Gene description for GRIPAP1
Gene name
GRIP1 associated protein 1
Gene symbol
GRIPAP1
Other names/aliases
GRASP-1
Species
Homo sapiens
Database cross references - GRIPAP1
ExoCarta
ExoCarta_56850
Entrez Gene
56850
HGNC
18706
MIM
300408
UniProt
Q4V328
GRIPAP1 identified in exosomes derived from the following tissue/cell type
Hepatocytes
26054723
Neuroblastoma cells
25944692
Gene ontology annotations for GRIPAP1
Experiment description of studies that identified GRIPAP1 in exosomes
1
Experiment ID
237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocytes
Sample name
MIHA
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RNA Sequencing
2
Experiment ID
224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25944692
Organism
Homo sapiens
Experiment description
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors
Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name
Oncotarget
Publication year
2015
Sample
Neuroblastoma cells
Sample name
SH-SY5Y
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
Protein-protein interactions for GRIPAP1
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
GRIP2
80852
Invivo
Homo sapiens
2
CCNT1
Invivo
Homo sapiens
Invitro
Homo sapiens
3
ARL15
54622
Two-hybrid
Homo sapiens
View the network
image/svg+xml
Pathways in which GRIPAP1 is involved
No pathways found