Gene description for C4BPA
Gene name complement component 4 binding protein, alpha
Gene symbol C4BPA
Other names/aliases C4BP
PRP
Species Homo sapiens
 Database cross references - C4BPA
ExoCarta ExoCarta_722
Vesiclepedia VP_722
Entrez Gene 722
HGNC 1325
MIM 120830
UniProt P04003  
 C4BPA identified in exosomes derived from the following tissue/cell type
Plasma 19028452    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for C4BPA
Molecular Function
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
Biological Process
    T cell mediated immunity GO:0002456 IBA
    complement activation, classical pathway GO:0006958 IEA
    response to symbiotic bacterium GO:0009609 IDA
    innate immune response GO:0045087 IEA
    positive regulation of protein catabolic process GO:0045732 IDA
    negative regulation of complement activation, classical pathway GO:0045959 IBA
    negative regulation of complement activation, classical pathway GO:0045959 IDA
    regulation of opsonization GO:1903027 IDA
Subcellular Localization
    extracellular region GO:0005576 NAS
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    blood microparticle GO:0072562 HDA
 Experiment description of studies that identified C4BPA in exosomes
1
Experiment ID 44
MISEV standards
✔
IEM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors "Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ"
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
2
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for C4BPA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C5orf15 56951
Affinity Capture-MS Homo sapiens
2 Mtx1  
Affinity Capture-MS Mus musculus
3 Tmed2 56334
Affinity Capture-MS Mus musculus
4 Edc4  
Affinity Capture-MS Mus musculus
5 Tubg1 103733
Affinity Capture-MS Mus musculus
6 AGPAT1 10554
Affinity Capture-MS Homo sapiens
7 Sod1 20655
Affinity Capture-MS Mus musculus
8 SEC16A 9919
Affinity Capture-MS Homo sapiens
9 KIF14 9928
Affinity Capture-MS Homo sapiens
10 SPAG6  
Affinity Capture-MS Homo sapiens
11 PHF11 51131
Affinity Capture-MS Homo sapiens
12 SMARCD1 6602
Two-hybrid Homo sapiens
13 ECT2 1894
Affinity Capture-MS Homo sapiens
14 GTF2I 2969
Two-hybrid Homo sapiens
15 GIT2 9815
Two-hybrid Homo sapiens
16 GNG8  
Affinity Capture-MS Homo sapiens
17 Ccdc12  
Affinity Capture-MS Mus musculus
18 KIF20A 10112
Affinity Capture-MS Homo sapiens
19 FXYD6  
Two-hybrid Homo sapiens
20 TUB  
Affinity Capture-MS Homo sapiens
21 TXN 7295
Reconstituted Complex Homo sapiens
22 APCS 325
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
23 Max  
Affinity Capture-MS Mus musculus
24 ARMC6 93436
Affinity Capture-MS Homo sapiens
25 Sidt2  
Affinity Capture-MS Mus musculus
26 KLK10 5655
Affinity Capture-MS Homo sapiens
27 KIF23 9493
Affinity Capture-MS Homo sapiens
28 Rhoa 11848
Affinity Capture-MS Mus musculus
29 TSNAX 7257
Affinity Capture-MS Homo sapiens
30 MYH11 4629
Affinity Capture-MS Homo sapiens
31 CCR1  
Affinity Capture-MS Homo sapiens
32 Cenpe  
Affinity Capture-MS Mus musculus
33 LONP2 83752
Affinity Capture-MS Homo sapiens
34 LBP 3929
Two-hybrid Homo sapiens
35 UBE2U  
Affinity Capture-MS Homo sapiens
36 Ccdc9  
Affinity Capture-MS Mus musculus
37 Lgals3bp 19039
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which C4BPA is involved
PathwayEvidenceSource
Complement cascade TAS Reactome
Immune System TAS Reactome
Innate Immune System TAS Reactome
Regulation of Complement cascade TAS Reactome





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