Gene description for MPP1
Gene name membrane protein, palmitoylated 1, 55kDa
Gene symbol MPP1
Other names/aliases AAG12
DXS552E
EMP55
MRG1
PEMP
Species Homo sapiens
 Database cross references - MPP1
ExoCarta ExoCarta_4354
Vesiclepedia VP_4354
Entrez Gene 4354
HGNC 7219
MIM 305360
UniProt Q00013  
 MPP1 identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
 Gene ontology annotations for MPP1
Molecular Function
    guanylate kinase activity GO:0004385 TAS
    signaling receptor binding GO:0005102 IBA
    protein binding GO:0005515 IPI
Biological Process
    signal transduction GO:0007165 TAS
    GMP metabolic process GO:0046037 IEA
    GDP metabolic process GO:0046710 IEA
    regulation of neutrophil chemotaxis GO:0090022 ISS
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IBA
    membrane GO:0016020 HDA
    cortical cytoskeleton GO:0030863 IEA
    stereocilium GO:0032420 IEA
    centriolar satellite GO:0034451 IDA
 Experiment description of studies that identified MPP1 in exosomes
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MPP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NCBP1 4686
Affinity Capture-MS Homo sapiens
2 HDAC2 3066
Affinity Capture-MS Homo sapiens
3 SGSM2  
Two-hybrid Homo sapiens
4 MARCKS 4082
Proximity Label-MS Homo sapiens
5 GADD45A  
Two-hybrid Homo sapiens
6 AGFG1 3267
Affinity Capture-MS Homo sapiens
7 ID1  
Affinity Capture-MS Homo sapiens
8 LAMP2 3920
Proximity Label-MS Homo sapiens
9 TRIM55  
Two-hybrid Homo sapiens
10 ZNF584  
Affinity Capture-MS Homo sapiens
11 CEACAM16  
Affinity Capture-MS Homo sapiens
12 CAPN12  
Affinity Capture-MS Homo sapiens
13 RPRM  
Two-hybrid Homo sapiens
14 MAGOHB 55110
Affinity Capture-MS Homo sapiens
15 DGAT2L6  
Two-hybrid Homo sapiens
16 PRDX2 7001
Affinity Capture-MS Homo sapiens
17 MRPS26 64949
Affinity Capture-MS Homo sapiens
18 NR1H2  
Two-hybrid Homo sapiens
19 SARNP 84324
Affinity Capture-MS Homo sapiens
20 TK1 7083
Two-hybrid Homo sapiens
21 ACER2  
Two-hybrid Homo sapiens
22 KCNA4  
Affinity Capture-MS Homo sapiens
23 GSK3B 2932
Two-hybrid Homo sapiens
24 AMOTL2 51421
Two-hybrid Homo sapiens
25 SRP9 6726
Affinity Capture-MS Homo sapiens
26 PRDX5 25824
Affinity Capture-MS Homo sapiens
27 UXT 8409
Two-hybrid Homo sapiens
28 USP21  
Two-hybrid Homo sapiens
29 OPTN 10133
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
30 LYN 4067
Proximity Label-MS Homo sapiens
31 MCAM 4162
Proximity Label-MS Homo sapiens
32 PRPS2 5634
Affinity Capture-MS Homo sapiens
33 HTT 3064
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 TAF9B  
Affinity Capture-MS Homo sapiens
35 ATRAID 51374
Two-hybrid Homo sapiens
36 DIRAS3  
Proximity Label-MS Homo sapiens
37 OGT 8473
Reconstituted Complex Homo sapiens
38 MEOX2  
Two-hybrid Homo sapiens
39 MYH11 4629
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 ACER1  
Two-hybrid Homo sapiens
41 SYNGR3  
Two-hybrid Homo sapiens
42 TRIM63  
Two-hybrid Homo sapiens
43 TNFRSF10A 8797
Affinity Capture-MS Homo sapiens
44 BDH2 56898
Affinity Capture-MS Homo sapiens
45 RPS6KA1 6195
Two-hybrid Homo sapiens
46 XXYLT1 152002
Affinity Capture-MS Homo sapiens
47 HNRNPD 3184
Two-hybrid Homo sapiens
48 STK3 6788
Two-hybrid Homo sapiens
49 ZAK 51776
Two-hybrid Homo sapiens
50 TAF1B  
Affinity Capture-MS Homo sapiens
51 SLC35E1 79939
Two-hybrid Homo sapiens
52 NAF1  
Affinity Capture-MS Homo sapiens
53 FN1 2335
Affinity Capture-MS Homo sapiens
54 CLSPN  
Affinity Capture-MS Homo sapiens
55 AP2M1 1173
Two-hybrid Homo sapiens
56 DKC1 1736
Affinity Capture-MS Homo sapiens
57 ZNF639  
Two-hybrid Homo sapiens
58 FAM13A 10144
Affinity Capture-MS Homo sapiens
59 RPA3 6119
Proximity Label-MS Homo sapiens
60 DNPEP 23549
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
61 COL10A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 KRAS 3845
Proximity Label-MS Homo sapiens
63 STUB1 10273
Two-hybrid Homo sapiens
64 TMEM239  
Two-hybrid Homo sapiens
65 ZNF287  
Affinity Capture-MS Homo sapiens
66 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
67 SMN1 6606
Two-hybrid Homo sapiens
68 TRPS1  
Affinity Capture-MS Homo sapiens
69 FAM102B  
Affinity Capture-MS Homo sapiens
70 TP53 7157
Negative Genetic Homo sapiens
71 BHLHB9 80823
Two-hybrid Homo sapiens
72 PRIMPOL  
Two-hybrid Homo sapiens
73 STK38 11329
Co-fractionation Homo sapiens
74 INCA1  
Two-hybrid Homo sapiens
75 SKP1 6500
Affinity Capture-MS Homo sapiens
76 SQSTM1 8878
Proximity Label-MS Homo sapiens
77 G3BP1 10146
Affinity Capture-MS Homo sapiens
78 MRPS15  
Affinity Capture-MS Homo sapiens
79 AGPAT3 56894
Two-hybrid Homo sapiens
80 PAQR8  
Two-hybrid Homo sapiens
81 PRDX1 5052
Affinity Capture-MS Homo sapiens
82 METTL3  
Affinity Capture-MS Homo sapiens
83 MYH4  
Affinity Capture-MS Homo sapiens
84 ATP5C1 509
Two-hybrid Homo sapiens
85 APH1A 51107
Two-hybrid Homo sapiens
86 KLHL12  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
87 RPS6KA6 27330
Two-hybrid Homo sapiens
View the network image/svg+xml



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