Gene description for SYNE1
Gene name spectrin repeat containing, nuclear envelope 1
Gene symbol SYNE1
Other names/aliases 8B
ARCA1
C6orf98
CPG2
EDMD4
MYNE1
Nesp1
SCAR8
dJ45H2.2
Species Homo sapiens
 Database cross references - SYNE1
ExoCarta ExoCarta_23345
Vesiclepedia VP_23345
Entrez Gene 23345
HGNC 17089
MIM 608441
UniProt Q8NF91  
 SYNE1 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Plasma 19028452    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for SYNE1
Molecular Function
    actin binding GO:0003779 IDA
    protein homodimerization activity GO:0042803 ISS
    actin filament binding GO:0051015 ISS
    protein binding GO:0005515 IPI
    lamin binding GO:0005521 IPI
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    muscle cell differentiation GO:0042692 IDA
    establishment of nucleus localization GO:0040023 IEA
    nucleus organization GO:0006997 NAS
    cytoskeletal anchoring at nuclear membrane GO:0090286 IDA
    nuclear matrix anchoring at nuclear membrane GO:0090292 IDA
    Golgi organization GO:0007030 IDA
Subcellular Localization
    cytoskeleton GO:0005856 IEA
    nucleoplasm GO:0005654 IDA
    integral component of membrane GO:0016021 IDA
    nuclear outer membrane GO:0005640 IEA
    postsynaptic membrane GO:0045211 IDA
    LINC complex GO:0034993 IDA
    Golgi apparatus GO:0005794 IDA
    nuclear envelope GO:0005635 IDA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    nuclear membrane GO:0031965 IDA
    sarcomere GO:0030017 IDA
 Experiment description of studies that identified SYNE1 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 44
MISEV standards
✔
IEM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 196
MISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 197
MISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SYNE1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DISC1 27185
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 ACTC1 70
Invivo Homo sapiens
3 LMNA 4000
Affinity Capture-Western Homo sapiens
4 MUSK  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
5 EMD 2010
Invitro Homo sapiens
Invitro Homo sapiens
6 SYNE1 23345
Invitro Homo sapiens
Two-hybrid Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which SYNE1 is involved
PathwayEvidenceSource
Meiotic synapsis TAS Reactome





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