Gene description for VPS52
Gene name vacuolar protein sorting 52 homolog (S. cerevisiae)
Gene symbol VPS52
Other names/aliases ARE1
SAC2
SACM2L
dJ1033B10.5
Species Homo sapiens
 Database cross references - VPS52
ExoCarta ExoCarta_6293
Vesiclepedia VP_6293
Entrez Gene 6293
HGNC 10518
MIM 603443
UniProt Q8N1B4  
 VPS52 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for VPS52
Molecular Function
    protein binding GO:0005515 IPI
    syntaxin binding GO:0019905 IBA
    syntaxin binding GO:0019905 IPI
Biological Process
    protein targeting GO:0006605 IEA
    Golgi to vacuole transport GO:0006896 IBA
    lysosomal transport GO:0007041 IBA
    lysosomal transport GO:0007041 IDA
    ectodermal cell differentiation GO:0010668 IEA
    protein transport GO:0015031 IEA
    endocytic recycling GO:0032456 IBA
    endocytic recycling GO:0032456 IMP
    endocytic recycling GO:0032456 NAS
    retrograde transport, endosome to Golgi GO:0042147 IBA
    retrograde transport, endosome to Golgi GO:0042147 NAS
    embryonic ectodermal digestive tract development GO:0048611 IEA
    vesicle-mediated cholesterol transport GO:0090119 IEA
Subcellular Localization
    GARP complex GO:0000938 IBA
    GARP complex GO:0000938 IDA
    GARP complex GO:0000938 NAS
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IEA
    endosome membrane GO:0010008 IEA
    membrane GO:0016020 IDA
    trans-Golgi network membrane GO:0032588 TAS
    perinuclear region of cytoplasm GO:0048471 IDA
    recycling endosome GO:0055037 IDA
    recycling endosome GO:0055037 NAS
    presynapse GO:0098793 IEA
    postsynapse GO:0098794 IEA
    EARP complex GO:1990745 IDA
    EARP complex GO:1990745 IPI
 Experiment description of studies that identified VPS52 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
EM
Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for VPS52
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRKAA1 5562
Two-hybrid Homo sapiens
2 EPM2AIP1 9852
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 RAB1A 5861
Synthetic Lethality Homo sapiens
4 FCER1A  
Affinity Capture-MS Homo sapiens
5 ATG9A 79065
Proximity Label-MS Homo sapiens
6 RABEP2 79874
Two-hybrid Homo sapiens
7 KIFC3 3801
Two-hybrid Homo sapiens
8 C9orf78 51759
Affinity Capture-MS Homo sapiens
9 CCDC132 55610
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 KLC3  
Two-hybrid Homo sapiens
11 TRIM66  
Affinity Capture-MS Homo sapiens
12 KLRK1  
Affinity Capture-MS Homo sapiens
13 BYSL 705
Two-hybrid Homo sapiens
14 VPS54  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
15 CCDC102B  
Two-hybrid Homo sapiens
16 STX11 8676
Two-hybrid Homo sapiens
17 GFM2 84340
Two-hybrid Homo sapiens
18 CWF19L2  
Two-hybrid Homo sapiens
19 WTAP 9589
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
20 KIF5B 3799
Two-hybrid Homo sapiens
21 PPP1R18 170954
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 CLEC2B  
Affinity Capture-MS Homo sapiens
23 CCDC53 51019
Two-hybrid Homo sapiens
24 DDX58 23586
Affinity Capture-RNA Homo sapiens
25 EXD2  
Proximity Label-MS Homo sapiens
26 STX6 10228
Affinity Capture-Western Homo sapiens
27 CABP5  
Two-hybrid Homo sapiens
28 DDX6 1656
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
29 VPS53 55275
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
30 TCP11L1 55346
Two-hybrid Homo sapiens
31 USP2  
Two-hybrid Homo sapiens
32 PKN3 29941
Two-hybrid Homo sapiens
33 MYCN  
Affinity Capture-MS Homo sapiens
34 ARNT2  
Two-hybrid Homo sapiens
35 FAF2 23197
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 LMO4  
Two-hybrid Homo sapiens
37 GPR17 2840
Affinity Capture-MS Homo sapiens
38 CDC20B  
Two-hybrid Homo sapiens
39 EXOC3 11336
Affinity Capture-MS Homo sapiens
40 ENOPH1 58478
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 C1orf216  
Two-hybrid Homo sapiens
42 RAB1B 81876
Synthetic Lethality Homo sapiens
43 NINL  
Proximity Label-MS Homo sapiens
44 C15orf59  
Affinity Capture-MS Homo sapiens
45 HEATR1 55127
Two-hybrid Homo sapiens
46 BSG 682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 KIAA1217 56243
Two-hybrid Homo sapiens
48 VEZF1  
Two-hybrid Homo sapiens
49 RNF41  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
50 MAPK3 5595
Two-hybrid Homo sapiens
51 IKBIP 121457
Affinity Capture-MS Homo sapiens
52 FAM161B  
Two-hybrid Homo sapiens
53 CTDP1 9150
Affinity Capture-MS Homo sapiens
54 VPS51 738
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
55 FAM110A  
Two-hybrid Homo sapiens
56 RABEP1 9135
Affinity Capture-MS Homo sapiens
57 CCDC33 80125
Two-hybrid Homo sapiens
58 SCNM1  
Two-hybrid Homo sapiens
59 PSMD14 10213
Affinity Capture-MS Homo sapiens
60 RAB4A 5867
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
61 NOP2 4839
Two-hybrid Homo sapiens
62 CEP19  
Two-hybrid Homo sapiens
63 VRK1 7443
Co-fractionation Homo sapiens
64 OTUD6A  
Two-hybrid Homo sapiens
65 TBC1D22B  
Two-hybrid Homo sapiens
66 GEM  
Two-hybrid Homo sapiens
67 SREBF2 6721
Negative Genetic Homo sapiens
68 TSSK3  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
69 DTNB 1838
Two-hybrid Homo sapiens
70 NTRK1 4914
Affinity Capture-MS Homo sapiens
71 GORASP2 26003
Two-hybrid Homo sapiens
72 GPR182  
Affinity Capture-MS Homo sapiens
73 PEX14 5195
Proximity Label-MS Homo sapiens
74 C4BPB 725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 MRPL11 65003
Two-hybrid Homo sapiens
76 RAB4B 53916
Two-hybrid Homo sapiens
77 TSTD2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
78 PPM1G 5496
Affinity Capture-MS Homo sapiens
79 CMKLR1 1240
Affinity Capture-MS Homo sapiens
80 SLC2A9  
Affinity Capture-MS Homo sapiens
81 C6orf165  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
82 PTAR1 375743
Synthetic Lethality Homo sapiens
83 TXN2 25828
Two-hybrid Homo sapiens
84 CCDC114  
Affinity Capture-MS Homo sapiens
85 GINS2  
Affinity Capture-MS Homo sapiens
86 EFNA4  
Affinity Capture-MS Homo sapiens
87 CACNA1A  
Two-hybrid Homo sapiens
88 CXCR4 7852
Affinity Capture-MS Homo sapiens
89 TSSC1 7260
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 CCDC185  
Two-hybrid Homo sapiens
91 XPO1 7514
Affinity Capture-MS Homo sapiens
92 CD79B 974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 FAM50B  
Two-hybrid Homo sapiens
94 HOXB5  
Two-hybrid Homo sapiens
95 CCDC57 284001
Two-hybrid Homo sapiens
96 PRKAA2 5563
Two-hybrid Homo sapiens
97 TXLNB  
Two-hybrid Homo sapiens
98 ATP6V1D 51382
Two-hybrid Homo sapiens
99 TRAF6 7189
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
100 CCDC13 152206
Two-hybrid Homo sapiens
101 EGFR 1956
Negative Genetic Homo sapiens
102 AQP3  
Affinity Capture-MS Homo sapiens
103 THAP8  
Affinity Capture-MS Homo sapiens
104 SPACA1 81833
Affinity Capture-MS Homo sapiens
105 MRPL1  
Two-hybrid Homo sapiens
106 MFAP1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
107 MYC  
Affinity Capture-MS Homo sapiens
108 RPA3 6119
Proximity Label-MS Homo sapiens
109 MGC50722  
Two-hybrid Homo sapiens
110 GOLGA1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
111 PSMC5 5705
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 ZNF587  
Two-hybrid Homo sapiens
113 TCEANC  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
114 ZC2HC1C  
Two-hybrid Homo sapiens
115 ZNF417  
Two-hybrid Homo sapiens
116 ALS2CR11  
Two-hybrid Homo sapiens
117 TPM3 7170
Two-hybrid Homo sapiens
118 TXLNA 200081
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
119 HAUS1  
Two-hybrid Homo sapiens
120 CCDC96  
Affinity Capture-MS Homo sapiens
121 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
122 FASN 2194
Negative Genetic Homo sapiens
123 TMEM9 252839
Affinity Capture-MS Homo sapiens
124 NR2C2AP  
Two-hybrid Homo sapiens
125 VPS28 51160
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
126 CCHCR1  
Two-hybrid Homo sapiens
127 VPS52 6293
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
128 ZMAT2  
Two-hybrid Homo sapiens
129 CCDC146  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
130 VAMP4 8674
Affinity Capture-Western Homo sapiens
131 CDK18 5129
Two-hybrid Homo sapiens
132 FAM184A 79632
Two-hybrid Homo sapiens
133 THAP11 57215
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
134 SLC18A2  
Affinity Capture-MS Homo sapiens
135 METTL13 51603
Two-hybrid Homo sapiens
136 SLAMF1 6504
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
137 MKI67  
Affinity Capture-MS Homo sapiens
138 TEAD4  
Two-hybrid Homo sapiens
139 JAKMIP2  
Two-hybrid Homo sapiens
140 FAM161A  
Two-hybrid Homo sapiens
141 C1orf109  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
142 KANK2 25959
Two-hybrid Homo sapiens
143 KXD1 79036
Affinity Capture-MS Homo sapiens
144 CNGA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
145 BRD1 23774
Affinity Capture-MS Homo sapiens
146 ZUFSP 221302
Affinity Capture-MS Homo sapiens
147 SH2D4A 63898
Two-hybrid Homo sapiens
148 NFKBIB  
Two-hybrid Homo sapiens
149 DIABLO 56616
Proximity Label-MS Homo sapiens
150 AAMP 14
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
151 C17orf59 54785
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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