Gene description for VPS51
Gene name vacuolar protein sorting 51 homolog (S. cerevisiae)
Gene symbol VPS51
Other names/aliases ANG2
ANG3
C11orf2
C11orf3
FFR
Species Homo sapiens
 Database cross references - VPS51
ExoCarta ExoCarta_738
Vesiclepedia VP_738
Entrez Gene 738
HGNC 1172
MIM 615738
UniProt Q9UID3  
 VPS51 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for VPS51
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    protein targeting GO:0006605 IEA
    autophagy GO:0006914 IMP
    Golgi organization GO:0007030 IBA
    lysosomal transport GO:0007041 IBA
    lysosomal transport GO:0007041 IMP
    protein transport GO:0015031 IEA
    endocytic recycling GO:0032456 IBA
    endocytic recycling GO:0032456 IMP
    endocytic recycling GO:0032456 NAS
    retrograde transport, endosome to Golgi GO:0042147 IBA
    retrograde transport, endosome to Golgi GO:0042147 IDA
    retrograde transport, endosome to Golgi GO:0042147 IMP
    retrograde transport, endosome to Golgi GO:0042147 NAS
    Golgi vesicle transport GO:0048193 IBA
    brain morphogenesis GO:0048854 IDA
    vesicle-mediated cholesterol transport GO:0090119 IEA
Subcellular Localization
    GARP complex GO:0000938 IBA
    GARP complex GO:0000938 IDA
    GARP complex GO:0000938 NAS
    nucleolus GO:0005730 IDA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IEA
    membrane GO:0016020 IBA
    membrane GO:0016020 IDA
    membrane GO:0016020 TAS
    trans-Golgi network membrane GO:0032588 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
    recycling endosome GO:0055037 IDA
    recycling endosome GO:0055037 NAS
    EARP complex GO:1990745 IBA
    EARP complex GO:1990745 IDA
    EARP complex GO:1990745 IPI
 Experiment description of studies that identified VPS51 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
10
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for VPS51
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAB1A 5861
Synthetic Lethality Homo sapiens
2 METTL7A 25840
Proximity Label-MS Homo sapiens
3 LAMP3  
Proximity Label-MS Homo sapiens
4 ATG9A 79065
Proximity Label-MS Homo sapiens
5 GJA1 2697
Proximity Label-MS Homo sapiens
6 CCDC132 55610
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 GIT2 9815
Affinity Capture-MS Homo sapiens
8 NUP62 23636
Affinity Capture-MS Homo sapiens
9 TRIM66  
Affinity Capture-MS Homo sapiens
10 C9orf78 51759
Affinity Capture-MS Homo sapiens
11 GRIPAP1 56850
Affinity Capture-MS Homo sapiens
12 LAMP2 3920
Proximity Label-MS Homo sapiens
13 RABGAP1L 9910
Affinity Capture-MS Homo sapiens
14 VPS54  
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
15 ARNTL  
Affinity Capture-MS Homo sapiens
16 B3GAT1  
Proximity Label-MS Homo sapiens
17 EXOC1 55763
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 HLA-B 3106
Affinity Capture-MS Homo sapiens
19 FAM174A 345757
Affinity Capture-MS Homo sapiens
20 CDK12 51755
Co-fractionation Homo sapiens
21 NUDT3 11165
Two-hybrid Homo sapiens
22 PSMD9 5715
Affinity Capture-MS Homo sapiens
23 OIP5  
Affinity Capture-MS Homo sapiens
24 HAUS6  
Affinity Capture-MS Homo sapiens
25 STX6 10228
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
26 VPS53 55275
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
27 RAB35 11021
Proximity Label-MS Homo sapiens
28 EBAG9 9166
Proximity Label-MS Homo sapiens
29 GSK3B 2932
Two-hybrid Homo sapiens
30 LAMTOR1 55004
Proximity Label-MS Homo sapiens
31 CCDC114  
Affinity Capture-MS Homo sapiens
32 Arrb2  
Affinity Capture-MS Mus musculus
33 BSG 682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 CLOCK  
Affinity Capture-MS Homo sapiens
35 CDKN1A  
Two-hybrid Homo sapiens
36 PARK2  
Affinity Capture-MS Homo sapiens
37 EXOC4 60412
Affinity Capture-MS Homo sapiens
38 RAB4A 5867
Proximity Label-MS Homo sapiens
39 DTNBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 NTRK1 4914
Affinity Capture-MS Homo sapiens
41 GPR182  
Affinity Capture-MS Homo sapiens
42 DHH  
Affinity Capture-MS Homo sapiens
43 RPA4  
Proximity Label-MS Homo sapiens
44 SNAP29 9342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 YEATS4  
Affinity Capture-MS Homo sapiens
46 NCKAP5L  
Affinity Capture-MS Homo sapiens
47 GINS2  
Affinity Capture-MS Homo sapiens
48 EFNA4  
Affinity Capture-MS Homo sapiens
49 DZIP3  
Affinity Capture-MS Homo sapiens
50 TSSC1 7260
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 AIFM1 9131
Affinity Capture-MS Homo sapiens
52 RAB5A 5868
Proximity Label-MS Homo sapiens
53 CD79B 974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 TNFSF8  
Affinity Capture-MS Homo sapiens
55 LRRC25  
Affinity Capture-MS Homo sapiens
56 NPTN 27020
Affinity Capture-MS Homo sapiens
57 MGARP  
Affinity Capture-MS Homo sapiens
58 PHLDB3  
Affinity Capture-MS Homo sapiens
59 EGFR 1956
Negative Genetic Homo sapiens
60 TFPT  
Affinity Capture-MS Homo sapiens
61 AQP3  
Affinity Capture-MS Homo sapiens
62 PKN3 29941
Affinity Capture-MS Homo sapiens
63 KRT27 342574
Affinity Capture-MS Homo sapiens
64 YTHDC1  
Two-hybrid Homo sapiens
65 ARF6 382
Proximity Label-MS Homo sapiens
66 RPA3 6119
Proximity Label-MS Homo sapiens
67 COG8 84342
Co-fractionation Homo sapiens
68 RAB9A 9367
Proximity Label-MS Homo sapiens
69 PHF21A  
Affinity Capture-MS Homo sapiens
70 TUFT1  
Affinity Capture-MS Homo sapiens
71 RHOB 388
Proximity Label-MS Homo sapiens
72 VIPR1  
Affinity Capture-MS Homo sapiens
73 DUSP16  
Affinity Capture-MS Homo sapiens
74 HMG20A  
Affinity Capture-MS Homo sapiens
75 HUWE1 10075
Affinity Capture-MS Homo sapiens
76 DENND6A 201627
Proximity Label-MS Homo sapiens
77 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
78 CCHCR1  
Affinity Capture-MS Homo sapiens
79 VPS52 6293
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
80 MFSD4  
Affinity Capture-MS Homo sapiens
81 OPALIN  
Affinity Capture-MS Homo sapiens
82 EDEM1  
Affinity Capture-MS Homo sapiens
83 C19orf25  
Affinity Capture-MS Homo sapiens
84 CCDC93 54520
Affinity Capture-MS Homo sapiens
85 RCOR1  
Affinity Capture-MS Homo sapiens
86 ETS2  
Affinity Capture-MS Homo sapiens
87 GOLGA5 9950
Affinity Capture-MS Homo sapiens
88 C16orf72 29035
Affinity Capture-MS Homo sapiens
89 RAB11A 8766
Proximity Label-MS Homo sapiens
90 KXD1 79036
Affinity Capture-MS Homo sapiens
91 ZUFSP 221302
Affinity Capture-MS Homo sapiens
92 KRAS 3845
Proximity Label-MS Homo sapiens
93 AR 367
Affinity Capture-MS Homo sapiens
94 CCDC96  
Affinity Capture-MS Homo sapiens
95 PTRF 284119
Affinity Capture-MS Homo sapiens
96 LURAP1  
Affinity Capture-MS Homo sapiens
97 RPRD1A  
Affinity Capture-MS Homo sapiens
98 C17orf59 54785
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 CDC5L 988
Affinity Capture-MS Homo sapiens
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