Gene description for WTAP
Gene name Wilms tumor 1 associated protein
Gene symbol WTAP
Other names/aliases Mum2
Species Homo sapiens
 Database cross references - WTAP
ExoCarta ExoCarta_9589
Entrez Gene 9589
HGNC 16846
MIM 605442
UniProt Q15007  
 WTAP identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for WTAP
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    RNA splicing GO:0008380 IEA
    mRNA methylation GO:0080009 IMP
    mRNA processing GO:0006397 IEA
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 IMP
    cell cycle GO:0007049 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nucleus GO:0005634 IDA
    nuclear membrane GO:0031965 IDA
    nuclear speck GO:0016607 IDA
    MIS complex GO:0036396 IDA
 Experiment description of studies that identified WTAP in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for WTAP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 WT1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
2 MAGEA11  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which WTAP is involved
No pathways found





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