Gene description for MYH11
Gene name myosin, heavy chain 11, smooth muscle
Gene symbol MYH11
Other names/aliases AAT4
FAA4
SMHC
SMMHC
Species Homo sapiens
 Database cross references - MYH11
ExoCarta ExoCarta_4629
Entrez Gene 4629
HGNC 7569
MIM 160745
UniProt P35749  
 MYH11 identified in exosomes derived from the following tissue/cell type
Bladder cancer cells 20224111    
Colorectal cancer cells 19837982    
Neuroblastoma cells 25944692    
Prostate cancer cells 25844599    
 Gene ontology annotations for MYH11
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    calmodulin binding GO:0005516 IEA
    actin filament binding GO:0051015 IEA
    motor activity GO:0003774 IEA
    structural constituent of muscle GO:0008307 IMP
Biological Process
    muscle contraction GO:0006936 TAS
    cardiac muscle fiber development GO:0048739 IMP
    activation of mitophagy in response to mitochondrial depolarization GO:0098779 IMP
    smooth muscle contraction GO:0006939 ISS
    small GTPase mediated signal transduction GO:0007264 TAS
    axon guidance GO:0007411 TAS
    ephrin receptor signaling pathway GO:0048013 TAS
    skeletal muscle myosin thick filament assembly GO:0030241 ISS
    elastic fiber assembly GO:0048251 IMP
    metabolic process GO:0008152 IEA
Subcellular Localization
    smooth muscle contractile fiber GO:0030485 IEA
    muscle myosin complex GO:0005859 IEA
    cytosol GO:0005829 TAS
    stress fiber GO:0001725 IEA
    extracellular exosome GO:0070062 IDA
    myosin filament GO:0032982 IEA
    melanosome GO:0042470 IEA
 Experiment description of studies that identified MYH11 in exosomes
1
Experiment ID 76
ISEV standards
EM
EV Biophysical techniques
TSG101|GAPDH|HSP90
EV Cytosolic markers
CD81|CD9|CD63|LAMP1|MHCI
EV Membrane markers
HSP90B1
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 21
ISEV standards
EM|IEM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 275
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|RAB5A
EV Cytosolic markers
CD9|CD82|CD63|CD81
EV Membrane markers
AIF
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for MYH11
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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