0006979 IDA
    response to oxidative stress GO:0006979 IMP
    response to oxidative stress GO:0006979 ISO
    spermatogenesis GO:0007283 IMP
    embryo implantation GO:0007566 IMP
    sensory perception of sound GO:0007605 IMP
    locomotory behavior GO:0007626 IMP
    anterograde axonal transport GO:0008089 IMP
    retrograde axonal transport GO:0008090 IMP
    regulation of blood pressure GO:0008217 IMP
    determination of adult lifespan GO:0008340 IMP
    response to heat GO:0009408 IMP
    response to xenobiotic stimulus GO:0009410 IMP
    gene expression GO:0010467 IMP
    transmission of nerve impulse GO:0019226 IMP
    neuronal action potential GO:0019228 IMP
    removal of superoxide radicals GO:0019430 IBA
    removal of superoxide radicals GO:0019430 IGI
    removal of superoxide radicals GO:0019430 IMP
    removal of superoxide radicals GO:0019430 ISO
    removal of superoxide radicals GO:0019430 ISS
    removal of superoxide radicals GO:0019430 TAS
    response to nutrient levels GO:0031667 ISO
    peripheral nervous system myelin maintenance GO:0032287 IMP
    positive regulation of superoxide anion generation GO:0032930 IEA
    positive regulation of superoxide anion generation GO:0032930 ISO
    ectopic germ cell programmed cell death GO:0035234 IMP
    regulation of multicellular organism growth GO:0040014 IMP
    response to hydrogen peroxide GO:0042542 IMP
    superoxide anion generation GO:0042554 IDA
    negative regulation of apoptotic process GO:0043066 IDA
    negative regulation of apoptotic process GO:0043066 IMP
    negative regulation of apoptotic process GO:0043066 ISO
    positive regulation of catalytic activity GO:0043085 ISO
    regulation of GTPase activity GO:0043087 ISO
    positive regulation of MAPK cascade GO:0043410 IDA
    negative regulation of neuron apoptotic process GO:0043524 IMP
    response to ethanol GO:0045471 IMP
    negative regulation of cholesterol biosynthetic process GO:0045541 ISO
    regulation of protein kinase activity GO:0045859 ISO
    response to copper ion GO:0046688 ISO
    muscle cell cellular homeostasis GO:0046716 IMP
    response to axon injury GO:0048678 IMP
    hydrogen peroxide biosynthetic process GO:0050665 IMP
    hydrogen peroxide biosynthetic process GO:0050665 ISO
    negative regulation of inflammatory response GO:0050728 IGI
    positive regulation of phagocytosis GO:0050766 IGI
    negative regulation of developmental process GO:0051093 IMP
    regulation of mitochondrial membrane potential GO:0051881 ISO
    regulation of mitochondrial membrane potential GO:0051881 ISS
    heart contraction GO:0060047 IMP
    heart contraction GO:0060047 ISO
    neurofilament cytoskeleton organization GO:0060052 IGI
    neurofilament cytoskeleton organization GO:0060052 IMP
    relaxation of vascular associated smooth muscle GO:0060087 IMP
    auditory receptor cell stereocilium organization GO:0060088 IMP
    reactive oxygen species metabolic process GO:0072593 ISO
    reactive oxygen species metabolic process GO:0072593 ISS
    action potential initiation GO:0099610 IMP
    positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902177 IEA
    positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902177 ISO
    negative regulation of reproductive process GO:2000242 IMP
Subcellular Localization
    extracellular region GO:0005576 ISO
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISO
    extracellular space GO:0005615 ISS
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytoplasm GO:0005737 TAS
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISS
    mitochondrial intermembrane space GO:0005758 TAS
    lysosome GO:0005764 ISO
    peroxisome GO:0005777 IBA
    peroxisome GO:0005777 IEA
    peroxisome GO:0005777 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    secretory granule GO:0030141 ISO
    dense core granule GO:0031045 ISO
    cytoplasmic vesicle GO:0031410 ISO
    cytoplasmic vesicle GO:0031410 ISS
    dendrite cytoplasm GO:0032839 ISO
    dendrite cytoplasm GO:0032839 ISS
    protein-containing complex GO:0032991 ISO
    protein-containing complex GO:0032991 ISS
    neuronal cell body GO:0043025 ISO
    neuronal cell body GO:0043025 ISS
    myelin sheath GO:0043209 HDA
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified Sod1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Sod1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 P3H1 64175
Affinity Capture-MS Homo sapiens
2 PDCD6 10016
Affinity Capture-MS Homo sapiens
3 METTL14  
Affinity Capture-MS Homo sapiens
4 NUDT21 11051
Affinity Capture-MS Homo sapiens
5 RBBP6 5930
Affinity Capture-MS Homo sapiens
6 NCBP1 4686
Affinity Capture-MS Homo sapiens
7 SOD1 6647
Affinity Capture-MS Homo sapiens
8 ZNF346  
Affinity Capture-MS Homo sapiens
9 GAR1 54433
Affinity Capture-MS Homo sapiens
10 NCBP2 22916
Affinity Capture-MS Homo sapiens
11 SLC27A4 10999
Affinity Capture-MS Homo sapiens
12 DNAJB2 3300
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 Kctd13  
Affinity Capture-MS Mus musculus
14 Tfe3  
Affinity Capture-MS Mus musculus
15 C4BPA 722
Affinity Capture-MS Homo sapiens
16 WTAP 9589
Affinity Capture-MS Homo sapiens
17 Tmem173  
Proximity Label-MS Mus musculus
18 SRRT 51593
Affinity Capture-MS Homo sapiens
19 RRAS2 22800
Affinity Capture-MS Homo sapiens
20 WWP2 11060
Affinity Capture-MS Homo sapiens
21 MAPT  
Reconstituted Complex Homo sapiens
22 Als2  
Phenotypic Enhancement Mus musculus
23 FYN 2534
Affinity Capture-MS Homo sapiens
24 SKIV2L2 23517
Affinity Capture-MS Homo sapiens
25 ZC3H18  
Affinity Capture-MS Homo sapiens
26 CCS 9973
Affinity Capture-MS Homo sapiens
27 Invs  
Affinity Capture-MS Mus musculus
28 YES1 7525
Affinity Capture-MS Homo sapiens
29 PRSS1 5644
Affinity Capture-MS Homo sapiens
30 Sqstm1  
Affinity Capture-Western Mus musculus
Co-localization Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
31 Sod1 20655
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
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