Gene description for GRIPAP1
Gene name GRIP1 associated protein 1
Gene symbol GRIPAP1
Other names/aliases GRASP-1
Species Homo sapiens
 Database cross references - GRIPAP1
ExoCarta ExoCarta_56850
Entrez Gene 56850
HGNC 18706
MIM 300408
UniProt Q4V328  
 GRIPAP1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Neuroblastoma cells 25944692    
 Gene ontology annotations for GRIPAP1
Molecular Function
    molecular_function GO:0003674 ND
Biological Process
    biological_process GO:0008150 ND
Subcellular Localization
    early endosome GO:0005769 IEA
    blood microparticle GO:0072562 IDA
 Experiment description of studies that identified GRIPAP1 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for GRIPAP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GRIP2 80852
Invivo Homo sapiens
2 CCNT1  
Invivo Homo sapiens
Invitro Homo sapiens
3 ARL15 54622
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which GRIPAP1 is involved
No pathways found





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