Gene description for GRIPAP1
Gene name GRIP1 associated protein 1
Gene symbol GRIPAP1
Other names/aliases GRASP-1
Species Homo sapiens
 Database cross references - GRIPAP1
ExoCarta ExoCarta_56850
Vesiclepedia VP_56850
Entrez Gene 56850
HGNC 18706
MIM 300408
UniProt Q4V328  
 GRIPAP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Neuroblastoma cells 25944692    
 Gene ontology annotations for GRIPAP1
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IEA
    protein binding GO:0005515 IPI
    ionotropic glutamate receptor binding GO:0035255 IEA
    identical protein binding GO:0042802 IPI
Biological Process
    neurotransmitter receptor transport, endosome to postsynaptic membrane GO:0098887 IBA
    regulation of neurotransmitter receptor transport, endosome to postsynaptic membrane GO:0099152 IBA
    regulation of recycling endosome localization within postsynapse GO:0099158 IBA
    negative regulation of receptor clustering GO:1903910 IEA
    regulation of modification of synaptic structure GO:1905244 IBA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 IDA
    axon GO:0030424 IEA
    dendrite GO:0030425 IEA
    presynaptic membrane GO:0042734 IEA
    neuronal cell body GO:0043025 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    recycling endosome membrane GO:0055038 IEA
    blood microparticle GO:0072562 HDA
    postsynaptic recycling endosome GO:0098837 IBA
    glutamatergic synapse GO:0098978 IBA
    extrinsic component of postsynaptic early endosome membrane GO:0098998 IBA
 Experiment description of studies that identified GRIPAP1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for GRIPAP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 NUF2  
Affinity Capture-MS Homo sapiens
3 CCT3 7203
Co-fractionation Homo sapiens
4 CCDC132 55610
Affinity Capture-MS Homo sapiens
5 S100A6 6277
Affinity Capture-MS Homo sapiens
6 GIT2 9815
Affinity Capture-MS Homo sapiens
7 ARL15 54622
Two-hybrid Homo sapiens
8 CCDC183  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 ISCA1  
Affinity Capture-MS Homo sapiens
10 LAMP2 3920
Proximity Label-MS Homo sapiens
11 RABGAP1L 9910
Affinity Capture-MS Homo sapiens
12 STX4 6810
Proximity Label-MS Homo sapiens
13 ARNTL  
Affinity Capture-MS Homo sapiens
14 USO1 8615
Affinity Capture-MS Homo sapiens
15 VPS51 738
Affinity Capture-MS Homo sapiens
16 EXOC1 55763
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 CASP3 836
Biochemical Activity Homo sapiens
18 PSMD9 5715
Affinity Capture-MS Homo sapiens
19 MAGEA9  
Affinity Capture-MS Homo sapiens
20 OIP5  
Affinity Capture-MS Homo sapiens
21 HAUS6  
Affinity Capture-MS Homo sapiens
22 STX6 10228
Proximity Label-MS Homo sapiens
23 VPS53 55275
Affinity Capture-MS Homo sapiens
24 RAB35 11021
Proximity Label-MS Homo sapiens
25 Myh10 77579
Affinity Capture-MS Mus musculus
26 NPAS1  
Affinity Capture-MS Homo sapiens
27 FEM1A  
Affinity Capture-MS Homo sapiens
28 MYL6 4637
Co-fractionation Homo sapiens
29 RABGEF1 27342
Affinity Capture-MS Homo sapiens
30 KRT8 3856
Proximity Label-MS Homo sapiens
31 CLOCK  
Affinity Capture-MS Homo sapiens
32 PCNP 57092
Cross-Linking-MS (XL-MS) Homo sapiens
33 TCP10L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 CYB5D2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 RABEP1 9135
Affinity Capture-MS Homo sapiens
36 RAB4A 5867
Proximity Label-MS Homo sapiens
Two-hybrid Homo sapiens
37 CALB1 793
Affinity Capture-MS Homo sapiens
38 RPA2 6118
Proximity Label-MS Homo sapiens
39 RAD1  
Co-fractionation Homo sapiens
40 TCP10L2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 BICD2 23299
Proximity Label-MS Homo sapiens
42 PKNOX2  
Affinity Capture-MS Homo sapiens
43 CCNT1  
Reconstituted Complex Homo sapiens
44 YEATS4  
Affinity Capture-MS Homo sapiens
45 NCKAP5L  
Affinity Capture-MS Homo sapiens
46 DISC1 27185
Affinity Capture-MS Homo sapiens
47 TCP10  
Affinity Capture-MS Homo sapiens
48 TSSC1 7260
Affinity Capture-MS Homo sapiens
49 RPS2 6187
Co-fractionation Homo sapiens
50 RAB5A 5868
Proximity Label-MS Homo sapiens
51 RAB11A 8766
Proximity Label-MS Homo sapiens
52 PHLDB3  
Affinity Capture-MS Homo sapiens
53 PRKD1 5587
Affinity Capture-MS Homo sapiens
54 RPRD1A  
Affinity Capture-MS Homo sapiens
55 MAP3K1 4214
Biochemical Activity Homo sapiens
56 TFPT  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 METTL21B  
Affinity Capture-MS Homo sapiens
58 PKN3 29941
Affinity Capture-MS Homo sapiens
59 CEP250 11190
Affinity Capture-MS Homo sapiens
60 RPA3 6119
Proximity Label-MS Homo sapiens
61 RAB9A 9367
Proximity Label-MS Homo sapiens
62 PHF21A  
Affinity Capture-MS Homo sapiens
63 TUFT1  
Affinity Capture-MS Homo sapiens
64 PRKY  
Affinity Capture-MS Homo sapiens
65 HMG20A  
Affinity Capture-MS Homo sapiens
66 GRIP1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
67 MGEA5 10724
Co-fractionation Homo sapiens
68 HERC1 8925
Co-fractionation Homo sapiens
69 DZIP3  
Affinity Capture-MS Homo sapiens
70 RPL13 6137
Co-fractionation Homo sapiens
71 LAMTOR1 55004
Proximity Label-MS Homo sapiens
72 MAX  
Affinity Capture-MS Homo sapiens
73 CCHCR1  
Affinity Capture-MS Homo sapiens
74 SPP1 6696
Two-hybrid Homo sapiens
75 HSPB1 3315
Two-hybrid Homo sapiens
76 THAP11 57215
Affinity Capture-MS Homo sapiens
77 CGNL1  
Affinity Capture-MS Homo sapiens
78 DDX58 23586
Affinity Capture-RNA Homo sapiens
79 CCDC93 54520
Affinity Capture-MS Homo sapiens
80 BRK1 55845
Affinity Capture-MS Homo sapiens
81 KDM1A 23028
Affinity Capture-MS Homo sapiens
82 INF2 64423
Affinity Capture-MS Homo sapiens
83 GOLGA5 9950
Affinity Capture-MS Homo sapiens
84 RAB7A 7879
Proximity Label-MS Homo sapiens
85 CLTA 1211
Proximity Label-MS Homo sapiens
86 NDEL1 81565
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 KRT23  
Affinity Capture-MS Homo sapiens
88 HSPA4L 22824
Co-fractionation Homo sapiens
89 CCDC96  
Affinity Capture-MS Homo sapiens
90 PTRF 284119
Affinity Capture-MS Homo sapiens
91 CCT8L2  
Affinity Capture-MS Homo sapiens
92 RCOR1  
Affinity Capture-MS Homo sapiens
93 ZNF365  
Affinity Capture-MS Homo sapiens
94 PARN  
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which GRIPAP1 is involved
No pathways found





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