Gene description for NDEL1
Gene name nudE neurodevelopment protein 1-like 1
Gene symbol NDEL1
Other names/aliases EOPA
MITAP1
NDE1L1
NDE2
NUDEL
Species Homo sapiens
 Database cross references - NDEL1
ExoCarta ExoCarta_81565
Vesiclepedia VP_81565
Entrez Gene 81565
HGNC 17620
MIM 607538
UniProt Q9GZM8  
 NDEL1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NDEL1
Molecular Function
    microtubule binding GO:0008017 IBA
    alpha-tubulin binding GO:0043014 IEA
    beta-tubulin binding GO:0048487 IEA
    protein binding GO:0005515 IPI
    oligopeptidase activity GO:0070012 IEA
Biological Process
    establishment of mitotic spindle orientation GO:0000132 IBA
    inner cell mass cell proliferation GO:0001833 IEA
    neurofilament cytoskeleton organization GO:0060052 IEA
    regulation of intracellular protein transport GO:0033157 IMP
    mitotic centrosome separation GO:0007100 IBA
    centrosome localization GO:0051642 IBA
    neuron projection extension GO:1990138 IEA
    nuclear envelope disassembly GO:0051081 IEA
    positive regulation of axon extension GO:0045773 IEA
    mitotic cell cycle GO:0000278 TAS
    neuron migration GO:0001764 IEA
    positive regulation of axon regeneration GO:0048680 IEA
    chromosome segregation GO:0007059 IMP
    retrograde axon cargo transport GO:0008090 IEA
    regulation of neuron projection development GO:0010975 IBA
    small GTPase mediated signal transduction GO:0007264 TAS
    central nervous system neuron axonogenesis GO:0021955 IEA
    establishment of chromosome localization GO:0051303 IBA
    cerebral cortex radially oriented cell migration GO:0021799 IEA
    positive regulation of GTPase activity GO:0043547 IMP
    vesicle transport along microtubule GO:0047496 IBA
    proteolysis GO:0006508 IEA
    cell migration GO:0016477 IBA
    microtubule nucleation GO:0007020 IBA
Subcellular Localization
    nucleolus GO:0005730 IDA
    cytosol GO:0005829 TAS
    kinesin complex GO:0005871 IBA
    axon hillock GO:0043203 IEA
    condensed chromosome kinetochore GO:0000777 IEA
    neurofilament cytoskeleton GO:0060053 IEA
    spindle GO:0005819 IEA
    cell leading edge GO:0031252 IEA
    centrosome GO:0005813 IBA
    cytoplasm GO:0005737 IDA
    microtubule GO:0005874 IEA
    nuclear envelope GO:0005635 IEA
    kinetochore GO:0000776 IDA
 Experiment description of studies that identified NDEL1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NDEL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAE 7531
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
2 DYNC1H1 1778
Affinity Capture-Western Homo sapiens
3 DISC1 27185
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 MBIP  
Two-hybrid Homo sapiens
5 PAFAH1B1 5048
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
6 CDK5 1020
Biochemical Activity Homo sapiens
Co-localization Homo sapiens
7 MAGEA11  
Two-hybrid Homo sapiens
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